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Citations for Takara Bio :
6101 - 6150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Lentivirus was made by transfecting LentiX-293T (Takara Bio) cells with Lenti-X Packaging Single Shot (Takara Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... and pRb-TA-Luc (Clontech/Takara) (promoter element directly suppressed by Rb) ...
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bioRxiv - Cancer Biology 2023Quote: ... and pRb-TA-Luc (Clontech/Takara) (promoter element directly suppressed by Rb) ...
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bioRxiv - Synthetic Biology 2023Quote: ... His-tagged proteins were purified with His60 Ni Superflow resin (TaKaRa; 635677) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: As a basis for minimal lentiviral vectors the pLVX-IRES-Neo vector (Clontech) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were concentrated using the LentiX Concentrator (Clontech). Organoids were trypsinized into small cell clusters and incubated with the concentrated virus particles supplemented with 4 μg/mL Polybrene (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus reagent (TaKaRa), and cDNA was synthesized from 0.5 µg of each RNA preparation using the ReverTra Ace qPCR RT kit (TOYOBO ...
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bioRxiv - Neuroscience 2023Quote: ... and SHANK3-3D (T551D/S694D/S781D) were generated with a PrimeSTAR Mutagenesis Basal Kit (Takara, Shiga, Japan). pGEX-14-3-3ζ ...
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bioRxiv - Neuroscience 2023Quote: ... and shLGR5 was cloned into the pSiren-RetroQ-ZsGreen vector (Clontech). For lentivirus preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.16 U/μl Poly-A-polymerase (2180A, Takara), 3.3 U/μl SuperaseIn RNase inhibitor (AM 2694 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10-μL TB Green Premix Ex Taq II (Takara, Beijing, China), and 7-μL sterile deionized water ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloned into pMD19-T vector (Takara Bio, Dalian, China) before transformation into E ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was added to the Ni-IDA resin (Takara, Cat # 635660) equilibrated with Binding Buffer (50 mM NaPi ...
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bioRxiv - Microbiology 2023Quote: ... 5’RACE was performed with SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc. San Jose, CA USA) according to the manufacturer’s directions.
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bioRxiv - Molecular Biology 2023Quote: ... including PCR using PrimeSTAR GXL (Takara), and DpnI treatment (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% Tet system-approved FBS (Takara #631368), 50 µg/mL hygromycin B ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative RT-PCR for pluripotency and trilineage spontaneous differentiation was performed according to the instruction manual of the human ES cell Primer Array (Takara Clontech).
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bioRxiv - Cell Biology 2023Quote: ... using the In-Fusion HD cloning kit (Takara Bio, Shiga, Japan). SART1 mutants were generated using the KOD-Plus-Mutagenesis kit (Toyobo ...
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bioRxiv - Cell Biology 2023Quote: ... followed by template digestion with DpnI restriction enzyme (Takara, #1235A). The amplified product was transformed in Escherichia coli NEB5α cells (NEB#C2987 ...
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bioRxiv - Developmental Biology 2023Quote: ... Then ∼200 ng of DNA was digested with McrBC (Takara, 1234A, Japan) for 18 h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Lentiviral particles were produced by co-transfecting the barcoding plasmid with the packaging and envelope plasmids pMD2G and pCMV-dR8.91 (provided by Andrew Ramos) into Lenti-X 293T cells (Takara, 632180) with Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... Viral supernatant was concentrated using Lenti-X Concentrator (Takara, 631232).
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bioRxiv - Immunology 2023Quote: ... Libraries were prepared at La Jolla Institute for Immunology using SMARTer Stranded RNA-Seq kit (Takara 634962) and were sequenced using an Illumina HiSeq2500 in Rapid Run Mode with paired end 50 reads.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Physiology 2023Quote: ... Up to 30 ng of post-Immunoprecipitation dsDNA as measured by Invitrogen™ Qubit™ highsensitivity spectrofluorometric measurement was made into Illumina-compatible NGS libraries using the ThruPLEX® DNA-Seq kit from Takara Bio Inc ...
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bioRxiv - Neuroscience 2023Quote: ... the solution was replaced with a 0.5% BSA and 0.2% Triton X/PBS solution (PBST) containing a 1:1,000 dilution of Rabbit anti-DsRed (Takara, #632496) or Chicken Polyclonal anti-GFP antibody (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were prepared using SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) and sequenced by NovaSeq (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
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bioRxiv - Neuroscience 2023Quote: Each lysis plate well contained 4uL of lysis buffer (4U Recombinant RNase Inhibitor (Takara Bio 2313B), 0.05% Triton X-100 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cDNA was synthesized from 100 µg of total RNA by Prime ScriptTM RT reagent kit with gDNA Eraser (Takara, Tokyo, Japan). Each cDNA sample was amplified for the gene of interest and β-actin in a 15 µL reaction volume TB GreenTM Premix Ex Taq™ II (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR reactions were implemented in an Applied Biosystems 7500 Real-Time PCR system with the SYBR® Premix Ex TaqTM II (TAKARA). ACTIN was used as the reference gene for qPCR analyses ...
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bioRxiv - Microbiology 2023Quote: ... Amplified fragments were purified using 0.55x beads/sample ratio NucleoMag® NGS magnetic beads (Takara Bio). Amplicons were prepared for Oxford Nanopore sequencing using the ligation sequencing kit SQK-LSK110 (Oxford Nanopore Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... OsRGB1 was amplified with flanking NotI and BamHI sites to clone into the NotI/BglII sites of pBridge MCS2 (Takara) as the “bridge” protein ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was titered using the Lenti-X p24 Rapid Titer Kit (Takara Cat #632200).
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bioRxiv - Neuroscience 2023Quote: rabbit α-dsRed (Takara, Living Colors 632496) at 1:1000
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bioRxiv - Bioengineering 2023Quote: ... RNA was converted to cDNA using SmartSeq-v4 (Takara, cat no. R400752). The library was prepared using Nextera XT DNA library preparation kit (Illumina ...
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bioRxiv - Biophysics 2023Quote: ... The cDNA encoding Keratin 14 R125C-YFP was a kind gift of Thomas Magin (University of Leipzig, Germany) and was cloned into a retroviral vector (pTRE, Takara Clontech). Retrovirus was generated as described in [44] and transduced into MDCK cells ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-dsRed (Clontech, 632496, 1:250), mouse anti-HuC/D (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was prepared using the PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa Bio). The first targeted PCR was performed using a KAPA HiFi HotStart ReadyMix PCR Kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Amplification was performed using the Tks Gflex DNA Polymerase Low DNA (Takara Bio). Thermal cycles were as follows ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was performed using the TB Green Fast qPCR Mix (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Real-time RT-PCR was performed using SYBR green (Takara) in QuantStudio3 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... Uncleaved protein and cleaved His6-Ztag domains were separated from cleaved protein by incubation with TALON resin (Clontech). The supernatant was buffer exchanged to MES A buffer (20 mM MES ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was carried out using TB Green® Premix Ex Taq™ GC (TaKaRa, RR071Q). Gene expression data were normalized to sigA and expressed as fold change using the 2−ΔΔCt method compared to wild-type strain or untreated control.
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bioRxiv - Microbiology 2023Quote: ... All PCRs for genetic modifications were performed with a high-fidelity DNA polymerase (PrimeStarMax, Takara) and chromosomal modifications were confirmed by colony PCR (Dream Taq ...
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bioRxiv - Microbiology 2023Quote: Sequence of the mouse variable heavy and kappa chains were obtained by using SMARTer 5’ RACE technology (Takara Bio, USA) adapted for antibodies to amplify the variable genes from heavy and kappa chains for each hybridoma based on isotype ...
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bioRxiv - Microbiology 2023Quote: ... we further concentrated virus stocks using Lenti-X™ Concentrator (Takara, 631232) to between 3.5*10^5-1.5*10^6 TU/ml.