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Citations for Takara Bio :
6051 - 6100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... First stand cDNA synthesis was performed using SmartScribe Reverse Transcriptase (Takara Bio USA #639538), and RNA was removed ...
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bioRxiv - Cell Biology 2023Quote: ... Doxycycline is from Takara. Calcium chloride is from Acros Organics ...
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bioRxiv - Cell Biology 2023Quote: ... The media was harvested 72 h post-transfection, filtered through a 0.45 μm filter (Millapore, Catalog #SLHVM33RS) and concentrated using LentiX (Takara Biosciences, Catalog #631232). Concentrated lentivirus was added to cells on day 2 of differentiation ...
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bioRxiv - Cell Biology 2023Quote: HaCaT cells were treated with the E-cadherin-blocking antibody (SHE78-7, Takara, Shiga, Japan), PY-60 (Axon Medchem ...
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bioRxiv - Cell Biology 2023Quote: ... about ∼5 μg of bacmid were transfected using 5 μL of TransIT®-Insect transfection reagent (Takara Bio Inc.). 5 days after initial transfection ...
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bioRxiv - Cell Biology 2023Quote: ... concentrated with Lenti-X Concentrator (Clontech), and the pellet was dissolved in ADF medium (Thermo Fischer #12634) ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using PrimeScript™ RT Reagent Kit (Takara, Tokyo, Japan). qRT-PCR was performed on a Mx3000P real-time PCR system (Stratagene ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used for the study: mouse anti-GFP (632381, JL-8, Clontech, 1:1000), rabbit anti-mCherry (GTX128508 ...
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bioRxiv - Cell Biology 2023Quote: ... or pMSCV (Clontech). Drug resistance genes in lentiGuide and pLenti were replaced by blasticidin S ...
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bioRxiv - Cell Biology 2023Quote: ... or In-Fusion HD Cloning Plus (Takara 638909). Site-directed mutagenesis was performed by QuikChange Site-directed Mutagenesis Kit (Agilent 200523) ...
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bioRxiv - Cell Biology 2023Quote: ... doxycycline (0.03 µg/mL, 24 hours; TAKARA) and Biotin (500 µM ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNA fragments were amplified using PrimeSTAR HS DNA polymerase (TAKARA, Japan), and plasmid recombination was performed using NEBuilder HiFi DNA assembly (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... Expression plasmids encoding mCherry-tagged PDZ-RhoGEF and its mutants were constructed by inserting the PCR-amplified cDNA fragments into an mCherry-C1 expression plasmid (Clontech). The cDNA fragments were amplified using PrimeSTAR HS DNA polymerase (TAKARA ...
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bioRxiv - Cell Biology 2023Quote: ... The culture medium for SGE2 consisted of NDiff 227 (Y40002, Takara) supplemented with 1000 U/mL LIF (ESG1107 ...
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bioRxiv - Cell Biology 2023Quote: ... into ECFP-C1 expression plasmid (Clontech).
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bioRxiv - Cell Biology 2023Quote: ... The culture medium for rESCs consisted of NDiff 227 (Y40002, Takara) supplemented with 1000 U/mL LIF (ESG1107 ...
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bioRxiv - Cell Biology 2023Quote: ... 6-well plates of 70% confluent Lenti-X 293T cells (Clontech) were transfected with 1.5 μg transfer vector ...
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bioRxiv - Cell Biology 2023Quote: ... mouse MEFs (RIKEN BioResource Center, Tsukuba, Japan), mouse RAW264.7 macrophages (RIKEN BioResource Center, Tsukuba, Japan) and Lenti-X 293T (Clontech) were cultured at 37°C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using PrimeScript RT Master Mix (TAKARA). Real-time quantitative PCR was performed with CFX384 Touch (Bio Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Doxycycline (Dox, 631311) was purchased from Clontech. Lipids (egg SM and egg PC ...
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bioRxiv - Cell Biology 2023Quote: The lenti-CRISPR v2 constructs containing specific sgRNAs were transfected into Lenti-X 293T cells (Takara; 632180) with psPAX2 and VSV-G plasmids using the FuGENE HD transfection reagent (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... GSL-glycans were isolated by enzymatic digestion using EGCase I (Takara Bio Inc. Japan), whereas fOSs were recovered from an EGCase I-free fraction (46) ...
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bioRxiv - Cell Biology 2023Quote: ... and SYBR Premix Ex Taq II (Takara, Shiga, Japan) with gene-specific primers (S4 Table ...
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bioRxiv - Cell Biology 2023Quote: Sequence confirmed cDNAs encoding Snail and OVOL2 were obtained from the DNASU plasmid repository (Arizona State University) and subcloned into pLVX-TetOne-Puro (Takara) vector ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (Clontech) were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral vectors for expression of LDHA or LDHB were generated by PCR amplification of the respective cDNAs from plasmids obtained from the Eugene McDermott Center for Human Growth and Development Sequencing Core Human ORF Collection and cloning into pLVX-IRES-Puro (Takara 632183) through Gibson assembly (NEB E2621) ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNA was diluted for suitable fold and mixed with qPCR SYBR Green Master Mix (Takara) and primer pairs ...
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bioRxiv - Cell Biology 2023Quote: ... 500 ng total RNA was reversed transcribed into cDNA using the cDNA synthesis kit (Takara). The cDNA was diluted for suitable fold and mixed with qPCR SYBR Green Master Mix (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... and pVSV-G (#PT3343-5, Clontech) vectors were transfected using Lipofectamine 3000 ...
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bioRxiv - Cell Biology 2023Quote: ... digested with EcoRI using the In-Fusion HD Cloning Kit (Takara Bio). Then ...
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bioRxiv - Cell Biology 2023Quote: ... as a template and inserted into pEGFP-C2 vector (Takara Bio) digested with EcoRI using the In-Fusion HD Cloning Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... and RNA-seq libraries were generated using the SMARTer ThruPLEX DNA-Seq Kit (Takara Bio, Cat. # R400674). Each library was indexed using the DNA Unique Dual Index Kit (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: All viruses and lentiviruses were produced by transfecting Hek293T cells using CalPhos Mammalian Transfection Kit (Takara Bio) according to the manual in DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... Lenti-X cells (Clontech) were transfected with pMD2.G (addgene #12259) ...
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bioRxiv - Cell Biology 2023Quote: ... Contaminant DNA in 50 µg of extracted RNA was digested with DNaseI (cat. #2270A, Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Viral supernatants were collected and concentrated 20x using Retro-X concentraror (Takara Bio, PT5063-2), and stored at -80°C ...
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bioRxiv - Cell Biology 2023Quote: ... The synthesized sequences were ligated to an expression construct encoding a truncated form of OsPHR (Δ1-51OsPHR) using restriction enzyme cloning or InFusion cloning (InFusion HD Cloning kit, Takara Bio, Shiga, Japan). The modified PHR was cloned into the binary vector pMpGWB106 (Ishizaki et al. ...
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bioRxiv - Cell Biology 2023Quote: ... we used the pRetroX-Tet-on-advanced and pRetrox-Tight-Puro plasmids from Clontech Inc ...
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bioRxiv - Cell Biology 2023Quote: ... Clonal lines were genotyped by PCR using EmeraldAmp GT PCR Master Mix (Takara, RR310A) and target specific primers flanking the Myrlin transcription start site ...
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bioRxiv - Cell Biology 2023Quote: ... Y2H studies were based on the Yeast protocols handbook (Clontech, Protocol No ...
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bioRxiv - Cell Biology 2023Quote: ... 12.5 µL of Tks Gflex DNA polymerase Low DNA (cat. #R091A, Takara) and 12.2 µL of NFW} was added ...
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bioRxiv - Cell Biology 2023Quote: Procollagen Type 1 C-peptide (PIP) was measured according to the manufacturer’s instructions (Takara, Shiga, Japan). For evaluation of PIP ...
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bioRxiv - Cell Biology 2023Quote: ... or In-Fusion cloning (Takara Bio). Full-length sequences of plasmids used in this study will become available at a public data share server upon publication.
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bioRxiv - Cell Biology 2023Quote: ... 1.6 µg of a transfer plasmid was transfected into LentiX 293T cells (Clontech Laboratories, inc.) with 0.8 µg of psPAX2 (addgene #12260 ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were generated using Gibson assembly and In-Fusion cloning according to manufacturer protocols (Takara, 638920). PB-NCT-GFP plasmids were generated using NCT plasmids donated by Zane Zeier and were flanked by PiggyBac transposon arms ...
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bioRxiv - Developmental Biology 2023Quote: ... The amplicon was then inserted into the pUASz-tdTomato vector using the In-fusion cloning technique (Takara) following digestion with AgeI ...
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bioRxiv - Developmental Biology 2023Quote: ... using In-fusion cloning (Takara), we subcloned into the pHD-tdTomato-DsRed-attP vector the two homology arms (HAs ...
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bioRxiv - Developmental Biology 2023Quote: This line was generated using the In-fusion cloning technique (Takara) in two steps as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... HA hRB1 delta CDK was subcloned at BamHI restriction site on pIRES2-EGFP vector (Clontech). The Mcidas-GFP construct was a gift from S Taraviras ...
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bioRxiv - Developmental Biology 2023Quote: Sample libraries were prepared with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech Kit Cat# 63488). Libraries were sequenced on a NovaSeq 6000 (Illumina ...