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Citations for Takara Bio :
6001 - 6050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The libraries for Illumina sequencing were constructed using the ThruPLEX DNA-Seq Kit (Clontech) and purified using SPRIselect beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2023Quote: For the synthesis of cDNA with random hexamers (PrimeScript RT reagent kit from Takara Bio) 300 ng of total RNA were used ...
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bioRxiv - Bioengineering 2023Quote: ... All cloning was performed using the In-Fusion Cloning kit (Takara Bio) and ligated plasmids were transformed into chemically competent Stbl3 E ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... PrimeStar max DNA polymerase (Takara Bio, #R045A) and barcoded primers (Supplementary Table 1 ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Genomics 2023Quote: ... We extracted high-molecular-weight (HMW) DNA using Nucleobond® HMW DNA extraction kit (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... 18-20 μg of indicated plasmids were transfected in HEK293T cells using the CalPhos Mammalian Transfection Kit (Takara). After 48h ...
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bioRxiv - Developmental Biology 2023Quote: ... and concentrated with Lenti-X Concentrator (Cat. # 631232, Takara). The resulting pellet was resuspended in mTesR+ (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... following ribosomal RNA removal using RiboGone (Takara catalog: 634846). Sequencing was performed on an Illumina HiSeq 2500 with 40 million 75bp paired-end reads ...
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bioRxiv - Immunology 2023Quote: ... M-IgA was expressed by transfecting a pair of alpha heavy and kappa light chain genes into FreeStyle CHO-S cells that were grown in a 24-well plate according to the manufacturer’s protocol (CHOgro High Yield Expression System, Takara Bio, Shiga, Japan). For large-scale antibody production ...
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bioRxiv - Cancer Biology 2023Quote: ... and mouse anti-SPARC/Osteonectin (ON1-1, Takara) for 1 h at RT ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... the Tet-On® 3G Inducible Expression System kit was used (Takara Bio; 631167). RPE-1 wild-type and CKAP2-KO cells were transfected with a pEF1α-TET3G plasmid for expression of a Tet-On 3G transactivator protein ...
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bioRxiv - Cancer Biology 2023Quote: ... with the exception that 1U/μL of RNAse inhibitor (Takara Bio, #2313A) was added to all buffers during and after nuclei isolation (i.e ...
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bioRxiv - Cancer Biology 2023Quote: ... above 20% were used for downstream library generation using the SMARTer Stranded Total RNA – Pico RNA-seq kit v2 (Takara). Libraries were sequenced using 100 nucleotide paired-end sequencing on a NovaSeq 6000 (Illumina).
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bioRxiv - Cancer Biology 2023Quote: ... Clarified lysate was mixed with ∼0.5 ml/L of culture cobalt resin (TaKaRa) for one hour ...
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bioRxiv - Cancer Biology 2023Quote: ... TP63 cDNA constructs were cloned into a lentiviral IRES-Neo vector using the In-Fusion cloning system (Takara). A 3×FLAG tag was added to TP63 at the N- or C-terminus ...
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bioRxiv - Cell Biology 2023Quote: ... using In-Fusion HD (Takara Bio). ACs C-terminally tagged with muGFP44 were generated by removing EGFP from pEGFP-N1 vector by digestion with AgeI and NotI followed by PCR amplification of muGFP (a gift from Mullins lab ...
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bioRxiv - Evolutionary Biology 2023Quote: ... by In-Fusion cloning (Takara Bio). Verified plasmids were then introduced into Agrobacterium strain AGL1 by a freeze-thaw method (85) ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed q-RTPCR using TB Green Premix Ex Taq Ⅱ (Takara, Japan) with specific primers (Supplemental Table 1) ...
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bioRxiv - Cell Biology 2023Quote: Human bone marrow mesenchymal stem cells (BM MSCs) (TaKaRa Bio) were cultured in Mesenchymal Stem Cell Growth Medium 2 (TaKaRa Bio ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T-LentiX (Takara Bio, cat.no. 632180) and HeLa (Unauthenticated HeLa-Kyoto ...
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bioRxiv - Neuroscience 2023Quote: ... ds-cDNA was generated using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... cerevisiae AH109 (Takara Clontech Bio Europe ...
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bioRxiv - Plant Biology 2023Quote: ... thaliana (Takara Clontech Bio Europe, Cat.# 630487). For direct interaction studies co-transformation of bait and pray was employed ...
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bioRxiv - Immunology 2023Quote: ... SYBR TB Green Premix Ex Taq (Takara, #RR820W) and primers supplied by Sigma Aldrich (see table 1 ...
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bioRxiv - Immunology 2023Quote: ... concentrated with Lenti-X Concentrator (Takara), used to transduce K562 cells with an MOI of 20 ...
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bioRxiv - Microbiology 2023Quote: ... Cloning was performed with In-Fusion (Takara Bio, Shiga, Japan) according to manufacturer protocol and transformed in chemically competent E ...
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bioRxiv - Genomics 2023Quote: ... Target regions were also amplified using genomic DNA and Takara EX Taq Hot Start Version (Takara Bio). Sanger sequencing was performed using a conventional Sanger sequencing service (Fasmac ...
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bioRxiv - Genomics 2023Quote: ... and 1 ng of embryonic total RNA from rabbits and cattle were reverse transcribed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio, Japan) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed using TaKaRa Ex Taq Hot Start Version (TaKaRa) with primers designed at 3’UTR of wallaby ZDBF2 according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription and amplification were performed using One Step PrimeScript™ RT-PCR Kit (Takara, Japan) and reaction performed and visualized using Stratagene Mx3000P qPCR System real-time PCR system (Agilent ...
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bioRxiv - Microbiology 2023Quote: An LHCX MLV vector (Clontech) was engineered to express the N-terminal domains (RIGN ...
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bioRxiv - Microbiology 2023Quote: ... HD In-Fusion kit (Takara 638909 In-Fusion® HD Cloning Plus) was used for cloning.
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bioRxiv - Microbiology 2023Quote: ... Mutation D382A in NP was generated with the In-Fusion cloning method (Takara 638909 In- Fusion® HD Cloning Plus). rLCMV/NP(D382A ...
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bioRxiv - Immunology 2023Quote: ... T cells were transduced by centrifugation on Retronectin (Takara)-coated plates.
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bioRxiv - Immunology 2023Quote: ... Viral supernatants were concentrated 10-fold using RetroX concentrator (Takara).
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bioRxiv - Microbiology 2023Quote: ... these four fragments were fused together using the In-Fusion system (Takara Bio, Inc., Japan).
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bioRxiv - Immunology 2023Quote: Lenti-XTM 293T cells (Takara) were cultured at 0.5-1x106 cells/ml in Dulbecco’s modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... pLKO.1-shFEN1 or pLKO.1 control vector was simultaneously transfected into LentiX293T (Cat# 632180 Clontech, Japan) with packaging plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... or 100 ng of genomic DNA was amplified with Advantage GC 2 polymerase (Takara Bio 639114) using 1 M GC Melt and primers spanning the region from upstream of the splice donors to the 3’ end of the repeat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries construction (SMART-Seq v4 Ultralow input, Takara) with barcoded adaptors as well as RNA sequencing on NextSeq500-Illumina (single-end 1×5 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting cDNAs were PCR-amplified using LA Taq DNA polymerase (TaKaRa), a common PCR primer used for all library amplifications ...
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bioRxiv - Developmental Biology 2023Quote: ... First strand cDNA was prepared using the TAKARA PrimeScript RT Master Mix (TAKARA RR036A). Real-time PCR primers (Table S8 ...
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bioRxiv - Cell Biology 2023Quote: ... The PISD-FLAG plasmid was generated by amplifying human PISD from cDNA and cloning it into the BglII and SalI sites of the pmCherry-N1 vector (Clontech). The C-terminal mCherry tag was subsequently replaced with 3xFLAG tag using the AgeI and NotI restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: ... Two-color FISH was conducted as previously described with minor adaptations.20,21 Undifferentiated ChiPSC22 were seeded on DEF-CS COAT-1-coated (Cellartis, Takara BioSciences) coverslips and differentiated ChiPSC22 on poly-L-lysine-coated coverslips ...
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bioRxiv - Cell Biology 2023Quote: ... and libraries were generated by LIANTI40 or PicoPLEX Gold (Takara Bio). Libraries were sequenced by NOVAseq (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: Human DHRS7 was expressed as a GFP fusion using the pEGFP-N2 vector (Clontech) generated previously [5] ...
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bioRxiv - Cancer Biology 2023Quote: ... was cultivated in the feeder-free DEF-CS system (Cellartis/Takara Bio Europe) under standard conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... Constructs were ligated (In-Fusion HD, Takara) into pLenti vector behind an EFS promoter using BamHI and NsiI sites ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 ng/mL doxycycline (Takara Bio) was added to the media and cells were incubated for 48 h at 37°C ...