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Citations for Takara Bio :
5951 - 6000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cDNA reactions were performed according to the manufacturer’s instructions of the PrimeScript™ RT Reagent Kit (Takara) using primers specifically targeting the U6 and scaffold region (forward primer 5’-CCGACTCGGTGCCACTTTTT-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... and InFusion cloning (Takara Bio). For the JUN AUG mutants ...
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bioRxiv - Molecular Biology 2023Quote: HeLa (cTT20.1166, gift from Kara L. McKinley and Iain Cheeseman) and Lenti-X HEK293T cells (Takara Bio, used for lentivirus production) were cultured in DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... was grown in RPMI 1640 medium supplemented with 10% Tet System Approved FBS (TaKaRa), penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by the transfection with Xfect mESC transfection reagent (Takara 631317).
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bioRxiv - Molecular Biology 2023Quote: ... containing VSW-3 RNA polymerase promoter for positive-sense and negative-sense strand ssRNA were amplified by a three-step PCR using PrimeSTAR Max DNA Polymerase (Takara, Japan) (primers are listed in Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’UTR synthetic intron fragment was synthesized (Twist Biosciences) and cloned into the AgeI restriction site in all reporter plasmids via InFusion (Takara). These plasmids are available through Addgene (Addgene ID#s available upon publication).
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were sized in gels (as above) and bands of interest isolated using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio Inc., San Jose, CA). Sanger Direct Sequencing was performed at Azenta Life Sciences (South Plainfield ...
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bioRxiv - Microbiology 2023Quote: ... Human embryonic kidney Lenti-X™ 293T cells (TAKARA, 632180) used for lentiviral packaging and HEK293T cells (ATCC ...
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bioRxiv - Microbiology 2023Quote: Retrovirus plasmids were generated by restriction enzyme cloning of latency genes into pRetroX-IRES-ZsGreen (Takara). Latency locus genes were amplified from WT MHV68-BAC DNA127 and the frame-shift stop mutant ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg of total RNAs were reverse-transcribed into cDNA at 42°C for 1 h in 20 μl reaction mixture containing mouse Moloney leukemia virus reverse transcriptase (PrimeScript, TAKARA BIO, Shiga, Japan) with random 6 primers (TAKARA BIO) ...
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bioRxiv - Molecular Biology 2023Quote: ... with random 6 primers (TAKARA BIO). Thereafter ...
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bioRxiv - Molecular Biology 2023Quote: ... generated cDNA was subjected to real-time PCR analysis using SYBR® Premix Ex Taq™ II kit (TAKARA BIO) with the sets of specific primers (Table 1) ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-dsRed (Clontech/Takara 632496, 1:5000), rabbit anti-MyosinVIIA (Proteus Biosciences 25-6970 ...
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bioRxiv - Genetics 2023Quote: ... Library was prepared following the protocols provided by the manufacturer in SMART-Seq mRNA LP kit (Takara Bio #634768) and Unique Dual Index Kit (U001-U024 ...
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bioRxiv - Developmental Biology 2023Quote: ... pCMV-tdTomato is from Clontech. mCherry-LaminA-C-18 was a gift from M ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-dsRed (Clontech/Takara 632496, 1:5000), rabbit anti-MyosinVIIA (Proteus Biosciences 25-6970 ...
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bioRxiv - Developmental Biology 2023Quote: ... and libraries were prepared as described previously with the following modifications: Libraries were prepared using the SMART-seq v4 Plus/ultra-low RNA input stranded total RNA-seq kit (Takara Bio Inc.) and sequenced on the NextSeq 1000/2000 (100 cycles ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cDNA was synthesized using SMARTScribe Reverse Transcriptase (Takara, CAT# 639537) and PCR-amplified 2.5 mM DNTPs (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... obtained by reverse-transcribing the total RNA extracted from HCT116 cells using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Dalian, China) was used as the template for amplifying the corresponding regions using the PrimeSTAR Max DNA Polymerase (Takara Bio) ...
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bioRxiv - Cancer Biology 2023Quote: ... A Q6 qPCR System was used with TB Green Premix Ex Taq (Takara), and specific pairs of primers (Supplementary Table 3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Three RNA lysis aliquots of the LC6 sample were processed and library preparation completed on the 3 aliquots with SMART-Seq® HT Kit (Takara) and Illumina Nextera reagents for tagmentation ...
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bioRxiv - Cancer Biology 2023Quote: ... was used as the template for amplifying the corresponding regions using the PrimeSTAR Max DNA Polymerase (Takara Bio). The amplicons were then cloned into the HindIII and EcoRI sites of pcEF9-Puro vector (18) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lenti-X GoStix Plus were then used to determine virus titer (Takara Bio cat#631280). For lentiviral infection ...
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bioRxiv - Cancer Biology 2023Quote: ... The pooled media was then filtered using a 0.45 mm filter and concentrated using a Lenti-X™ Concentrator (Takara, #631232). To perform the knockdown ...
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bioRxiv - Cancer Biology 2023Quote: ... doxycycline (Dox) (Takara, 0.2 μg/ml), pan-caspase inhibitor Q-VD-Oph (QVD ...
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bioRxiv - Cancer Biology 2023Quote: ... and oligo dT and random hexamer primers were used together (Takara). A Q6 qPCR System was used with TB Green Premix Ex Taq (Takara) ...
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bioRxiv - Developmental Biology 2023Quote: ... from cDNAs generated with SMARTer Ultra low input RNA kit (Takara Bio) according to manufacturers’ instructions ...
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bioRxiv - Genetics 2023Quote: ... The PCR produc t was purified with a PCR purification kit (Takara Biomedical Technology, Bei jing, China) and was cloned into a pMDTM18-T vector (Takara Biomedical Tec hnology ...
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bioRxiv - Cell Biology 2023Quote: ... mEmerald/ mApple -VTA1: full-length human VTA1 was amplified by PCR and cloned to mEmerald or mApple -C1 vectors (Clontech). mCh-CHMP4C ...
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bioRxiv - Cell Biology 2023Quote: Plasmids NLAD8 HIV-1 AD8 Macrophage-Tropic R5 (NIH AIDS reagents #11346) or HIV Gag-imCherry V3 loop (29) were transfected in HEK 293T cells using CalPhos Mammalian Transfection Kit (Takara Bio) in DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... PCR-mediated site-directed mutagenesis was performed with the pDsRed2-ER mammalian expression vector (Clontech). A BsiW1 restriction site was introduced by substituting adenine for guanine at the 1347th nucleotide (pDsRed2-ER-BsiW1mut ...
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bioRxiv - Developmental Biology 2023Quote: ... and TaKaRa Thermal Cycler Dice (TaKaRa, Japan) as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... Trizol reagent (200 μl; Takara Bio, Kusatsu, Shiga, Japan) was added to the 50 μl worm pellet and subjected to three freeze-thaw cycles in liquid nitrogen with intermittent vortexing for 1 minute to break open the worm bodies ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-DsRed (632496, Clontech), rabbit anti-Lcp1 (Jin et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... and reverse-transcribed into cDNA using Superscript RT Master Mix (Takara, Japan) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... Using TB Green PreMix Ex Tap II (Takara) and specific primers ...
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bioRxiv - Developmental Biology 2023Quote: ... Purified RNAs were then subjected to library generation using SMARTer Stranded Total RNA-Seq Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR was carried out using TB Green Premix Ex Taq II (Takara Bio) and monitored by 7500 Fast Real-Time PCR System ...
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bioRxiv - Evolutionary Biology 2023Quote: We prepared each sequencing library from 1 μg of total RNA using a PrepX PolyA mRNA Isolation Kit (Wafergen, 40047) on the Apollo 324 NGS Library Prep System (Takara Bio USA Holdings, Mountain View, CA). We added Illumina indices and amplified libraries with 12 cycles of PCR and cleaned them using PCRClean DX beads (Aline Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein concentration was quantified using the TaKaRa BCA Protein Assay Kit (TaKaRa, Japan), and 2-mercaptoethanol (Nacalai Tesque ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was performed using the TB Green Premix Ex Taq II (Takara Bio, San Jose, CA, USA) under the following cycling conditions ...
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bioRxiv - Cell Biology 2023Quote: ... using the In-Fusion ® HD Cloning Kit (Z9648N; TaKaRa).
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bioRxiv - Cell Biology 2023Quote: ... Full-length human CLUH and mouse Cluh coding sequences cDNA were cloned in pcDNA3 and pmCherry-N1 (Clontech), respectively ...
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bioRxiv - Cell Biology 2023Quote: 1.0 μM of D/D solubilizer (Takara, Cat: # 635054) in Opti:MEM® (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Gal4-based Matchmaker Two-Hybrid System 3 (Clontech) was used according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli DH5α electrocompetent cells (Takara, Japan, product #9027), adding 2 uL of the product to 100 uL of electrocompetent cells ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli BL21 (DE3) containing a pGro7 plasmid for overexpression of the GroEL/ES chaperone system (Takara Bio, Shiga, Japan). ACP (acyl phosphatase ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli BL21 (DE3) in the presence of GroEL/ES chaperones (pGro7 plasmid, TAKARA Bio, USA). After a pre-screen on agar plates ...
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bioRxiv - Cancer Biology 2023Quote: ... MBP fusion TP63 cassettes were cloned into a lac-inducible MBP-6xHis-TEV bacterial expression vector using In-Fusion cloning system (Takara).