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Citations for Takara Bio :
5901 - 5950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Retroviral supernatant was collected two and three days after transfection of GP2-293T cells and concentrated onto wells of a 6 well plates coated with Retronectin (Takara Bio, 5 ug/ml) by spinning at 2500g for 90 minutes at 32° C ...
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bioRxiv - Cancer Biology 2023Quote: ... then DNA was purified using a PCR cleanup spin column (Takara #74609). The sequencing library preparation was performed using NEBNext Ultra II DNA kit (#E7645S) ...
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bioRxiv - Cancer Biology 2023Quote: ... Three RNA lysis aliquots of the LC6 sample were processed and library preparation completed on the 3 aliquots with SMART-Seq® HT Kit (Takara) and Illumina Nextera reagents for tagmentation ...
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bioRxiv - Cancer Biology 2023Quote: ... obtained by reverse-transcribing the total RNA extracted from HCT116 cells using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Dalian, China) was used as the template for amplifying the corresponding regions using the PrimeSTAR Max DNA Polymerase (Takara Bio) ...
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bioRxiv - Cancer Biology 2023Quote: ... A Q6 qPCR System was used with TB Green Premix Ex Taq (Takara), and specific pairs of primers (Supplementary Table 3 ...
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bioRxiv - Cancer Biology 2023Quote: ... was used as the template for amplifying the corresponding regions using the PrimeSTAR Max DNA Polymerase (Takara Bio). The amplicons were then cloned into the HindIII and EcoRI sites of pcEF9-Puro vector (18) ...
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bioRxiv - Bioengineering 2023Quote: ... 3µg of DNA was digested by EcoRI and the digested DNA was self-ligated using DNA Ligation Kit (Mighty Mix; Takara Bio, Kusatsu, Japan) after purification by ethanol precipitation ...
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bioRxiv - Immunology 2023Quote: ... cDNA library was generated from 2 nanograms of total RNA using Smart-Seq V4 Ultra Low Input RNA Kit (Takara catalog#: 634894). 150 picograms of cDNA was used to make sequencing libraries by Nextera XT DNA Sample Preparation Kit (Illumina catalog# ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by gel purification (Takara Bio Cat. #740609.50). Samples were then pooled and sequenced on a lane of HiSeq 4000 SE50 at the UCSF Center for Advanced Technology (CAT).
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bioRxiv - Cancer Biology 2023Quote: ... 2μL SeqAmp DNA polymerase ((Takara Cat. #638509), 2μL 10uM universal reverse primer (seq ...
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bioRxiv - Cell Biology 2023Quote: A cDNA fragment encoding human FAM53C was isolated by amplifying with nested PCR from human cDNA library plasmid (Takara). The oligonucleotide primer sequences used for the 1st PCR are 5′-CAAAGTGTGCAAGTCAAATCCTGG-3′ (5′ upstream ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared from these samples using the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara) kit according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... to 7.5μL eluted RNA 2.5μL smRNA mix 1 (Takara Cat. #635031) and 1μL 10uM UMI RT primer (seq ...
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bioRxiv - Molecular Biology 2023Quote: ... The SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian (Takara) together with a NovaSeq platform (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: For indexing PCR, 78μL PCR mix (24μL H20, 50μL 2X SeqAmp CB PCR buffer (Takara Cat. #638526), 2μL SeqAmp DNA polymerase ((Takara Cat ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from cells and reverse-transcribed to cDNA using the PrimeScript 1st Strand cDNA Synthesis Kit (TaKaRa Bio). cDNA was used as a template for real-time qPCR analysis on CFX96 Touch (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... 5′-Cy5 and 3′-Cy3 labeled ITS2 RNA was synthesized from Takara Biomedical Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... The antibody used was rabbit polyclonal DsRed (632496; TaKaRa, 1:500).
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bioRxiv - Cell Biology 2023Quote: ... and TB Green Premix Ex Taq II (TaKaRa Bio). In each sample ...
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bioRxiv - Biophysics 2023Quote: Broccoli RNA template sequences were ordered as dsDNA gBlock as above and inserted into a linearized pRZ plasmid using infusion cloning kit (Takara Bio) and transformed into Stellar cells following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... ligated using T4 ligase (Takara) and transformed into chemically competent E ...
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bioRxiv - Biochemistry 2023Quote: ... The virus particles were further concentrated at 10X in volume using Lenti-X-concentrator (Clontech, PT4421-2) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
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bioRxiv - Cell Biology 2023Quote: ... The mutant 2X FLAG TANGO2 Gly154Arg was generated with the In-Fusion HD Cloning Plus Kit (Takara Bio Europe) following the manufacturer’s instructions using the pMK-T plasmid as template ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral vectors for expression of LDHA or LDHB were generated by PCR amplification of the respective cDNAs from plasmids obtained from the Eugene McDermott Center for Human Growth and Development Sequencing Core Human ORF Collection and cloning into pLVX-IRES-Puro (Takara 632183) through Gibson assembly (NEB E2621) ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transfected with each of the above-mentioned vectors and Lenti-X Packaging Single Shots (VSV-G) (Clontech, 631275) in the presence of reduced-serum Opti-MEM medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... fascin-1 subcloned into a pmRFP-C1 expression vector (Clontech), pEGFP-Actin (BD Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was labelled with SYBR Pre-mix Ex Taq (Tli RNase H Plus, Takara) and the Cq values were obtained from the CFX96 TouchTM Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNA was diluted for suitable fold and mixed with qPCR SYBR Green Master Mix (Takara) and primer pairs ...
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bioRxiv - Cell Biology 2023Quote: ... 500 ng total RNA was reversed transcribed into cDNA using the cDNA synthesis kit (Takara). The cDNA was diluted for suitable fold and mixed with qPCR SYBR Green Master Mix (Takara ...
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bioRxiv - Cancer Biology 2023Quote: GP2-293T cells (Takara Bio,631458) were co-transfected with EcoPack packaging plasmid and the MSCV-IRES-GFP (Empty backbone or MycWT or MycMUT ...
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bioRxiv - Cancer Biology 2023Quote: ... GP2 packaging cell line was from Clontech. MDA-MB-231 cells stably expressing Dendra 2 (MDA-MB-231/Dendra2 cell line ...
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bioRxiv - Cancer Biology 2023Quote: ... complementary DNA (cDNA) was synthesized from the total RNA samples using the TaKaRa PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa, 6110A).
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bioRxiv - Cancer Biology 2023Quote: ... The generated lentiviral plasmids were cotransfected with viral packaging plasmids PAX2 and pMD2.G into HEK 293T Lenti-X cells (Clontech) using TransIT-LT1 (Mirus Bio LLC ...
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bioRxiv - Cancer Biology 2023Quote: ... and cloned into the SmaI-linearized pEGFP-C2 vector (Clontech, Catalog No. 632481). Luciferase activity was conducted using a plasmid encoding luciferase under the regulation of the rRNA promoter (provided by L.-L ...
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bioRxiv - Cell Biology 2023Quote: ... concentrated using Lenti-X concentrator (Clontech) according to the manufacturer’s protocol and stored at − 80 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... The Clontech SMART-Seq v4 Ultra Low Input RNA Kit (Takara) was used to generate full-length cDNA ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... pEF1α-TET3G-stable cell lines were then transfected with a pTRE3G-CKAP2:mGL plasmid containing the PTRE3G promoter activatable upon doxycycline exposure and cultured in regular DMEM/F-12 media supplemented with Tet-Approved FBS free of tetracycline contaminants (Takara Bio; 631105). Prior to experiments ...
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bioRxiv - Cell Biology 2023Quote: ... Conventional reverse transcription with random hexanucleotide primers was then performed using the RNA to cDNA EcoDry Premix (639549, TaKaRa) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was then performed on the cDNA using TB Green Premix Ex Taq II (RR820A, Takara), following the instructions manual.
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bioRxiv - Cell Biology 2023Quote: ... was inserted into pEBFP-N1 (Clontech) cut with EcoRI and NotI (so the BFP dropped out) ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription of RNA to cDNA was performed using the PrimeScript RT Reagent kit (RR037A, Takara). Quantitative RT-PCR was then performed on the cDNA using TB Green Premix Ex Taq II (RR820A ...
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bioRxiv - Cell Biology 2023Quote: ... pCDNA3.1 was from Invitrogen while pEGFP-N1 and pQCXIP were obtained from Clontech.
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bioRxiv - Cell Biology 2023Quote: ... pEGFP-N1 (Clontech), pEGFP-C1 (Clontech) ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentrated viruses were titered using the p24-Elisa kit (Takara Bio, cat. 632200) following manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 U/μL RNase inhibitor (Takara), 2.7 x 10-05 AU/μL protease (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: 1.0 U/μl RNase inhibitor (Takara), 2.7 × 10−5 AU/mL protease (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... and pVSV-G (#PT3343-5, Clontech) vectors were transfected using Lipofectamine 3000 ...
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bioRxiv - Cell Biology 2023Quote: ... digested with EcoRI using the In-Fusion HD Cloning Kit (Takara Bio). Then ...
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bioRxiv - Cell Biology 2023Quote: ... as a template and inserted into pEGFP-C2 vector (Takara Bio) digested with EcoRI using the In-Fusion HD Cloning Kit ...