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Citations for Takara Bio :
5851 - 5900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... ORF9c or ORF10 accessory proteins coding sequences (codon-optimized for mammalian expression) were cloned into pLVX-EF1α-IRES-Puro Cloning and Expression Lentivector (Clontech, Takara, #631253) to generate pseudotyped lentiviral particles encoding each accessory protein of SARS-CoV-2 (Wuhan-Hu-1 isolate ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon and FeLV-A/61E provirus digested with SnaB1 and Sph1 (Takara, Kusatsu, Japan) were ligated using In-Fusion HD Cloning Kits according to the manufacturer’s protocol (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... Protoplasts were stained following ApoAlert kit indications (TaKaRa) and the ratio of apoptotic cells was measured by flow cytometry ...
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bioRxiv - Microbiology 2023Quote: ... Then similar protocol was used to isolate total RNA using TRIzol (Takara) reagent according to manufactures’ protocol (Expression Primer list in Supplementary table S-Table 2)
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bioRxiv - Immunology 2023Quote: ... The titer of viral particles was estimated using a Lenti-X p24 Rapid Titer Kit (Clontech) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Full length cDNAs were generated and amplified directly from a single cell through the SMART-Seq HT Kit (Takara Cat. No. 634437). Up to 200pg cDNAs were used to generate the dual index Illumina libraries using Nextera XT DNA Library Prep Kit (Illumina Cat No ...
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bioRxiv - Immunology 2023Quote: ... Full-length cDNA amplification of single cells was performed per the kit protocol (Takara Bio). The analysis of the surface markers of the positive cells was performed on FlowJo.
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bioRxiv - Immunology 2023Quote: ... into 96-well plates containing 12.5μl CDS sorting buffer as described in the SMART-Seq® HT Kit protocol (Takara Bio). Full-length cDNA amplification of single cells was performed per the kit protocol (Takara Bio) ...
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bioRxiv - Genetics 2023Quote: ... electrophoresis was performed using 9 μL of the PCR products on a 2% agarose gel (Takara Bio, Japan) at 100 V ...
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bioRxiv - Genetics 2023Quote: ... The viral solution was further concentrated using the Lenti-X™Concentrator (Takara: 631231) according to the manufacturer’s instructions and stored at -80°C for long-term use ...
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bioRxiv - Immunology 2023Quote: ... 1 μg/ml anti-hCD28 and 10 μg/ml RetroNectin (Takara, Cat# T100A). PBMCs were loaded in these wells in human T cell media (X-VIVO media ...
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bioRxiv - Immunology 2023Quote: ... and 10 μg/ml RetroNectin (Takara, Cat# T100A). Then ...
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bioRxiv - Immunology 2023Quote: ... retronectin-treated plates were prepared by coating with 12.5 μg/ml retronectin (TaKaRa) in PBS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... pCMV-GFP-N1 (Clontech). pEF1-K6a (no tag ...
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Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem functionbioRxiv - Microbiology 2023Quote: ... the 16S rRNA targeted oligonucleotide probes GAM42a (Table S1, TAKARA Bio Inc., Japan) was applied to the identification of γ-Proteobacteria ...
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bioRxiv - Microbiology 2023Quote: ... Site-directed mutagenesis was carried out using Prime STAR Max Premix as described in the manufacturer’s instructions (Takara Bio). All mutations were confirmed by DNA sequencing (Eurofins Genomics).
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Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem functionbioRxiv - Microbiology 2023Quote: ... Negative controls were performed by probe NONEUB (Table S1, TAKARA Bio Inc., Japan).
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bioRxiv - Genomics 2023Quote: ... cells were switched to media made with Tet System Approved FBS (Takara 631367) to reduce background induction ...
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bioRxiv - Genomics 2023Quote: ... and concentrated with Lenti-X concentrator (Takara) following the manufacturer’s manual ...
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bioRxiv - Genomics 2023Quote: ... Lenti-X 293T cells (Takara) are cultured with DMEM medium with 10% of FBS at 37°C and 5% CO2.
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bioRxiv - Genomics 2023Quote: ... The same amount of input RNA for each batch was used for reverse transcription (RT) reactions with SMARTScribe™ Reverse Transcriptase (Takara) and oligo dT following the manufacturer’s manual ...
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bioRxiv - Microbiology 2023Quote: The plasmid constructs used as repair template for recombinant virus generation were generated using InFusion cloning (TaKaRa). The sequence references below are based on the HSV-1 KOS genome accession number JQ673480 (Macdonald et al 2012) ...
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bioRxiv - Immunology 2023Quote: ... added on a retronectin-coated (Takara T100A) non-tissue culture 24-well plate and spun for 90 minutes at 2500 rpm at 10°C ...
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bioRxiv - Genomics 2023Quote: ... the qPCR reactions were carried out using Premix Ex Taq™ (RR390A, TaKaRa) containing 2 μL of cDNA ...
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bioRxiv - Genomics 2023Quote: Five µg of each Quartet RNA sample was reverse transcribed using the PrimeScript™ RT reagent Kit (RR037A, TaKaRa) in a 50 µl reaction ...
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bioRxiv - Immunology 2023Quote: ... pEFTak FLAG-murineMAVS was cloned using InFusion cloning (Takara) of a PCR fragment of the MAVS open reading frame amplified from NIH3T3 cell cDNA and NotI- and PmeI-digested pEFTak FLAG vector ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was generated using the PrimeScript RT-PCR kit (Takara). RT-qPCR was performed using a Viaa7 Real Time PCR instrument (Applied Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Purified lymphocytes were homogenized in RNAiso Plus (Takara Bio Inc., Kusatsu, Japan) and stored at -80 °C until use ...
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bioRxiv - Immunology 2023Quote: ... plasmids were transformed into Stellar cells (Takara Bio), isolated by Maxiprep kits (Macherey Nagel) ...
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bioRxiv - Immunology 2023Quote: ... domain followed by an Avi-Tag(76) and a hexahistidine tag was cloned into a mammalian protein expression vector (pADD2) by In-Fusion (Takara Bio).
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bioRxiv - Immunology 2023Quote: ... and cloned into pLVX-IRES-Puro (Takara) via BamHI and BglII (V5-TurboID ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was isolated from tail biopsies using the NucleoSpin Tissue kit (TaKaRa Bio USA, San Jose, CA) following manufacture protocol ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were performed with PrimSTAR GXL DNA polymerase (TaKaRa Bio USA) with 1 μl of genomic DNA and 0.2 μM primers ...
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bioRxiv - Genomics 2023Quote: ... The second PCR was performed using PrimeSTAR GXL polymerase (Takara) with 5 ng of purified product from the previous PCR was used as a template (50 μL/reaction) ...
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bioRxiv - Immunology 2023Quote: ... All fragments were synthesized as gBlocks (IDT) and subcloned into XhoI/NotI linearized SFG-4αβ by In-fusion cloning (Clontech). To co-express PD-1 with CARs ...
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bioRxiv - Genomics 2023Quote: ... The first PCR was performed using PrimeSTAR GXL polymerase (Takara) and 1:2000 SYBR Green I (20 μL/reaction ...
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bioRxiv - Immunology 2023Quote: 7 μg of lentiviral vectors was mixed with Lenti-XTM packaging single shots (Clontech) in a final volume of 600μl for 15min before the transfection mix were added to 4 million of Lenti-X TM 293T cells seeded on a 10 cm2 tissue culture dish ...
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bioRxiv - Immunology 2023Quote: ... 10 mM dNTPs (all reagents from TaKaRa Biotechnology Co., Ltd., Dalian, China), and 40 pmol of primer mix were added to a 50-μL reaction mixture ...
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bioRxiv - Immunology 2023Quote: ... reverse transcribed using the PrimeScript RT Master Mix (TaKaRa Biomedical Technology Co., Ltd.), and quantified by real-time PCR with TB Green Premix Ex Taq (TaKaRa Biomedical Technology Co. ...
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bioRxiv - Immunology 2023Quote: ... and quantified by real-time PCR with TB Green Premix Ex Taq (TaKaRa Biomedical Technology Co., Ltd.) and ABI 7500 (Applied Biosystems Inc. ...
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bioRxiv - Microbiology 2023Quote: ... we used the following primer sets and In-Fusion Snap Assembly Master Mix (Takara Bio, Shiga, Japan) per the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA was amplified using Premix Ex Taq (Probe qPCR; Takara) in a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using a PrimeScript II first-strand cDNA synthesis kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the RNA samples were treated with recombinant DNase I (TaKaRa). The cDNA was amplified using SYBR Premix Ex Taq II (Tli RNase H Plus ...
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bioRxiv - Microbiology 2023Quote: ... where the In-fusion enzyme (Clontech, USA) fuses PCR-generated sequences containing a 15 bp overlap with linearized vectors on both ends of the insert via homologous recombination.
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bioRxiv - Microbiology 2023Quote: ... the media was replaced and supplemented with 1 μg/mL aTc (Clontech catalog number 631310). After 24 h induction ...
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bioRxiv - Microbiology 2023Quote: ... and the internal control feline peptidyl prolyl isomerase A (PPIA) was amplified using primers Fe-227S and Fe-204R via SYBR Premix Ex Taq II (Tli RNaseH Plus; Takara). Thermal cycling was performed according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... using TransIT®-293 reagent (Takara). Two days later ...
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bioRxiv - Microbiology 2023Quote: ... and MS4 (feline B-cell lymphoma) (73) using an RNAiso Plus kit (Takara), in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA was amplified using SYBR Premix Ex Taq II (Tli RNase H Plus; Takara) in a CFX96 Touch real-time PCR detection system (Bio-Rad ...