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Citations for Takara Bio :
5801 - 5850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... residues 1–1235) were cloned into the mammalian overexpression vector pcDNA3.1 (Clontech) together with a green fluorescent protein and a twin strep-II-tag sequence for affinity purification at the C-terminus ...
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bioRxiv - Genomics 2023Quote: ... and from a male donor (ChiPSC22, Cellartis catalog # Y00320, Takara) were maintained in mTeSR1 medium (STEMCELL Technologies ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... alone or also lacking adenine and histidine (SD-Trp/Leu/Ade/His/AbA) were performed following the manufacturer’s instructions (Clontech).
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bioRxiv - Genetics 2023Quote: ... RNA from 2 retinae of a mouse was extracted using the NucleoSpin® RNA kits (Takara Bio USA, Inc., San Jose, CA). RNA sample concentration and quality was determined with NanoDrop Oneᶜ Microvolume UV-Vis Spectrophotometers (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the SMARTer RNA unique dual index kits (Takara 634452) were used instead of the primers that came with the SMARTer Stranded Total RNA HI Mammalian kit to allow for sequencing on the NovaSeq (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries from treated RNA were created using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNAs was synthesized from total RNA using the PrimeScript™ RT Master Mix (Perfect Real Time, Takara). Quantitative real-time PCR was performed in triplicate using PowerUp SYBR Green (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were stained using rabbit anti-dsRed (1:1000, #632496, Clontech) and mouse nc82 (1:30 ...
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bioRxiv - Plant Biology 2023Quote: ... and introduced into PstI site of the pSXY1033 with In-Fusion HD cloning kit (Takara) to generate transformation vectors pSXY1034 to pSXY1039 (Seq ...
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bioRxiv - Biophysics 2023Quote: All plasmids were constructed using In-Fusion (Takara Bio) cloning and verified via Sanger sequencing (Azenta Life Sciences) ...
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bioRxiv - Cell Biology 2023Quote: ... mAb clone JL-8 (632381) from Takara Bio.
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Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: ... Constructs were ligated (In-Fusion HD, Takara or T4 Ligase, NEB) into pLenti vector behind an EFS promoter using BamHI and NsiI sites ...
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bioRxiv - Molecular Biology 2023Quote: ... The pooled cDNAs were pre-amplified using SeqAmp DNA Polymerase (Clontech, 638509) with the following PCR setup ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of total RNA was used for complementary DNA (cDNA) synthesis by PrimeScript first strand cDNA synthesis kit (TAKARA-Bio, Cat# 6110) using an oligo(dT ...
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bioRxiv - Genetics 2023Quote: ChIP-seq libraries were prepared starting with ∼1000 picograms of ChIP or input DNA samples using the ThruPlex DNA-seq kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using the ThruPLEX DNA-Seq kit (Takara, R400675) and DNA Unique Dual Index Kit (Takara ...
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bioRxiv - Genetics 2023Quote: ... and DNA Unique Dual Index Kit (Takara, R400666). Paired-end sequencing of ChIP-seq libraries was performed (150 bp ...
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bioRxiv - Genetics 2023Quote: ... according to the manufacturer’s instructions (Clontech). Primers used for cloning and point mutagenesis are listed in Table S2.
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bioRxiv - Genetics 2023Quote: ... Amplicons were cloned into the NotI site of the pGL4.23 vector with pSox2 by In-Fusion® cloning (Takara Bio 639650). All plasmids were purified from bacterial culture using Presto™ Midi Plasmid Kit (Geneaid Biotech Inc ...
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bioRxiv - Microbiology 2023Quote: ... or pUC118 vectors (Mighty Cloning Kit; Takara, Kusatsu, Japan). The intact env gene was inserted into the pFUΔss expression vector (51) ...
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bioRxiv - Microbiology 2023Quote: ... were ligated using In-Fusion HD Cloning Kits according to the manufacturer’s protocol (Takara). The resulting chimeras ...
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bioRxiv - Microbiology 2023Quote: ... The Rluc sequence was then replaced by the YFP sequence of pEYFP-N1 (Clontech, Mountainview, CA) by insertion into the NcoI-NotI sites ...
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bioRxiv - Microbiology 2023Quote: ... Amplifying PCR of the first stand cDNA product was then performed using SeqAmp DNA Polymerase (Takara) with a nested 3’ primer to the constant genes and a 5’ universal primer based on universal primer sites added to the 5’ end during cDNA generation ...
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bioRxiv - Microbiology 2023Quote: ... Crude genomic DNA was treated with RNase A (Takara) to remove RNA and purified using the PCR Purification Kit (QIAquick) ...
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bioRxiv - Developmental Biology 2023Quote: ... LA Taq® high fidelity DNA polymerase (TaKaRa, Catalog #RR002A) was used to amplify the CRISPR-Cas9 target site according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... according to manufacturer’s protocols and barcoded (634452, Takara). Urine samples were then sequenced with (either the 2 x 150 bp or 2 x 75 bp configuration ...
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bioRxiv - Genomics 2023Quote: ... a master mix consisted of 150 uL DNA Polymerase (Titanium Taq; Takara), 1 mL of 10x buffer ...
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bioRxiv - Genetics 2023Quote: ... All reactions were carried out to the manufacturer’s recommendations using Ex Taq polymerase (Takara Bio, Shiga, Japan) and primer pairs (Table S2 ...
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bioRxiv - Genetics 2023Quote: First-strand cDNA was synthesized from 5 μg of total RNA using the PrimeScriptTMII cDNA Synthesis kit from Takara (https://takara.com/). The full-length cDNAs of SUH1 and BC10 were amplified using the Taq LA DNA polymerase PCR kit from Takara (https://takara.com/ ...
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bioRxiv - Genetics 2023Quote: ... 200186 and purified by cobalt metal-affinity chromatography (Talon resin, Clontech 635652). cFos/cJun was stored in 18 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was isolated using RNAiso Plus (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Hsf5 cDNA was quantified by DCT method using TB Green Premix Ex Taq II (Tli RNaseH Plus) and Thermal cycler Dice (Takara), and normalized by GAPDH expression level.
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized by SMART-Seq mRNA kit (Takara, 634772). Library DNAs were prepared according to the Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Genomics 2023Quote: All libraries were prepared using the SMARTer Stranded Total RNaseq Kit v2 – Pico Input Mammalian Components (634419, Takara) according to manufacturer’s protocols and barcoded (634452 ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Developmental Biology 2023Quote: ... G9 deletion mutants were generated by PCR amplification or overlap extension PCR and inserted in frame with the MYC tag into the pCS2-MT-NLS expressing vector using the In Fusion Protocol (ST0345, Takara). All constructs were confirmed by Sanger sequencing.
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bioRxiv - Developmental Biology 2023Quote: ... to remove the template plasmid DNA and then self-ligated using Ligation High Ver2 (Takara). All PCR products and enzyme-digested vectors were purified using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... 800 uL of dNTPs (Takara), 50 uL of P5 stagger primer mix (stock at 100 uM concentration) ...
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bioRxiv - Microbiology 2023Quote: ... or In-Fusion HD cloning kit (Takara Bio, San Jose, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA was prepared using the Prime Script RT Reagent Kit (Takara Bio, Shiga, Japan; RR047B) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... coli Stellar cells (Takara Bio) and plated on LB agar supplemented with antibiotics ...
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bioRxiv - Immunology 2023Quote: ... The components of the master mix for the PCR and the PCR programs were set up according to the CloneAmp HIFI PCR protocol (Takara). All generated plasmids were sequenced before use (Eurofins Genomics).
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bioRxiv - Immunology 2023Quote: ... and cloned into the pMIG vector by In-Fusion cloning (ClonTech, Takara). For the design of the cloning primers and plasmids ...
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bioRxiv - Genomics 2023Quote: LentiX HEK293T cells (Clontech, catalogue #632180) were maintained in DMEM high glucose with L-Glutamine and sodium bicarbonate ...
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bioRxiv - Genomics 2023Quote: ... The pB-CAGGS vector and ACE2 insert were ligated by In-Fusion cloning (Takara Bio #638947) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Standard immunoblotting procedures were performed using a primary antibody against GFP (mouse monoclonal JL-8 antibody, Takara) and a secondary antibody goat anti-mouse IgG-peroxidase antibody (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... and isolated by NucleoSpin Plasmid (TaKaRa). The introduced mutation was verified by Sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio, catalog no. R045B) per the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... Samples were processed using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.). Briefly ...
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bioRxiv - Immunology 2023Quote: ... ORF9c or ORF10 accessory proteins coding sequences (codon-optimized for mammalian expression) were cloned into pLVX-EF1α-IRES-Puro Cloning and Expression Lentivector (Clontech, Takara, #631253) to generate pseudotyped lentiviral particles encoding each accessory protein of SARS-CoV-2 (Wuhan-Hu-1 isolate ...