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Citations for Takara Bio :
5751 - 5800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... T-2A-H2B-tdTomato was generated by replacing the EGFP fragment with H2B-tdTomato using the In-Fusion® HD Cloning Kit (Catalog #639648, Takara, Kusatsu, Japan), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... KhES-1 was cultured at feeder-free conditions using StemFit® AK02N (Catalog #RCAK02N, TAKARA BIO, Kusatsu, Japan) with daily medium change ...
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bioRxiv - Bioengineering 2023Quote: ... Laminin 511 E8 fragment (#T304, TAKARA BIO, Kusatsu, Japan) (2.5 µg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was reverse transcripted from 500ng RNA using PrimeScript™ II 1st strand cDNA Synthesis Kit (Catalog # 6210A TAKARA BIO, Kusatsu, Japan) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... subtilis 168 and the chloramphenicol resistance gene was amplified from plasmid pDG1662 according to the manufacturer’s instruction (Takara Primestar max DNA polymerase). These three fragments were combined by overlapping Polymerase Chain Reaction (PCR ...
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bioRxiv - Neuroscience 2023Quote: ... Insoluble material was removed by centrifugation and the supernatant containing the receptor was rotated with Talon resin (Takara) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of the total RNA was used for cDNA synthesis using the Prime Script 1st Strand cDNA Synthesis Kit (TaKaRa, 6110A). Random 6-mer primers (0.3 µL ...
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bioRxiv - Molecular Biology 2023Quote: The commercially available pEGFP-C2 (Clontech) plasmid was used as a sense EGFP construct ...
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bioRxiv - Microbiology 2023Quote: ... SEAP activity was quantified using the Great EscAPe SEAP assay (Clontech). Fluorescence was read on a Filtermax F5 plate reader (Molecular Devices).
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bioRxiv - Biophysics 2023Quote: ... by In-Fusion ligation (Takara Bio) and transformed into Rosetta 2 E ...
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bioRxiv - Neuroscience 2023Quote: ... Arr3 and Arr3-derived constructs were detected with rabbit anti-Arr3 (Ahmed et al., 2007) or mouse anti-GFP (Clontech JL-8) antibody ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNAs were isolated with SimplePrep reagent (TaKaRa Bio). PCR was performed using KOD FX neo (Toyobo ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and supernatants were incubated with cobalt resin (Takara) for 1 hour at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the synthesized cDNA was used to check the expression of the GFP transcript by semiquantitative PCR using GTPCR master mix (TAKARA BIO) for the GFP-specific primer ...
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bioRxiv - Neuroscience 2023Quote: ... and 25 μl PrimeSTAR Max PCR Readymix (Takara, Mountain View, CA, USA). PCR reactions were carried out by 30 cycles of denaturation at 98°C for 10 seconds ...
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bioRxiv - Neuroscience 2023Quote: ... Subcloning was carried out using the In-Fusion cloning kit (Takara Bio). Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5U/μL RNAse inhibitor (Takara, 2313A), 0.5% biocytin and 0.02 Alexa 488 ...
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bioRxiv - Neuroscience 2023Quote: ... cDNAs were prepared with Clontech Smart-seq v4 ultra-low kit (Cat# 634888, Takara Bio, Shiga, Japan). PolyA tail enrichment was done using NEBNext® Poly(A ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA segments coding MIR319A and MIR319B loci (with genomic DNA as the template) were amplified using PrimeSTAR HS DNA polymerase (TaKaRa Bio, Kusatsu, Japan) with miR319aF-miR319R or miR319bF-miR319R primer pairs and were sequenced according to Sanger method ...
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bioRxiv - Neuroscience 2023Quote: ... Constructs containing point-mutated ARE-like sequences were prepared using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Because an ARE half-site can function alone to confer androgen inducibility (Pihlajamaa et al. ...
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bioRxiv - Neuroscience 2023Quote: ... or control mCherry were subcloned using the InFusion HD cloning kit (Takara Bio USA). Furthermore ...
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bioRxiv - Pathology 2023Quote: ... cDNA was prepared using PrimeScript cDNA synthesis kit (Takara, Cat No: 2680A) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... and RNA was isolated using TRIzol (Takara, Japan). The quality and quantity of RNA were analyzed using NanoDrop2000 (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... AAV stock concentration was determined using the Takara AAV Titration kit (Takara #6233) and an Applied Biosystems 7500 Fast Real-Time PCR System.
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bioRxiv - Neuroscience 2023Quote: ... 2 μg/ml doxycycline (Clontech, cat. no. 631311), 1X N2 supplement (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... 2 μg/ml doxycycline (Clontech, cat. no. 631311), 1X N2 supplement (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... 7 million Lenti-X HEK-293T cells (Takara Bio, cat. no. 632180) were seeded in 9 mL DMEM (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 6 ml Lenti-X Concentrator (Takara Bio; Cat. No. 631231), mixed thoroughly ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was prepared from the extracted RNAs with oligo (dT) and random primers using the PrimeScript RT reagent kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the open reading frames (ORFs) of genes and their mutant alleles were amplified and cloned into the pGADT7 prey or pGBKT7 bait vector (TaKaRa Bio, Tokyo, Japan). The resulting bait and prey constructs were transformed in pairs into yeast strain AH109 and assayed for growth on SD-His-Leu-Trp plates ...
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bioRxiv - Molecular Biology 2023Quote: ... and the NucleoSpin RNA Virus was from Takara (cat. 740956)
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bioRxiv - Molecular Biology 2023Quote: ... 12.5 U/μl Smartscribe reverse transcriptase (639538, Takara), 2.5 mM dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: ... we enriched the lentiviral particles with Lent-X Concentrator (Cat# 631231, Clontech, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Lentivirus was produced using a standard protocol64 from a 15 cm plate of 25M lenti-x 293T cells (Clontech #632180) via polyethylenimine (PEI ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) using the RR036A PrimeScript™ RT Reagent Kit (Perfect Real Time) supplied by TaKaRa. The amplification products were quantified using SYBR Green (Vazyme) ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative titer was estimated by Lenti-X GoStix Plus (Takara #631280) and was similar for all viral constructs.
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bioRxiv - Immunology 2023Quote: ... The virus titer was determined using Lenti-XTM GoStix Plus (Clontech).
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative RT-PCR for pluripotency and trilineage spontaneous differentiation was performed according to the instruction manual of the human ES cell Primer Array (Takara Clontech).
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bioRxiv - Plant Biology 2023Quote: ... paired-ended libraries were constructed using SMART ChIPseq kit (TAKARA) and sequenced using HiseqX ...
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bioRxiv - Physiology 2023Quote: ... PCR products were cleaned with NucleoMag beads (Takara Bio, Catalog #744503) and then visualized using gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... MtU2AF35b and RiSP7 or MtSR45 truncation constructs were amplified with restriction sites and subsequently cloned into the pGBKT7 or pGADT7-REC vectors (Takara Clontech Bio Europe ...
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bioRxiv - Plant Biology 2023Quote: ... the correct full-length sequences were determined for the laboratory strains using a SMARTer RACE 5’/3’ kit (Takara, Shiga, Japan). These sequences were aligned by ClustalW ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and deposited into a PCR tube with 5 μL lysis buffer (Takara Bio, San Francisco, CA). cDNAs were then prepared by reverse transcriptase (Takara Bio ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cDNAs were then prepared by reverse transcriptase (Takara Bio, San Francisco, CA) and the antibody coding regions were amplified by PCR ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the protein concentration was assessed and quantified using BCA (TaKaRa, Japanese). Immunoblotting was conducted on cell and tissue extracts (30 μg total protein/well ...
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bioRxiv - Plant Biology 2023Quote: ... coli stellar cells (Takara, JP) were used for transformation by the heat shock method with well-established protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Co-purified cellular RNAs were degraded by incubating with 100U micrococcal nuclease (Clontech, 2910A) on-resin in 25 mL of Nickel wash buffer supplemented with 5 mM CaCl2 at room temperature for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... ds-cDNA was generated using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...