Labshake search
Citations for Takara Bio :
5701 - 5750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: The HEK293T lentivirus packaging (Clontech, USA) and 293Vec-RD114 retrovirus packaging cell lines (BioVec Pharma ...
-
bioRxiv - Molecular Biology 2023Quote: ... A synthetic anti-CD45 scFv gene (GeneCust, France) was cloned and inserted into the pLV2 lentiviral vector (Clontech, USA) and pMM25b retroviral vector (a gift from Dr ...
-
bioRxiv - Molecular Biology 2023Quote: ... or an empty vector control (pRK5-Myc, Clontech, USA), as well as the PPARγ target gene luciferase reporter plasmid (PPRE ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV2/9 particles were purified using the AAV Purification kit (Takara, Siga, Japan). The AAV solution was concentrated to the optimal volume by centrifugation using an Amicon Ultra-4 100k centrifugal filter unit (Millipore) ...
-
bioRxiv - Plant Biology 2023Quote: ... The amplified promoter and linearised pMDC32 vector were ligated using the infusion cloning enzyme from Takara Bio ...
-
bioRxiv - Physiology 2023Quote: For Matchmaker Gold yeast one-hybrid system (Clontech, Mountain View, CA, USA), the MaMYB4 CDS was fused to the GAL4 transcription factor activation domain (GAL4AD ...
-
bioRxiv - Plant Biology 2023Quote: ... The fragments of PtBST1 and PtBST2 were cloned into the EcoRI restriction site in pPha-T1/egfp vector (Tachibana et al., 2011) using Mighty Mix (Takara). PtBST3 and PtBST4 fragments were cloned using Gibson assembly system (New England Biolabs ...
-
bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed with GeneAce SYBR qPCR Mix α No ROX (Nippon Gene, Tokyo, Japan) in Thermal Cycler Dice Real Time System II (Takara). Each Actin1 genes (PtActin1 and TpActin1 ...
-
bioRxiv - Plant Biology 2023Quote: ... Yeast-two-hybrid library screens were carried out according to Matchmaker® Gold Yeast Two-Hybrid System User Manual (Takara Clontech Bio Europe, PT4084-1) using a cDNA library of M ...
-
bioRxiv - Plant Biology 2023Quote: ... Yeast-two-hybrid library screens were carried out according to Matchmaker® Gold Yeast Two-Hybrid System User Manual (Takara Clontech Bio Europe, PT4084-1) using a cDNA library of M ...
-
bioRxiv - Plant Biology 2023Quote: ... HRP substrates (Takara) and an iBrightTM CL750 Imaging System were used for immunodetection.
-
bioRxiv - Neuroscience 2023Quote: ... They were then incubated overnight at 4°C with the following primary antibodies diluted in PBS - 0.3% Triton X100 - 1% NGS: rabbit anti-ZsGreen (1/1000, Clontech, 632474, RRID:AB_2491179) or rabbit anti- DCX (1/2000 ...
-
bioRxiv - Neuroscience 2023Quote: ... Isolated RNA was dissolved in 10 μl of DEPC-treated water and reverse-transcribed using a reverse transcription reagent kit (PrimeScript RT Reagent Kit with gDNA Eraser, Takara) and a thermal cycler (Mastercycler ...
-
bioRxiv - Neuroscience 2023Quote: ... and purified cDNA was eluted in 17 μl elution buffer provided by Takara. All samples were quantitated using PicoGreen® on Molecular Dynamics M2 SpectraMax instrument ...
-
bioRxiv - Neuroscience 2023Quote: ... rabbit anti-DsRed (Clontech), guinea-pig anti VGLUT1 (Synaptic Systems ...
-
bioRxiv - Neuroscience 2023Quote: ... The resultant cDNA was amplified by PrimeSTAR (Takara) with cycles of 95°C 10 s ...
-
bioRxiv - Neuroscience 2023Quote: ... cDNAs were prepared with SmarSeq Ultra Low input kit (Takara).
-
bioRxiv - Neuroscience 2023Quote: ... Total RNA was heat denatured and digested with 10-20 U of MazF enzyme (TakaRa, ref. 2415A) for 15 min at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... The resulting PCR product was cloned between the 3’ and 5’ arms of the Rosa26 targeting vector using In-Fusion cloning (Takara Bio). Plasmid sequence was checked by sequencing ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were sectioned into 1000-μm coronal slices under microscopy and the sections were floated in 1% BSA-PBS (Nacalai Tesque, #0128197 and Takara, #T9181). The PVH was dissected based on the fluorescence of tdTomato in OT-Cre ...
-
bioRxiv - Neuroscience 2023Quote: ... mRNA amplification and cDNA preparation were performed using the SMARTer mRNA amplification kit (Takara #634940). Labeled samples were sequenced using an Illumina NextSeq 500 sequencer using 75 base pair single read protocols.
-
bioRxiv - Neuroscience 2023Quote: pcDNA-PCDH19-Gal4DBD-VP16 was created by In-Fusion cloning (Takara Bio) using PCDH19-FL and Gal4DBD-VP16 PCR products and a pcDNA vector digested with BamHI and EcoRI ...
-
Brief Change in Dopamine Activity during Consolidation Impairs Long-Term Memory via Sleep DisruptionbioRxiv - Neuroscience 2023Quote: ... and rabbit anti-DsRed (1:200, Cat# 632496, Clontech). Secondary antibodies were Alexa Fluor 488 (goat anti-chicken ...
-
Brief Change in Dopamine Activity during Consolidation Impairs Long-Term Memory via Sleep DisruptionbioRxiv - Neuroscience 2023Quote: ... and rabbit anti-DsRed (1:200, Cat# 632496, Clontech); primary antibodies used to detect GRASP signals was mouse monoclonal anti-GFP (1:200 ...
-
bioRxiv - Neuroscience 2023Quote: ... Viral titre was determined by qPCR (LentiX, Takara).
-
bioRxiv - Neuroscience 2023Quote: ... Plasmid DNA used to generate lentiviral particles were transfected into HEK293 cells using LentiX single-shot VSV-G (Takara) following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... anti-GFP (Clontech, JL-8, 1:10,000 in 5% non-fat milk) and anti-GAPDH (Millipore ...
-
bioRxiv - Neuroscience 2023Quote: ... in PBS and then incubated overnight with gentle agitation at 4 °C in blocking solution plus 1:1000 dilution primary antibody (chicken anti-GFP polyclonal, Abcam; rabbit anti-dsRed polyclonal, Takara Bio). Sections were then incubated covered with gentle agitation for 2 hours at room temperature in blocking solution plus 1:500 dilution secondary antibody (goat anti-rabbit IgG Alexafluor 594 conjugate ...
-
bioRxiv - Neuroscience 2023Quote: ... The α-synA53T–YFP construct was subcloned into the pIRESpuro3 vector (Clontech; Takara Bio, JPN) using NheI (5′ ...
-
bioRxiv - Neuroscience 2023Quote: ... the RNA was processed using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara, #634411). The fragment length of the libraries was verified using the Fragment Analyzer 12 (Agilent) ...
-
bioRxiv - Neuroscience 2023Quote: ... We then synthesized these sequences as G-blocks (Integrated DNA Technologies) and cloned them using Infusion kit (Takara biosciences catalog # 638948) into pSMART-HC-Kan (Lucigen ...
-
bioRxiv - Neuroscience 2023Quote: Total RNA was extracted using RNAiso Plus (9108, Takara) based on the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... The α-synA53T–YFP construct was subcloned into the pIRESpuro3 vector (Clontech; Takara Bio, JPN) using NheI (5′ ...
-
bioRxiv - Neuroscience 2023Quote: ... Rabbit primary anti-RFP (anti-DsRed Living Colors, 632496, Takara, Mountain View, CA, 1:1000) was followed with donkey anti-rabbit AF594 (715-586-152 ...
-
bioRxiv - Neuroscience 2023Quote: ... filtered through 0.45 μm syringe filter set and concentrated using Lenti-X concentrator (Takara Bio, 631231). The virus titer was measured using Lenti-X GoStix Plus assay kit (Takara Bio ...
-
bioRxiv - Neuroscience 2023Quote: ... The virus titer was measured using Lenti-X GoStix Plus assay kit (Takara Bio, 631280).
-
bioRxiv - Molecular Biology 2023Quote: ... and libraries were constructed using the SmarterStranded V2 kit (Takara Bio). Paired-end (PE ...
-
bioRxiv - Neuroscience 2023Quote: ... CDNA was reverse-transcribed using PrimeScript™ RT reagent Kit (Takara). Real-time quantitative PCR was performed using QuantiNova® SYBR® Green RT-PCR Kit (Qiagen ...
-
bioRxiv - Neuroscience 2023Quote: ... and the target bands at 14,438 bp were cut and purified with NucleoSpin® Gel and PCR Clean-Up kit (740609.250, Takara). The target amplicons were re-concentrated to > 300 ng/ µL with GlycoBlue™ Coprecipitant (AM9515 ...
-
bioRxiv - Neuroscience 2023Quote: scRNA-seq was performed using the SMART-Seq v4 kit (Takara Cat#634894) as described previously (Tasic et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... 48-52 mCherry-positive cells from each mouse were sorted into 8-well strips containing SMART-Seq lysis buffer with RNase inhibitor (0.17 U/µL; Takara Cat#ST0764). For retrograde transsynaptic tracing ...
-
bioRxiv - Plant Biology 2023Quote: ... The reactions were carried out using TB Green® Premix Ex Taq™ II (TAKARA-Bio, Cat no. RR82WR) and incubated at 95 °C for 2 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... for plasmid library purification with NucleoBond® Xtra Midi EF kit (740420.10, Takara).
-
bioRxiv - Plant Biology 2023Quote: ... Polymerase chain reaction (PCR) was carried out using PrimeSTAR® GXL DNA Polymerase (TAKARA-Bio, Cat# R050). The PCR conditions were specific to each sequence and amplified using 10 μM sequence-specific paired oligonucleotides (see Supplementary Table 5 ...
-
bioRxiv - Neuroscience 2023Quote: ... High-titer lentivirus was produced by concentrating the filtered supernatant with Lenti-X concentrator (Takara, 631231) and stored at -80 °C in aliquots.
-
bioRxiv - Microbiology 2023Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
-
bioRxiv - Molecular Biology 2023Quote: HEK293T/17 cells were co-transfected with 0.8 pmol SINEUP-GFP or miniSINEUP-GFP FRAM (0.8 pmol) and 0.2 pmol sense pEGFP-C2 plasmids (Clontech) in each well of a 6-well culture plate ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 µL of RNaseH (TaKaRa, 2150A), and 1 µL of RNase cocktail (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded onto TALON metal affinity resin (Takara Bio) pre-equilibrated with TBS pH 8.0 containing 10 mM imidazole ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded onto 100 μL TALON metal affinity resin (Takara Bio) pre-equilibrated with TBS pH 8.0 containing 10 mM imidazole ...