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Citations for Takara Bio :
5651 - 5700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293T-LentiX cells (Takara Biosciences) were transfected with 750 ng of an equimolar mixture of the three third-generation packaging plasmids (pMD2.G ...
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bioRxiv - Synthetic Biology 2023Quote: ... for Infusion Cloning (Takara Bio). Plasmids were transformed into chemically competent NEB Turbo E ...
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bioRxiv - Synthetic Biology 2023Quote: Human Embryonic Kidney (HEK) 293 cells (ATCC, CRL-1573) and HEK293T-LentiX (Takara Biosciences) were cultured in standard culture conditions (37ºC and 5% CO2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Rapalog AP21967 (also known as A/C heterodimerizer, purchased from Takara Biosciences; catalog# 635056) was administered at the time of transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... A second 23-25 cycles PCR was performed with PrimeStar HS polymerase (Takara) or KAPA HiFi DNA Polymerase (Roche) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and pCMV-Tet3G Vector (Takara # 631335). Instead ...
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bioRxiv - Synthetic Biology 2023Quote: ... pTRE3G and rtTA3G sequences were amplified from pTRE3G-BI-ZsGreen1 (Takara #631339) and pCMV-Tet3G Vector (Takara # 631335) ...
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bioRxiv - Cancer Biology 2023Quote: ... the final construct was assembled into the Adeno-X adenoviral system (Takara Bio) via In-Fusion cloning as described by manufacturer protocol.
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bioRxiv - Cancer Biology 2023Quote: Sequencing libraries were generated by SMARTer Stranded Total RNAseq Kit – Pico Input Mammalian V3 (Takara Bio, #634485) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were incubated at 3×104 cells/well in 96-well U bottom plates for 24 hours in T cell media containing 50 IU/mL rhIL-2 and varying concentrations of AP20187 (B/B homodimerizer, Takara, 635058). After 24 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... Stellar™ Competent Cells (Takara) were used for transformation ...
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bioRxiv - Microbiology 2023Quote: ... using 3000 U of XbaI (Takara, Japan) following the PulseNet website protocols (https://pulsenetinternational.org/protocols/pfge/) ...
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bioRxiv - Molecular Biology 2023Quote: Lenti-X HEK293T cells (Takara Bio) were seeded at a density of 4.5×106 cells per 10 cm dish 18-24 h prior to transfection ...
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bioRxiv - Microbiology 2023Quote: ... the CPER products (25 μl) were transfected into VeroE6/TMPRSS2 cells using TransIT-X2 Dynamic Delivery System (Takara, Cat# MIR6003) according to our previous report22 ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.59) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 h posttransfection) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4x Lenti-X concentrator reagent (TaKaRa; 631231) was added to the lentivirus-containing supernatant at a final concentration of 1x and incubated at 4C for 1hr ...
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bioRxiv - Synthetic Biology 2023Quote: ... PrimeSTAR HS DNA Polymerase was purchased from Takara Bio (Kusatsu ...
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bioRxiv - Synthetic Biology 2023Quote: A 10 cm dish of HEK293T cells that had been transfected with the GFP expressing plasmid pEGFP-N1 (Clontech) was washed with PBS and lysed by sonication on ice in PBS supplemented with 0.2 % Triton X100 ...
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bioRxiv - Cancer Biology 2023Quote: ... His-TEV tagged protein was captured with Co-TALON resin (Clonetech, Takara Bio USA, 2 mL slurry/liter culture) at 4 ºC for 1 h with constant end-to-end mixing ...
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bioRxiv - Synthetic Biology 2023Quote: Polymerase chain reaction (PCR) amplification of DNA receivers for plasmids construction and colony PCR were performed using PrimeSTAR® Max DNA Polymerase (R045B, TaKaRa, Kyoto, Japan). PCR primers were synthesized by Integrated DNA Technologies (IDT ...
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bioRxiv - Synthetic Biology 2023Quote: ... SEAP activity in supernatant (25 μL) was measured using the Great EscAPe SEAP Chemiluminescence Assay kit 2.0 (Clontech, Fremonet, CA). The GLuc activity in supernatant (20 μL ...
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bioRxiv - Pathology 2023Quote: ... with SYBR Premix Ex TaqTM II (TaKaRa, Japan). The RT-qPCR was performed for at least three biological replicates under the following conditions ...
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bioRxiv - Neuroscience 2023Quote: ... Pelleted nuclei were then washed in 4 mL of Nuclei Suspension Buffer containing 0.2% RNase inhibitor (Clontech/Takara, Cat. 2313B) and centrifuged at 500xg for five minutes at 4°C ...
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bioRxiv - Pathology 2023Quote: ... and reverse transcribed using PrimeScript RT Master Mix for RT-PCR (Takara Bio, Japan). Real-time PCR was performed using SYBR Select Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized with the PrimeScript 1st Strand kit with an additional primer mix containing random DTs (#RR047A and #6110A, Takara). cDNAs were amplified using specific Taqman probes ...
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bioRxiv - Pathology 2023Quote: RNA from tissues was isolated using TRIzol reagent (Takara Bio, Japan) and reverse transcribed using PrimeScript RT Master Mix for RT-PCR (Takara Bio ...
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bioRxiv - Pathology 2023Quote: Total RNA was extracted from skin biopsies and cell lysates using RNAiso Plus Reagent (Takara, Cat No: 9109) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... by In-Fusion cloning (Clontech). The resulting vector was further used for transfection with BD BaculoGold (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... the eYFP encoding cDNA plasmid (Clontech) was precipitated onto colloidal Au particles (1.6µm ...
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bioRxiv - Neuroscience 2023Quote: ... The expression of GFP- or Venus-tagged constructs was measured by Western blot with mouse anti-GFP antibody (Clontech; 1:2,000). The expression of WT and mutant arrestin-3 was measured with rabbit polyclonal anti-arrestin-3 antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Two micrograms of the RNA were subjected to reverse transcription with random hexamers and the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa). For semi-quantitative PCR ...
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bioRxiv - Neuroscience 2023Quote: ... a mix of an mCherry-DsRed rabbit polyclonal antibody (Living Colors-Clontech Antibody 632496, 1:1000) and a mouse monoclonal antibody against oxytocin (P38 ...
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bioRxiv - Neuroscience 2023Quote: ... the mouse monoclonal anti-GFP (Jl-8, Clontech; 1:500 overnight at 4°C) primary antibody was used ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted using Nucleospin RNA XS (TaKaRa, Tokyo, Japan) or RNAiso Plus reagent (TaKaRa) ...
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bioRxiv - Neuroscience 2023Quote: ... or RNAiso Plus reagent (TaKaRa), followed by the removal of residual DNA with the Turbo DNA-free kit (Ambion ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was prepared from total RNA using the PrimeScript RT-PCR kit (Takara, #RR037Q). Relative gene expression of the cDNA was assayed using a 7500 Fast real-time PCR instrument (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... total RNAs were reverse transcribed to complementary DNA (cDNA) using both oligo dT and random primers with PrimeScript RT Reagent Kit (Takara, #RR037A) according to manufacturer’s instructions in the presence of a synthetic external non-homologous poly(A ...
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bioRxiv - Molecular Biology 2023Quote: ... The solution was treated with 5 μg/mL RNaseA and 70 unit/ml DNase I (Takara) for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 μl of cold Poly-A-tailing master mix (3.3x Smartscribe first strand buffer (Takara), 0.16 U/μl Poly-A-polymerase (2180A ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized using RNA to cDNA EcoDry Premix (Takara, 639547). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Biorad ...
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bioRxiv - Molecular Biology 2023Quote: ... or XFect Transfection Reagent (Takara, 631318) with two px458 (Addgene ...
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bioRxiv - Molecular Biology 2023Quote: ... The insert was cloned into the pAM_1C vector using the In-Fusion snap assembly master mix according to manufacturer’s instructions (Takara Bio, Kyoto, Shimogyō-ku, Japan). The purified plasmid was verified by Sanger sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... or an empty vector control (pRK5-Myc, Clontech, USA). Cells were briefly fixed with 1:1 dilution of methanol and acetone at -20 °C and rinsed with TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... and then the olfactory and brain tissues were dissected and pooled from 20-25 mosquitoes in Trizol(73) (RNAiso Plus, Takara Bio, Kusatsu, Japan) for a total of three biological replicates ...
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bioRxiv - Molecular Biology 2023Quote: We extracted total RNA from rat cells and tissues employing the TRIzol reagent (D9108A, TaKaRa Bio). The RNA was then reverse-transcribed into complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... and axis sites (YBP1, GRR1) (see qPCR Primer Table) using the TB Green Premix Ex Taq II (Tli RNAse H Plus, Takara).
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from the above total extracted RNA as a template using a PrimeScript First-Strand cDNA Synthesis Kit (Takara Bio, Beijing, China). Specific primers (Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... were collected in Trizol (RNAiso Plus, Takara Bio, Kusatsu, Japan) for RNA extraction and cDNA preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... pHLP735 and pHLP747 were constructed using the In-Fusion cloning system (Takara Bio). The RTS1 gene ...
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bioRxiv - Molecular Biology 2023Quote: ... The lentivirus packaging HEK293T cell line was purchased from Clontech, USA ...