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Citations for Takara Bio :
5601 - 5650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Pyroptosis was induced by addition of 500nM B/B-Homodimerizer (Takara Bio, AP20187) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Fifty cells were collected in a tube containing ice-cold lysis buffer and processed for cDNA synthesis according to the Smart-Seq v4 manual (Takara Bio, 634890) with a PCR cycle number of 11 ...
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bioRxiv - Microbiology 2023Quote: ... 2.5 mM dNTPs (Takara Bio USA, Madison, WI), 2 mM of each primer (final concentrations) ...
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bioRxiv - Microbiology 2023Quote: ... and mRNA reverse-transcribed into cDNA using the PrimeScript cDNA Synthesis Kit (TaKaRa). qRT-PCR was set up according to the manufacturer’s instructions (Alkali Scientific ...
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bioRxiv - Microbiology 2023Quote: ... utilizing the plasmid pPTR I (TAKARA, Japan) as a template and the primer pairs pHSG396-ptrA-IF-F and pHSG396-ptrA-IF-R ...
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bioRxiv - Immunology 2023Quote: ... M-IgA was expressed by transfecting a pair of alpha heavy and kappa light chain genes into FreeStyle CHO-S cells that were grown in a 24-well plate according to the manufacturer’s protocol (CHOgro High Yield Expression System, Takara Bio, Shiga, Japan). For large-scale antibody production ...
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bioRxiv - Cell Biology 2023Quote: For the synthesis of cDNA with random hexamers (PrimeScript RT reagent kit from Takara Bio) 300 ng of total RNA were used ...
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bioRxiv - Cancer Biology 2023Quote: ... and mouse anti-SPARC/Osteonectin (ON1-1, Takara) for 1 h at RT ...
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bioRxiv - Genetics 2023Quote: Full-length mouse Rnf212b and Rnf212 cDNAs were amplified by PCR from mouse testis cDNA prepared as above and cloned into pGADT7 and pGBKT7 vectors (Clontech) using the Gibson Assembly Cloning Kit (NEB ...
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bioRxiv - Genetics 2023Quote: ... The libraries for Illumina sequencing were constructed using the ThruPLEX DNA-Seq Kit (Clontech) and purified using SPRIselect beads (Beckman Coulter) ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Genomics 2023Quote: ... We extracted high-molecular-weight (HMW) DNA using Nucleobond® HMW DNA extraction kit (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... gDNA was amplified using CloneAmp™ HiFi PCR Premix (Takara 639298) and dmd Sanger sequencing primers ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... using in-fusion cloning kit (Takara, Kusatsu, Japan). To generate pFA6a-link-GBP-CaURA3 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using a Thermal Cycler Dice Real Time System II (Takara Bio Inc., Otsu, Japan) and SYBR Premix Ex Taq II (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... Ribosomal RNA was removed using RiboGone – Mammalian Kit protocol (Clontech, No. 634847). After rRNA depletion ...
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bioRxiv - Cell Biology 2023Quote: The pCAG-MDM4 expression vector was established by cloning of MDM4 sequence into a pCAG vector47 containing a multiple cloning site (pCAG-MCS) using In-Fusion® Snap Assembly Starter Bundle kit (#638945; Takara Bio). A single restriction digest was performed on the pCAG-MCS vector using XhoI (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Rapalog A/C hetero-dimerizer (635057, Takara), Bafilomycin A1 (sc-201550 ...
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: ... qPCR was performed using SYBR Green PCR Master Mix (Takara, Cat.# RR820) on a StepOnePlus system (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed q-RTPCR using TB Green Premix Ex Taq Ⅱ (Takara, Japan) with specific primers (Supplemental Table 1) ...
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bioRxiv - Cell Biology 2023Quote: Human bone marrow mesenchymal stem cells (BM MSCs) (TaKaRa Bio) were cultured in Mesenchymal Stem Cell Growth Medium 2 (TaKaRa Bio ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T-LentiX (Takara Bio, cat.no. 632180) and HeLa (Unauthenticated HeLa-Kyoto ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... the Tet-On® 3G Inducible Expression System kit was used (Takara Bio; 631167). RPE-1 wild-type and CKAP2-KO cells were transfected with a pEF1α-TET3G plasmid for expression of a Tet-On 3G transactivator protein ...
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bioRxiv - Cancer Biology 2023Quote: ... with the exception that 1U/μL of RNAse inhibitor (Takara Bio, #2313A) was added to all buffers during and after nuclei isolation (i.e ...
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bioRxiv - Cancer Biology 2023Quote: ... above 20% were used for downstream library generation using the SMARTer Stranded Total RNA – Pico RNA-seq kit v2 (Takara). Libraries were sequenced using 100 nucleotide paired-end sequencing on a NovaSeq 6000 (Illumina).
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bioRxiv - Cancer Biology 2023Quote: ... Clarified lysate was mixed with ∼0.5 ml/L of culture cobalt resin (TaKaRa) for one hour ...
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bioRxiv - Cancer Biology 2023Quote: ... TP63 cDNA constructs were cloned into a lentiviral IRES-Neo vector using the In-Fusion cloning system (Takara). A 3×FLAG tag was added to TP63 at the N- or C-terminus ...
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bioRxiv - Neuroscience 2023Quote: ... and SHANK3-3D (T551D/S694D/S781D) were generated with a PrimeSTAR Mutagenesis Basal Kit (Takara, Shiga, Japan). pGEX-14-3-3ζ ...
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bioRxiv - Neuroscience 2023Quote: ... and shLGR5 was cloned into the pSiren-RetroQ-ZsGreen vector (Clontech). For lentivirus preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.16 U/μl Poly-A-polymerase (2180A, Takara), 3.3 U/μl SuperaseIn RNase inhibitor (AM 2694 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10-μL TB Green Premix Ex Taq II (Takara, Beijing, China), and 7-μL sterile deionized water ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloned into pMD19-T vector (Takara Bio, Dalian, China) before transformation into E ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was added to the Ni-IDA resin (Takara, Cat # 635660) equilibrated with Binding Buffer (50 mM NaPi ...
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bioRxiv - Microbiology 2023Quote: ... 5’RACE was performed with SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc. San Jose, CA USA) according to the manufacturer’s directions.
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bioRxiv - Molecular Biology 2023Quote: ... including PCR using PrimeSTAR GXL (Takara), and DpnI treatment (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% Tet system-approved FBS (Takara #631368), 50 µg/mL hygromycin B ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: Cells were treated with 0-500 ng/mL doxycycline (Takara Bio) 48 h prior sample collection ...
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bioRxiv - Neuroscience 2023Quote: ... and the pEGFP-N1 backbone (Clontech)) by Golden Gate Assembly (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were lysed by sonication then purified using immobilized metal affinity chromatography (IMAC) resin charged with cobalt (TALON resin; Takara). Proteins were eluted with 200 mM Imidazole in buffer containing 20 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... using the Clontech In-Fusion HD Cloning System (Clontech #639645). The KPS2 and KPCt were genetically engineered to stably express the model antigen SIYx3-GFP by lentiviral transduction using the pLV-EF1a-SIYx3-GFP-IRES-Blast construct ...
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bioRxiv - Cancer Biology 2023Quote: ... The filtered virus was concentrated using the LentiX concentrator (Takara) at 1500 x g for 45 min ...
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bioRxiv - Bioengineering 2023Quote: ... qPCR was performed using TB Green® Premix Ex Taq™ II (Tli RNase H Plus) from TAKARA BIO USA INC (CA ...
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bioRxiv - Bioengineering 2023Quote: ... followed by cDNA synthesis using the cDNA Synthesis Kit (Cat# 6110A, Takara). All qRT-PCR primers were ordered from Eton Bioscience ...
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bioRxiv - Bioengineering 2023Quote: ... The process was performed on a 200 μL scale using 160 μL of His60 Ni-magnetic beads suspension (Takara Bio, Shiga, Japan). The beads were washed twice with 500 μL of sterilized water and 500 μL of Equilibration buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by RNA purification using NucleoSpin RNA kit (Takara Bio, Shiga, Japan) with on-column DNA digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... In-Fusion HD Cloning Kit (Clontech) was used for this reaction.
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... qTOWER3 G Real-Time System (Analytik Jena) Thermal Cycler Dice Real Time System III (Takara) or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...