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Citations for Takara Bio :
5551 - 5600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and 1 ng of embryonic total RNA from rabbits and cattle were reverse transcribed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio, Japan) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed using TaKaRa Ex Taq Hot Start Version (TaKaRa) with primers designed at 3’UTR of wallaby ZDBF2 according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli strains StellarTM (Takara bio, San Jose, CA, United States) were grown at 37°C in LB broth (Difco ...
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bioRxiv - Microbiology 2023Quote: ... sequence was replaced by that of virR (encoding amino-acids 1 to 164) or virRsol (encoding amino-acids 42 to 164) by In-Fusion HD cloning (Takara). PCR fragments were amplified using appropriate primer pairs (Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription and amplification were performed using One Step PrimeScript™ RT-PCR Kit (Takara, Japan) and reaction performed and visualized using Stratagene Mx3000P qPCR System real-time PCR system (Agilent ...
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bioRxiv - Microbiology 2023Quote: An LHCX MLV vector (Clontech) was engineered to express the N-terminal domains (RIGN ...
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bioRxiv - Immunology 2023Quote: ... T cells were transduced by centrifugation on Retronectin (Takara)-coated plates.
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bioRxiv - Immunology 2023Quote: ... Viral supernatants were concentrated 10-fold using RetroX concentrator (Takara).
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bioRxiv - Microbiology 2023Quote: ... HD In-Fusion kit (Takara 638909 In-Fusion® HD Cloning Plus) was used for cloning.
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bioRxiv - Microbiology 2023Quote: ... Mutation D382A in NP was generated with the In-Fusion cloning method (Takara 638909 In- Fusion® HD Cloning Plus). rLCMV/NP(D382A ...
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bioRxiv - Microbiology 2023Quote: ... these four fragments were fused together using the In-Fusion system (Takara Bio, Inc., Japan).
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bioRxiv - Immunology 2023Quote: Lenti-XTM 293T cells (Takara) were cultured at 0.5-1x106 cells/ml in Dulbecco’s modified Eagle’s Medium (DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 different reporters were tested in parallel: pE2F-TA-Luc (Clontech/Takara) to measure E2F transcriptional activity (repressed by Rb) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 different reporters were tested in parallel: pE2F-TA-Luc (Clontech/Takara) to measure E2F transcriptional activity (repressed by Rb) ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated for each sample using the SMART-Seq v4 kit (Takara). Samples were end-repaired ...
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bioRxiv - Molecular Biology 2023Quote: ... APEX2 and p110 were cloned into pLVX-IRES-puro (Takara, 632183) via a series of restriction enzyme digests and ligations ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was purified from N2 using Nucleospin RNA®(Takara Bio Inc., Kusatsu, Japan) as described in manufacture’s procedure ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA from a microgram of RNA was obtained using a PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan) following its instructions ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were transfected with a pEGFP-N2 plasmid from Clontech. Prior to incubation ...
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bioRxiv - Cell Biology 2023Quote: The following plasmids were commercially available or described previously: pEGFPC1 (Clontech), β-actin-GFP and β-actin-tdTomato [54] ...
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bioRxiv - Plant Biology 2023Quote: ... and histidine (-H) (TaKaRa 630319).
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bioRxiv - Bioengineering 2023Quote: ... All cloning was performed using the In-Fusion Cloning kit (Takara Bio) and ligated plasmids were transformed into chemically competent Stbl3 E ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... PrimeStar max DNA polymerase (Takara Bio, #R045A) and barcoded primers (Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lenti-X HEK293T cells were from Takara Bio ...
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bioRxiv - Molecular Biology 2023Quote: The Universal GenomeWalker 2.0 kit (Clontech Laboratories Inc. ...
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bioRxiv - Immunology 2023Quote: ... A cDNA library for TCR was prepared from RNA using a SMARTer Mouse TCR a/b Profiling Kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... about ∼5 μg of bacmid were transfected using 5 μL of TransIT®-Insect transfection reagent (Takara Bio Inc.). 5 days after initial transfection ...
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bioRxiv - Cell Biology 2023Quote: ... anti-GFP antibody (JL8) was from Clontech, and anti-Cdc37 antibody (E-4 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by gel purification (Takara Bio Cat. #740609.50). Samples were then pooled and sequenced on a lane of HiSeq 4000 SE50 at the UCSF Center for Advanced Technology (CAT).
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bioRxiv - Cell Biology 2023Quote: ... an EpiTaq hot start kit (TaKaRa Bio), a CloneJET PCR cloning kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: Lentivirus was made by transfecting LentiX-293T (Takara Bio) cells with Lenti-X Packaging Single Shot (Takara Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... and pRb-TA-Luc (Clontech/Takara) (promoter element directly suppressed by Rb) ...
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bioRxiv - Cancer Biology 2023Quote: ... and pRb-TA-Luc (Clontech/Takara) (promoter element directly suppressed by Rb) ...
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bioRxiv - Genomics 2023Quote: ... Libraries from immunoprecipitated DNA were generated using the SMARTer ThruPLEX DNA-seq Kit (Takara Bio), size-selected and quality assessed by Bioanalyzer DNA High Sensitivity chips (Agilent Technologies ...
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bioRxiv - Immunology 2023Quote: ... Mighty Mix (Takara Bio USA, San Jose, CA, USA), and transformed into chemically competent TOP10 E.coli cells (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Two-color FISH was conducted as previously described with minor adaptations.20,21 Undifferentiated ChiPSC22 were seeded on DEF-CS COAT-1-coated (Cellartis, Takara BioSciences) coverslips and differentiated ChiPSC22 on poly-L-lysine-coated coverslips ...
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bioRxiv - Cell Biology 2023Quote: ... and libraries were generated by LIANTI40 or PicoPLEX Gold (Takara Bio). Libraries were sequenced by NOVAseq (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: Human DHRS7 was expressed as a GFP fusion using the pEGFP-N2 vector (Clontech) generated previously [5] ...
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bioRxiv - Microbiology 2023Quote: As a basis for minimal lentiviral vectors the pLVX-IRES-Neo vector (Clontech) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were concentrated using the LentiX Concentrator (Clontech). Organoids were trypsinized into small cell clusters and incubated with the concentrated virus particles supplemented with 4 μg/mL Polybrene (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus reagent (TaKaRa), and cDNA was synthesized from 0.5 µg of each RNA preparation using the ReverTra Ace qPCR RT kit (TOYOBO ...
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bioRxiv - Cancer Biology 2023Quote: ... was cultivated in the feeder-free DEF-CS system (Cellartis/Takara Bio Europe) under standard conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... Constructs were ligated (In-Fusion HD, Takara) into pLenti vector behind an EFS promoter using BamHI and NsiI sites ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 ng/mL doxycycline (Takara Bio) was added to the media and cells were incubated for 48 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 0-500 ng/mL doxycycline (Takara Bio) and imaged in an Incucyte Live-Cell Analysis system (Sartorius ...
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bioRxiv - Cancer Biology 2023Quote: ... concentrated 10x using LentiX concentrator reagent (Takara Bio 631231) and preserved in 0.4 ml aliquots at −80C.
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bioRxiv - Cancer Biology 2023Quote: ... using CalPhos™ Mammalian Transfection Kit (Clontech, Inc., Mountain View, CA, USA), according to the manufacturer’s protocol as described (Wenta et al ...
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bioRxiv - Cancer Biology 2023Quote: ... Human iPSC line ChiPSC22 (Cellartis/Takara Bio Europe AB) was cultivated in the feeder-free DEF-CS system (Cellartis/Takara Bio Europe ...
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bioRxiv - Cell Biology 2023Quote: Lentivirus was generated in a 10cm dish format by transfecting HEK293T Lenti-X cells (Takara) at roughly 80% confluency with 4.5μg of an equimolar mixture of three plasmids encoding the envelope and viral packaging components from the laboratory of Didier Trono (pMD2.G ...
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA was synthesized using PrimeScript RT reagent Kit (Perfect Real Time, TAKARA BIO Inc. Shiga, Japan). The mouse aorta was homogenized by TissueLyser II (Qiagen ...