Labshake search
Citations for Takara Bio :
5501 - 5550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... 0.5U/μL RNAse inhibitor (Takara, 2313A), 0.5% biocytin and 0.02 Alexa 488 ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV stock concentration was determined using the Takara AAV Titration kit (Takara #6233) and an Applied Biosystems 7500 Fast Real-Time PCR System.
-
bioRxiv - Neuroscience 2023Quote: ... 2 μg/ml doxycycline (Clontech, cat. no. 631311), 1X N2 supplement (Life Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... 2 μg/ml doxycycline (Clontech, cat. no. 631311), 1X N2 supplement (Life Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... 7 million Lenti-X HEK-293T cells (Takara Bio, cat. no. 632180) were seeded in 9 mL DMEM (Gibco ...
-
bioRxiv - Neuroscience 2023Quote: ... supplemented with 6 ml Lenti-X Concentrator (Takara Bio; Cat. No. 631231), mixed thoroughly ...
-
bioRxiv - Neuroscience 2023Quote: ... Pelleted nuclei were then washed in 4 mL of Nuclei Suspension Buffer containing 0.2% RNase inhibitor (Clontech/Takara, Cat. 2313B) and centrifuged at 500xg for five minutes at 4°C ...
-
bioRxiv - Pathology 2023Quote: ... and reverse transcribed using PrimeScript RT Master Mix for RT-PCR (Takara Bio, Japan). Real-time PCR was performed using SYBR Select Master Mix (Applied Biosystems ...
-
bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized with the PrimeScript 1st Strand kit with an additional primer mix containing random DTs (#RR047A and #6110A, Takara). cDNAs were amplified using specific Taqman probes ...
-
bioRxiv - Pathology 2023Quote: RNA from tissues was isolated using TRIzol reagent (Takara Bio, Japan) and reverse transcribed using PrimeScript RT Master Mix for RT-PCR (Takara Bio ...
-
bioRxiv - Pathology 2023Quote: Total RNA was extracted from skin biopsies and cell lysates using RNAiso Plus Reagent (Takara, Cat No: 9109) according to the manufacturer’s protocol ...
-
bioRxiv - Pathology 2023Quote: ... cDNA was prepared using PrimeScript cDNA synthesis kit (Takara, Cat No: 2680A) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2023Quote: ... and RNA was isolated using TRIzol (Takara, Japan). The quality and quantity of RNA were analyzed using NanoDrop2000 (Thermo Scientific ...
-
bioRxiv - Pathology 2023Quote: ... The cDNA was prepared from the extracted RNAs with oligo (dT) and random primers using the PrimeScript RT reagent kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... and ARR1 (2070 bp) was generated using cDNA of Col-0 as template and ligated into T-vector pMD20 (Takara Bio, Japan). All the primers used for cloning were listed in Table S1 ...
-
bioRxiv - Plant Biology 2023Quote: ... and MS11-MN177 (SH3P2(BAR)) and cloned between the EcoRI and XhoI and EcoRI and BamHI restriction sites of pGADT7 (Clontech) respectively ...
-
bioRxiv - Plant Biology 2023Quote: ... Quantitative α-galactosidase assay was also performed according to manufactural instruction (Clontech). Briefly ...
-
bioRxiv - Plant Biology 2023Quote: ... and then cloned into pENTR/D/SD entry vector using the In-Fusion cloning kit (Takara). Inserts of all plasmids were sequenced by Macrogen ...
-
bioRxiv - Plant Biology 2023Quote: ... 1 μg total RNA was reverse transcribed into cDNA with the SMARTer RACE 5’ Kit (Clontech). PCR was performed using primer GSP-HO1 and the Universal Primer Mix (UPM ...
-
bioRxiv - Plant Biology 2023Quote: Y2H assays were performed using the MatchmakerTM Two-Hybrid System (Clontech). Bait (pGBKT7 ...
-
bioRxiv - Systems Biology 2023Quote: ... before library preparation using the SMARTer Human TCR α/β Profiling Kit (Takara Bio). In brief ...
-
bioRxiv - Plant Biology 2023Quote: ... pGADT7 plasmids encoding the effector domain of different ZiF were co-transformed into chemically competent Y2HGold cells (Takara Bio, USA) with a pGBKT7 plasmid encoding OsExo70F3 in using a Frozen-EZ Yeast Transformation Kit (Zymo research).
-
bioRxiv - Plant Biology 2023Quote: ... and terminator was assembled into the backbone PCB1532 via In-Fusion cloning (In-Fusion cloning kit, Clontech Laboratories).
-
bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was removed using DNase I (TaKara). RNA-seq transcriptome libraries were prepared using the TruSeq RNA sample preparation kit from Illumina ...
-
bioRxiv - Plant Biology 2023Quote: ... and on a SD-Leu-Trp-Ade-His plate containing X-α-gal and supplemented with 0.2 µg/ml Aureobasidin A (Takara Bio, USA). Plates were imaged after incubation for 60–72 hr at 30 °C ...
-
bioRxiv - Plant Biology 2023Quote: ... with SYBR premix ExTaq (Tli RNaseH Plus) Rox Plus (Takara Bio Inc). Enrichment in each sample was calculated relative to the input and expressed in percentage.
-
bioRxiv - Neuroscience 2023Quote: Cultured neurons were transfected using the CalPhos Mammalian Transfection Kit (Takara Cat# 631312). Briefly ...
-
bioRxiv - Plant Biology 2023Quote: ... Fragments amplified with primers for CAPS markers were digested with appropriate restriction enzymes supplied by Takara Bio (Kusatsu ...
-
bioRxiv - Plant Biology 2023Quote: ... the genomic sequence from the MpRKD promoter region (3827 bp upstream from start codon) to the 3’UTR was amplified by PCR with PrimeSTAR HS DNA polymerase (TaKaRa Bio, Kusatsu, Japan) and the primers IF-Hind-pRKD-F and IF-Sal-gRKD-R ...
-
bioRxiv - Neuroscience 2023Quote: ... cDNAs were prepared with Clontech Smart-seq v4 ultra-low kit (Cat# 634888, Takara Bio, Shiga, Japan). PolyA tail enrichment was done using NEBNext® Poly(A ...
-
bioRxiv - Plant Biology 2023Quote: ... The DNA segments coding MIR319A and MIR319B loci (with genomic DNA as the template) were amplified using PrimeSTAR HS DNA polymerase (TaKaRa Bio, Kusatsu, Japan) with miR319aF-miR319R or miR319bF-miR319R primer pairs and were sequenced according to Sanger method ...
-
bioRxiv - Neuroscience 2023Quote: ... Constructs containing point-mutated ARE-like sequences were prepared using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Because an ARE half-site can function alone to confer androgen inducibility (Pihlajamaa et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... or control mCherry were subcloned using the InFusion HD cloning kit (Takara Bio USA). Furthermore ...
-
bioRxiv - Plant Biology 2023Quote: ... paired-ended libraries were constructed using SMART ChIPseq kit (TAKARA) and sequenced using HiseqX ...
-
bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed with GeneAce SYBR qPCR Mix α No ROX (Nippon Gene, Tokyo, Japan) in Thermal Cycler Dice Real Time System II (Takara). Each Actin1 genes (PtActin1 and TpActin1 ...
-
bioRxiv - Plant Biology 2023Quote: ... Yeast-two-hybrid library screens were carried out according to Matchmaker® Gold Yeast Two-Hybrid System User Manual (Takara Clontech Bio Europe, PT4084-1) using a cDNA library of M ...
-
bioRxiv - Plant Biology 2023Quote: ... Yeast-two-hybrid library screens were carried out according to Matchmaker® Gold Yeast Two-Hybrid System User Manual (Takara Clontech Bio Europe, PT4084-1) using a cDNA library of M ...
-
bioRxiv - Plant Biology 2023Quote: ... HRP substrates (Takara) and an iBrightTM CL750 Imaging System were used for immunodetection.
-
bioRxiv - Neuroscience 2023Quote: ... ds-cDNA was generated using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
-
bioRxiv - Physiology 2023Quote: ... PCR products were cleaned with NucleoMag beads (Takara Bio, Catalog #744503) and then visualized using gel electrophoresis ...
-
bioRxiv - Plant Biology 2023Quote: ... MtU2AF35b and RiSP7 or MtSR45 truncation constructs were amplified with restriction sites and subsequently cloned into the pGBKT7 or pGADT7-REC vectors (Takara Clontech Bio Europe ...
-
bioRxiv - Plant Biology 2023Quote: ... the correct full-length sequences were determined for the laboratory strains using a SMARTer RACE 5’/3’ kit (Takara, Shiga, Japan). These sequences were aligned by ClustalW ...
-
bioRxiv - Molecular Biology 2023Quote: ... 500 ng of the total RNA was used for cDNA synthesis using the Prime Script 1st Strand cDNA Synthesis Kit (TaKaRa, 6110A). Random 6-mer primers (0.3 µL ...
-
bioRxiv - Molecular Biology 2023Quote: The commercially available pEGFP-C2 (Clontech) plasmid was used as a sense EGFP construct ...
-
bioRxiv - Microbiology 2023Quote: ... SEAP activity was quantified using the Great EscAPe SEAP assay (Clontech). Fluorescence was read on a Filtermax F5 plate reader (Molecular Devices).
-
bioRxiv - Microbiology 2023Quote: ... subtilis 168 and the chloramphenicol resistance gene was amplified from plasmid pDG1662 according to the manufacturer’s instruction (Takara Primestar max DNA polymerase). These three fragments were combined by overlapping Polymerase Chain Reaction (PCR ...
-
bioRxiv - Neuroscience 2023Quote: ... Arr3 and Arr3-derived constructs were detected with rabbit anti-Arr3 (Ahmed et al., 2007) or mouse anti-GFP (Clontech JL-8) antibody ...
-
bioRxiv - Microbiology 2023Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
-
bioRxiv - Molecular Biology 2023Quote: HEK293T/17 cells were co-transfected with 0.8 pmol SINEUP-GFP or miniSINEUP-GFP FRAM (0.8 pmol) and 0.2 pmol sense pEGFP-C2 plasmids (Clontech) in each well of a 6-well culture plate ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 µL of RNaseH (TaKaRa, 2150A), and 1 µL of RNase cocktail (Invitrogen ...