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Citations for Takara Bio :
5451 - 5500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... DNA amplification of phage or bacterial genetic sequences were performed by PCR using the CloneAmp HiFi PCR premix® (Takara©). Primers used in this study are listed in Table S1 ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast cells were cultivated on minimal synthetic defined (SD) media (Clontech), lacking Leu (selection for pACT2-GW ...
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bioRxiv - Plant Biology 2023Quote: ... The quantitative real-time polymerase chain reaction was performed using the ABI 7300 sequencer and SYBR Premix Ex Taq-TM (RR420, Takara, Kyoto, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... were purchased from Clontech (Beijing). The pGD-3GmCherry was a vector modified by pGD (Sun et al. ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand cDNA was synthesized with a PrimeScript™ RT reagent kit with gDNA Eraser (Takara). For gene expression pattern analysis ...
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bioRxiv - Plant Biology 2023Quote: ... Purified PCR products were cloned into pYAO::hSpCas9 plasmid via In-Fusion (Takara, 639650).
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bioRxiv - Plant Biology 2023Quote: ... 4 μg of total RNA was used for cDNA synthesis with PrimeScript 1st strand cDNA Synthesis Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and the resulting PCR product was cloned into the epiGreenB5 (3xHA) and epiGreenB (eGFP) vectors between the ClaI and BamHI restriction sites with an In-Fusion HD Cloning Kit (Clontech, CA, USA) (Nekrasov et al. ...
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bioRxiv - Plant Biology 2023Quote: ... 0.05% T-20 and protease inhibitor cocktail (Takara)] ...
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bioRxiv - Plant Biology 2023Quote: ... and ARR1 (2070 bp) was generated using cDNA of Col-0 as template and ligated into T-vector pMD20 (Takara Bio, Japan). All the primers used for cloning were listed in Table S1 ...
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bioRxiv - Plant Biology 2023Quote: ... and MS11-MN177 (SH3P2(BAR)) and cloned between the EcoRI and XhoI and EcoRI and BamHI restriction sites of pGADT7 (Clontech) respectively ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative α-galactosidase assay was also performed according to manufactural instruction (Clontech). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... and then cloned into pENTR/D/SD entry vector using the In-Fusion cloning kit (Takara). Inserts of all plasmids were sequenced by Macrogen ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg total RNA was reverse transcribed into cDNA with the SMARTer RACE 5’ Kit (Clontech). PCR was performed using primer GSP-HO1 and the Universal Primer Mix (UPM ...
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bioRxiv - Plant Biology 2023Quote: Y2H assays were performed using the MatchmakerTM Two-Hybrid System (Clontech). Bait (pGBKT7 ...
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bioRxiv - Systems Biology 2023Quote: ... before library preparation using the SMARTer Human TCR α/β Profiling Kit (Takara Bio). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... First strand cDNA synthesis was performed on 200ng RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Reverse transcription (RT)-PCR was performed using the Emerald Amp MAX PCR master mix (Takara Bio, Shiga, Japan). Quantitative RT-PCR was performed using THUNDERBIRD SYBR qPCR MIX (TOYOBO Life Science ...
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bioRxiv - Systems Biology 2023Quote: ... using the TB Green Advantage qPCR premix (Takara Bio, Kutatsu, Japan) and the oligonucleotides specified in Table 1 ...
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bioRxiv - Plant Biology 2023Quote: ... 500 ng total RNA was reverse transcribed into cDNA with the PrimeScript RT Master Mix (TaKaRa). qPCR was performed using corresponding primers listed in SI Appendix ...
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bioRxiv - Plant Biology 2023Quote: ... PCR was performed using primer GSP-HO1 and the Universal Primer Mix (UPM, Clontech) with KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA libraries for each sample were constructed from 100 ng of RNA using the PrimeScript RT master mix (Takara Bio, Kutatsu, Japan) under manufacturer specifications ...
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bioRxiv - Physiology 2023Quote: Approximately 70-80% confluent HEK293T cells (AAVpro® 293T Cell Line; Takara # 632273 AAVpro® 293T Cell Line; Takara # 632273) in DMEM (SH30022.01 ...
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bioRxiv - Physiology 2023Quote: Approximately 70-80% confluent HEK293T cells (AAVpro® 293T Cell Line; Takara # 632273 AAVpro® 293T Cell Line ...
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bioRxiv - Physiology 2023Quote: ... Titering was performed on Ad293 cells using Adeno-X Rapid Titer kit (Clontech) according to the instructions of the manufacturer 28.
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bioRxiv - Plant Biology 2023Quote: ... and converted to cDNA using PrimeScript™ IV 1st strand cDNA Synthesis Mix (DSS Takara Bio India Pvt. Ltd., New Delhi, India). QPCR was carried out using Brilliant II SYBR master mix (Agilent Technologies India Pvt Ltd ...
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bioRxiv - Plant Biology 2023Quote: ... was inserted using the in-fusion cloning technique (Clontech).
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bioRxiv - Plant Biology 2023Quote: ... which was then reverse-transcribed into first-strand cDNA using the Prime-Script-TM RT Reagent Kit (RR036, Takara, Kyoto, Japan). The quantitative real-time polymerase chain reaction was performed using the ABI 7300 sequencer and SYBR Premix Ex Taq-TM (RR420 ...
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bioRxiv - Cell Biology 2023Quote: ... which was cloned into the pEGFP-C3 vector (Clontech, Mountain View, CA, USA) using the In-Fusion HD cloning kit (Takara Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... pLKO.1-shFEN1 or pLKO.1 control vector was simultaneously transfected into LentiX293T (Cat# 632180 Clontech, Japan) with packaging plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... or 100 ng of genomic DNA was amplified with Advantage GC 2 polymerase (Takara Bio 639114) using 1 M GC Melt and primers spanning the region from upstream of the splice donors to the 3’ end of the repeat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries construction (SMART-Seq v4 Ultralow input, Takara) with barcoded adaptors as well as RNA sequencing on NextSeq500-Illumina (single-end 1×5 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting cDNAs were PCR-amplified using LA Taq DNA polymerase (TaKaRa), a common PCR primer used for all library amplifications ...
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bioRxiv - Neuroscience 2023Quote: ... Cell debris was filtered from the collected medium and Lenti-X Concentrator (Clontech) was then used to precipitate the lentivirus in the medium ...
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bioRxiv - Neuroscience 2023Quote: ... We used the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, 634894) to reverse transcribe poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... IHC was conducted using rabbit primary antibody dsRed (1:500, #632496, Takara) to label TdT+ cells ...
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Multi-omics analysis reveals signatures of selection and loci associated with complex traits in pigsbioRxiv - Genomics 2023Quote: ... and then reverse-transcribed to cDNA using the PrimeScript™ RT Master Mix (Perfect Real Time) (Takara, Beijing, China) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2023Quote: ... double-stranded DNA library libraries from the size fractionated samples were constructed using the ThruPLEX DNA-seq Kit (Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 40U/mL of RNase inhibitor (Takara: 2313B)] ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney 293T (HEK293T/HEK293LE; Clontech 632180) cells were cultured in DMEM with 10% fetal bovine serum (Fisher #10082-147 ...
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bioRxiv - Neuroscience 2023Quote: ... Clontech Advantage PCR buffer (1x) and mix (1x) (TaKaRa), linker-ligated DNA (0.6 v/v ratio) ...
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bioRxiv - Neuroscience 2023Quote: ... mCherry-C1 plasmid (Clontech, Mountain View, CA, USA) at 1 ng/μl ...
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bioRxiv - Neuroscience 2023Quote: ... eGFP plasmid (Clontech) at 5 ng/μL and pOCTmCherry at 1 μg/mL (Tradewell et al ...
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bioRxiv - Microbiology 2023Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313A)] and incubated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 90 μl of RNase free water was added and then 2.5 μl of diluted sample was used for real-time RT-PCR according to the manufacturer’s protocol with One step TB green PrimeScript PLUS RT-PCR Kit (Takara, Cat# RR096A) and primers for Nucleocapsid (N ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Microbiology 2023Quote: ... This clone was digested with NsiI and BglII to remove the CMV promoter sequence and was combined with the pSV010(-) SV40 promoter fragment using the Infusion® HD Cloning kit (Clontech) such that the pSV010(- ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP 61,62 (400ng) using TransIT-LT1 (Takara, Cat# MIR2306). On day 3 (24 hours post transfection) ...
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bioRxiv - Microbiology 2023Quote: ... The crude lysates were centrifuged at 35’000 g for 30 min and the cleared lysates incubated with 200 μL Ni-NTA agarose beads (Takara Bio, Shiga, Japan) for 1h at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The viral particles in the supernatant were then concentrated using LentiX Concentrator (Takara Bio), in accordance with the manufacturer’s instructions and resuspended at 10X final concentration in PBS.