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Citations for Takara Bio :
5351 - 5400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The LexA fusion constructs (bait) and the activation domain fusion constructs (prey) were co-transformed into yeast strain EGY48[p8op-lacZ] (CLONTECH). Clones containing both constructs were selected on medium lacking His ...
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bioRxiv - Molecular Biology 2023Quote: ... pKJE7 (Takara) was used to supply DnaKJ to the dnaK-dnaJ deleted cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... All constructs were cloned via In-Fusion HD Cloning (Takara Bio).
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bioRxiv - Synthetic Biology 2023Quote: ... protein-coding sequences for Tluc were replaced with corresponding fragments through In-Fusion cloning (Takara; 638948) or restriction cloning strategies ...
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bioRxiv - Synthetic Biology 2023Quote: ... and used directly or 10x concentrated using LentiX Concentrator (Takara Bio) following manufacturers instructions prior to adding to cell lines.
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bioRxiv - Plant Biology 2023Quote: ... transferred membranes were hybridized overnight at 4 °C with the following primary antibodies: anti-GFP (Takara Bio Cat# 632381, RRID:AB_2313808) (1/5000) ...
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293 cells (Takara 632180), C3H/10T1/2 Clone 8 (ATCC# CCL-226) ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-DsRed (1:2,000, Takara Bio Inc.), rabbit or guinea pig anti-Discs large (1:30,000 and 1:1.000 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1%Triton X) and then resuspended in lambda DNA solution (diluted to approximately 20 ng/µL in TE solution, 3010, Takara). For the Sera-Mag™ Select (29343052 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µl/well of beads were washed twice with TE solution and then resuspended in lambda DNA solution (diluted to approximately 20 ng/µL in TE solution, 3010, Takara). Then ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... The paraffin sections were de-waxed and stained with primary antibody OCN (#M173, Takara Bio, Japan, 1:100), and counterstained with Harris Hematoxylin.
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bioRxiv - Molecular Biology 2023Quote: ... along with the hyperactive PiggyBac Transposase (hyPBase) utilizing Xfect transfection reagent (Takara-Bio, San Jose, California). Transposase and Transposon DNA were added at 1:5 ratio in Xfect transfection reagent per manufacturer instructions to form the nanoparticle transfection complex ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR reactions were set up with FastStart DNA Master SYBR Green I master mix (Takara, Japan). For each analysis ...
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bioRxiv - Plant Biology 2023Quote: ... The restriction enzyme Dpn I (Takara-Bio, Kusatsu, Japan) was added to the PCR product and the solution was incubated at 37°C for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... anti-DsRed (Takara Bio Cat# 632496, RRID:AB_10013483) (1/5000) ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using the SYBR premix ExTaq II kit (TAKARA, RR820A) on the BioRad CFX96 system ...
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bioRxiv - Plant Biology 2023Quote: Total RNAs from inflorescences or seedlings were treated with DNase I followed by reverse transcription using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TAKARA, 6210A) with oligo-d(T ...
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bioRxiv - Plant Biology 2023Quote: ... the plasmid was constructed using the In-Fusion HD Cloning Kit (Takara); 1–2 kb sequences of the 5’ and 3’ ends of the genes of interest flanked the fragment that consisted of an in-frame linker ...
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bioRxiv - Neuroscience 2023Quote: ... with a HA tag and deletion mutants of Lag3 D1 domain with a myc tag were constructed by PCR with In-Fusion HD Cloning Plus systems (Takara; Bio Inc., Otsu, Japan) using the CloneAmp™ HiFi PCR Premix (a high-fidelity PCR polymerase included with all In-Fusion HD Cloning Plus Systems) ...
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bioRxiv - Neuroscience 2023Quote: ... In-Fusion HD enzyme premix was added to the linearized PCR product and transformed into the Stellar™ Competent Cells (Takara; Bio Inc., Otsu, Japan). Integrity of plasmid sequences was verified through sequencing.
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bioRxiv - Plant Biology 2023Quote: For Yeast-two-Hybrid (Y2H) experiments coding DNA sequences were cloned into pDONR207 and recombined into pGDAT7® or pGBKT7® vectors (Clontech). Plasmids were transformed into the AH109 yeast strain by the lithium acetate (LiAc ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into the empty pCambia1305-3xFLAG or pCambia1305-3xHA vectors (gifted by Dr Yuli Ding) which were pre-digested with DraIII (Takara). All constructs were verified by sequencing and introduced into Agrobacterium tumefaciens GV3101 strain for transformation in N.benthamiana leaves.
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bioRxiv - Plant Biology 2023Quote: ... Positive yeast colonies were selected and confirmed by using different synthetic drop-out (SD) (Takara) media prepared according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... polystyrene plates were coated overnight with 20 µg/mL retronectin (Takara Bio.) in PBS at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized with 2 μg RNA using PrimeScript™ 1st strand cDNA synthesis kit (TaKaRa, Kusatsu, Shiga, Japan) in a final reaction volume of 20 μl following the manufacturer’s protocol.
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bioRxiv - Plant Biology 2023Quote: ... the cDNA was amplified by PCR using the Ex Taq DNA Polymerase (TaKaRa, Tokyo, Japan) with primers located within exon-3 and exon-4 ...
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bioRxiv - Molecular Biology 2023Quote: ... and treated with recombinant DNase I (Takara). The treated RNA was subsequently purified using an RNeasy Mini kit (Qiagen) ...
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bioRxiv - Plant Biology 2023Quote: ... each egg cell sample was transferred to a lysis buffer supplied in the SMART-Seq HT kit (Takara Bio, Shiga, Japan). The lysates were immediately used for cDNA synthesis or frozen in liquid nitrogen and stored at -80 °C ...
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bioRxiv - Plant Biology 2023Quote: The truncated version of the RPF2nad6 was generated by PCR amplification using Takara PrimeStar polymerase (TaKaRa) and a primer pair designed to remove the last 180 nucleotides of the RPF2nad6 coding sequence representing the RfCTD sequence (Supplementary table 6) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized and amplified from cell lysates using a SMART-Seq HT Kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplified and subcloned into a pCG-strep expression construct using the In-Fusion® HD Cloning Kit (Takara) according to the manufacture’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... or S387T were generated using the In-Fusion® HD Cloning Kit (Takara). SARS-CoV-2 Spike BA.2 spike with mutations of Δ69-70 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit-anti mCherry (Takara Bio Clontech; 632496), rabbit anti-CART (55–102 ...
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bioRxiv - Microbiology 2023Quote: ... terminator sequences and miniTn7 elements were amplified using PrimerStar Max DNA Polymerase from Takara (templates indicated in supplemental table S3) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 1 μg/ml Proteinase K (Takara Bio, Shiga, Japan) treatment ...
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bioRxiv - Genomics 2023Quote: Specimens were run in technical duplicates for tprK deep sequencing using 10.5µL DNA added to 12.5µL 2X CloneAmp Pre-mix (Takara), 1µL 10µM barcoded tprK-F and 1µL 10µM barcoded tprK-R (Table S2 in Supplemental Data) ...
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bioRxiv - Genomics 2023Quote: ... 12.5µL 2X CloneAmp Pre-Mix (Takara) and 1µL each 10µM of tpdel-F (5’-TGCTCTCGCATGGCTATGTT-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary hippocampal cultures were transfected using CalPhos mammalian transfection kit (Clontech, 631312) as previously (Jiang & Chen ...
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bioRxiv - Pathology 2023Quote: ... Then reverse transcription reaction was conducted by means of TaKaRa reverse transcription reagents (TaKaRa, Dalian, China). The quantitative real-time polymerase chain reaction was performed using an SYBR Premix ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA of cell was extracted by Trizol reagent (Takara, Japan), and then transcribed into cDNA by Hifair® II 1st Strand cDNA Synthesis Kit (Yeasen ...
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bioRxiv - Cell Biology 2023Quote: ... PCRs were carried out using HiFi polymerase (Takara Bio UK Ltd) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Each cDNA was synthesized using a PrimeScript RT reagent kit (Takara Bio, Kusatsu-Shiga, Japan). Quantitative PCR reactions were performed using SYBR Premix Ex Taq II with a thermal cycler (TP800 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR reactions were performed using SYBR Premix Ex Taq II with a thermal cycler (TP800, Takara Bio).
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bioRxiv - Molecular Biology 2023Quote: ... and first-strand cDNA was synthesized using PrimeScript RT Master Mix (Perfect Real Time) (RR036Q; TaKaRa, Ohtsu, Japan) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Lentivirus (LVs) were collected by ultra-speed centrifugation and purified with the Lenti-X concentrator (Takara) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Real-time PCR was performed using THUNDERBIRD Next SYRB qPCR Mix (TOYOBO) on a real-time PCR system (Thermal Cycler Dice® Real Time System, Takara).
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bioRxiv - Microbiology 2023Quote: ... The specific amplicons were cloned into pMD18T vectors (TaKaRa, Dalian, China), and 10 clones of each amplicon were randomly picked for sequencing by the Sanger method on an ABI 3730xl DNA sequencer (ComateBio ...
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bioRxiv - Microbiology 2023Quote: ... The V1-V2 variable region of stool DNA was amplified by universal primer set 27F-mod (5’-AGRGTTTGATYMTGGCTCAG-3’) and 338R (5’-TGCTGCCTCCCGTAGGAGT-3’) using Gflex DNA polymerase (Takara) 37 ...
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bioRxiv - Immunology 2023Quote: ... 0.5 μl RNAse inhibitor (Clontech), 4 μl Superscript II First-Strand Buffer (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using SMARTer RACE 5’/3’ Kit (Takara Bio, Shiga, Japan). ssRNA was converted into cDNA using SMARTer Universal Low Input RNA Kit according to the manufacturer’s protocol (Takara Bio) ...