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Citations for Takara Bio :
5301 - 5350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... LipofectamineTM 2000 kit and reverse transcription kit were purchased from TaKaRa Company ...
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bioRxiv - Cancer Biology 2023Quote: ... and developed using Western BLoT Chemiluminescent HRP Substrate (Takara). The chemiluminescent images were observed using FUSION FX (Vilber ...
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bioRxiv - Cancer Biology 2023Quote: ... detected with Western BLoT Rapid Detect v2.0 (Takara, Shiga, Japan), and developed using Western BLoT Chemiluminescent HRP Substrate (Takara) ...
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bioRxiv - Biochemistry 2023Quote: ... or the EmeraldAmp GT PCR Master Mix (Takara Bio). Primers were obtained from Merck or Eurofins Genomics ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μl of cell extract was used in a buffer containing final concentrations of 52 mM HEPES pH 7.4 (Takara), 35 mM KGlu (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were collected at 36 and 72 h and then purified with 10% Lenti-X Concentrator® (Clontech, Mountain View, CA, USA). The virus copy number was determined by Q-PCR ...
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bioRxiv - Cancer Biology 2023Quote: Using Ex Taq DNA Polymerase (Takara), sgRNA inserts from each initial and final pool were PCR-amplified from 144 μg of gDNA to achieve ∼400-fold coverage of the library ...
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bioRxiv - Neuroscience 2023Quote: ... a Living Colors® EGFP mouse monoclonal antibody (632569, Clontech), or a mouse monoclonal anti-actin antibody (clone AC-40 ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Wild-type (WT) TIPRL1 cDNA was cloned into 3xFLAG pLenti6.4 using In-Fusion® HD Cloning Kit (Takara, #639650). Phosphomimic and non-phospho mutants (S265D and S265A ...
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bioRxiv - Plant Biology 2023Quote: ... and then 1 mL of RNAiso Plus (Takara Bio., Japan) was added and thoroughly mixed ...
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bioRxiv - Plant Biology 2023Quote: ... TB GreenTM Premix Ex TaqTM (Takara) was used as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: shRNA constructs were engineered on a pSIREN retroviral vector (Clontech). To deplete the endogenous expression of BIN1 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (634898, Takara Bio.). The cDNA quality was examined on Agilent TapeStation system (RRID:SCR_019547 ...
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bioRxiv - Plant Biology 2023Quote: All yeast work was performed according to the yeast protocols handbook (Clontech, Mountainview, CA). For Y2H screening ...
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bioRxiv - Plant Biology 2023Quote: The full-length MpACL5 amplified from Tak-1 cDNA by PCR using TaKaRA EX Taq (Takara, Shiga, Japan) with the primers ...
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bioRxiv - Plant Biology 2023Quote: ... and gametandiopore of one-month-old Tak-1 and Tak-2 by NucleoSpin RNA Plant (Takara) or Monarch Total RNA Miniprep Kit (New England BioLabs ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified from Escherichia coli BL21 (DE3) with TALON Metal Affinity Resin (Takara), Pierce Glutathione Agarose (Thermo scientific) ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid assays were performed using strain AH109 (Takara). Vectors pGADT7 and pGBKT7 were sequentially transformed into this strain ...
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bioRxiv - Plant Biology 2023Quote: Y1H assay was conducted according to protocol (Clontech, cat. No. 630491). Briefly ...
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bioRxiv - Plant Biology 2023Quote: Y2H assay was performed according to protocol (Clontech, cat. No. 630489). Since BcICE1 self-activates ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with primary goat anti-dsRED (1:1000, Takara Bio, Cat# 632496, RRID: AB_10013483), goat anti-ChAT (1:400 ...
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bioRxiv - Systems Biology 2023Quote: ... We modified the Gal4 Matchmaker Gold Yeast Two-hybrid system (clonetech) pGBK_T7 (Clontech) expression vector to harbor the pGADT7_AD vector’s multiple cloning site (MCS ...
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bioRxiv - Systems Biology 2023Quote: ... using infusion recombinase cloning (Clontech). We modified the Gal4 Matchmaker Gold Yeast Two-hybrid system (clonetech ...
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bioRxiv - Microbiology 2023Quote: ... in equimolar concentrations into Lenti-X 293T cells (Takara Bio) using Lipofectamine 3000 reagent (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA encoding HsPINK1 was inserted into the BamHI site of pcDNA5/FRT/TO CMVd3 vector (pcDNA5d3, see (17)) using the In-Fusion HD Cloning Kit (Takara). For stable expression ...
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bioRxiv - Systems Biology 2023Quote: HEK293XT (Takara Bio Lenti-X 293T, #632180) cells were cultured in DMEM containing L-glutamine and sodium pyruvate supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Biochemistry 2023Quote: ... The annealed substrate was purified using CHROMA SPIN™+TE-200 Columns (TaKaRa) to remove unannealed oligonucleotides.
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bioRxiv - Cell Biology 2023Quote: ... Inc. PrimeSTAR HS polymerase (cat. R40A) was purchased from Takara. Polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: ... and primers containing approximately 500 base pairs sequence homologous to the target locus and then cloned into the EcoRV site of pBluescript SK+ using an In-Fusion HD Cloning Kit (Takara Bio). TP53-missense mutations and protospacer adjacent motif (PAM ...
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bioRxiv - Cell Biology 2023Quote: ... lenti-X 293T Cells (Takara Bio) were co-transfected with Lentiviral High Titer Packaging Mix (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... using an In-Fusion HD Cloning Kit (Takara Bio). To produce lentiviral stocks ...
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bioRxiv - Biochemistry 2023Quote: ... 1,5 µg of plasmid DNA and 1,5 µg pOG44 (encoding the Flp recombinase) were mixed and transfected using CalPhos Mammalian Transfection Kit (Clontech®/Takara, #631312). The transfection medium was replaced by complete DMEM after 24 h ...
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bioRxiv - Biochemistry 2023Quote: ... 1,5 µg of plasmid DNA and 1,5 µg pOG44 (encoding the Flp recombinase) were mixed and transfected using CalPhos Mammalian Transfection Kit (Clontech®/Takara, #631312). The transfection medium was replaced by complete DMEM after 24 h ...
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bioRxiv - Microbiology 2023Quote: ... RNA was prepared for sequencing using the SMARTER Stranded RNA sequencing kit (Takara Bio) according to the manufacturer’s instructions with the following parameters ...
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bioRxiv - Cell Biology 2023Quote: ... using PrimerSTAR® Max DNA Polymerase (Takara Bio Inc., Shiga, Japan) to obtain the fragments of SC and CS chimaera ...
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bioRxiv - Biochemistry 2023Quote: The reporter plasmids were linearized with XbaI (Takara). Subsequently ...
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bioRxiv - Biochemistry 2023Quote: ... coli Stellar Competent Cells (Takara) and the DNA sequences were verified by Sanger sequencing.
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bioRxiv - Biochemistry 2023Quote: ... Constructs used to test protein-protein interactions were co-transformed into the yeast Saccharomyces cerevisiae strain AH109 using the Matchmaker Gold yeast two-hybrid system (Clontech). Empty pDEST-GBKT7 and pDEST-GADKT7 vectors were used as negative control ...
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bioRxiv - Biochemistry 2023Quote: ... and a fusion construct containing nucleotides of CPY (1-50) and Atg15ΔN35 was generated by ligation (Clontech). To generate pRS426-ATG15-3xFLAG (TPL003) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated overnight with 0.5 ml of Talon (immobilised metal affinity chromatography, IMAC) resin (Clontech) in the presence of 150 mM NaCl and 20 mM imidazole ...
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bioRxiv - Bioengineering 2023Quote: ... CCR5-KO donor rAAV6 virus was produced in HEK-293T cells and was purified with AAVpro Purification Kit (TakaRa, San Jose, CA, USA). Electroporation of the RNP complex was performed using the Lonza 4D-Nucleofector (Lonza Group Ltd ...
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bioRxiv - Biochemistry 2023Quote: ... Ex Taq polymerase (Takara Bio, catalog no. RR01CM) and the PCR1 primer pair listed below ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was collected by centrifugation at 35,000 x g for 30 min and incubated with TALON IMAC resin (Clontech) and 20mM imidazole at 4 °C overnight.
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bioRxiv - Neuroscience 2023Quote: ... DCX: doublecortin (C-18) goat polyclonal IgG (1:200, Takara Bio, Cat# 632496). NeuN ...
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bioRxiv - Neuroscience 2023Quote: ... and Living Colors® DsRed Polyclonal Antibody (1:200, Clontech). BrdU ...
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bioRxiv - Microbiology 2023Quote: ... GISAID ID: EPI ISL 408667) [30] were generated by PCR using PrimeSTAR GXL DNA polymerase (TAKARA, Cat# R050A). A linker fragment comprising hepatitis delta virus ribozyme ...
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bioRxiv - Microbiology 2023Quote: ... mRNA was reverse transcribed into cDNA using the PrimeScript cDNA synthesis kit (Takara). The following primer pairs were used for gene amplification ...
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bioRxiv - Biochemistry 2023Quote: ... EPHA2- FN2 and antigen-A were purified using His 60 Ni Superflow resin (Takara), whilst antigen-B was purified with rProteinA Sepharose (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... RNA was extracted and libraries prepared using SMARTer Stranded RNA-Seq Kits according the manufacturer’s protocols (Takara) by the Integrated Genomics Operation (IGO ...
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bioRxiv - Plant Biology 2023Quote: ... under the control of cauliflower mosaic virus 35S promoter in the binary vector pBI121 (Clontech, USA) was transferred into Agrobacterium tumefaciens strain GV3101 and used to transform Arabidopsis by floral dip method and tobacco plants by leaf disk method (Horsch et al. ...