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Citations for Takara Bio :
5251 - 5300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... before whole transcriptome amplification using Advantage 2 Polymerase (Clontech) using oligos that introduce Illumina Nextera Multiplex Identifier (MID ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were run on a Smart Cycler System (Takara). Ct values of the gene targets were normalized to GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... the Lenti-X embryonic kidney 293T cell line (Clontech) in DMEM (4.5 g/L D-glucose) ...
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bioRxiv - Cell Biology 2023Quote: ... the Lenti-X embryonic kidney 293T cell line (Clontech, no. 632180) in DMEM (4.5 g/L D-glucose) ...
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bioRxiv - Biochemistry 2023Quote: ... with In-Fusion assembly reagent (Takara Bio). The resulting plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... retroviral supernatant was added onto retronectin-coated (1:25; #T100B TaKaRa) non-tissue culture treated plates and centrifuged at 2000 ×g for 60 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Synthesis of cDNA and library preparation were performed using the SMARTer Universal Low Input RNA Kit for Sequencing (Clontech) and the Ion Plus Fragment Library Kit (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... Stem121 (Takara Y40410; 1:1000), and Stem123 (Takara Y40420 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human specific GFAP (hGFAP, TAKARA, Stem123, 1:1000), anti-AQP4 (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... Expression level of Venus and Venus-tagged arrestin-3 proteins was determined with anti-GFP JL-8 antibody (#632381, Takara Bio USA, San Jose, CA). The endogenous β-actin (loading control ...
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bioRxiv - Cell Biology 2023Quote: ... HRas V12-C184S] were cloned in the pEGFPC1 vector (Clontech) by restriction digestion-ligation method ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was quantified and 1.5 μg of RNA was used to prepare cDNA using PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio) and random hexamer primer ...
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bioRxiv - Cell Biology 2023Quote: ... Ligation was set up using T4 DNA ligase (Takara Bio.). proper nucleotide additions were made to the forward primer to maintain the EGFP ORF ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human specific cytoplasm (TAKARA, Stem121, 1:500), anti-TBR2 (Abcam ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) (Clontech), human interleukin-2 (IL-2 ...
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bioRxiv - Microbiology 2023Quote: ... then introduced between BamHI and EcoRI sites of pRetroX-Tight-Pur vector (Takara, 632105). Resulting plasmids containing the desired mutation were confirmed by DNA sequencing.
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bioRxiv - Plant Biology 2023Quote: ... The bisulfite-converted library was split between two 50 ul reactions and PCR amplified using the following conditions: 2.5 U of ExTaq DNA polymerase (Takara Bio), 5 μl of 10X Extaq reaction buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... pHelper plasmid (Takara Bio) and AAV transfer plasmids using TransIT-VirusGEN Transfection reagent (Mirus) ...
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bioRxiv - Molecular Biology 2023Quote: ... A repair plasmid to replace the coding sequence of SOS1 with an HXGPRT-mCherry construct was generated by amplifying the 5’ and 3’ UTRs of SOS1 (adjacent to the protospacers in the Cas9-sgRNA plasmid) using primers 81-84 and inserting the resulting fragments into the pTKO2 plasmid27 by In-Fusion cloning (Takara). Parasites were co-transfected with both the Cas9-sgRNA and pTKO2 plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was incubated for 16 hr in rabbit anti-DsRed (Clontech; 1:5000) and mouse anti-TH antibodies (Immunostar ...
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bioRxiv - Cell Biology 2023Quote: ... pEGFP-hEB3 was transfected with the Trans-IT LT1 reagent (Takara) into wild-type or CEP290-KO cells seeded in a glass-bottomed dish (Matsunami Glass Ind.) ...
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bioRxiv - Cell Biology 2023Quote: ... and the PCR products were cloned into the EcoRI/SalI multicloning sites of the pEGFP-N3 vector (Takara), designated as pEGFP-hEB3 ...
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bioRxiv - Immunology 2023Quote: ... VSV-G-pseudotyped retrovirus was produced in GP2–293 packaging cells (Clontech) and VSV-G-pseudotyped lentivirus ...
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bioRxiv - Bioengineering 2023Quote: ... Complementary DNAs were generated utilizing the PrimeScript RT reagent kit (Takara) and then amplified using RT-PCR with specific primers ...
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bioRxiv - Cell Biology 2023Quote: ... the lentivirus were concentrated using Lenti-X concentrator (Takara Bio, catalog #: 631231) and quantified with the qPCR lentivirus titration kit (Abmgood ...
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bioRxiv - Cell Biology 2023Quote: ... Tet-On Inducible Expression System® plasmid (Clontech, Cat.No. 1. 634301) was linearized by digestion with EcoRI and AgeI ...
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bioRxiv - Cell Biology 2023Quote: ... the following combinations of gRNAs and complimentary adaptor ligated expression cassettes were transfected into the F1 mESCs using Xfect transfection kit (Takara, Cat.No.631317).
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bioRxiv - Cell Biology 2023Quote: ... the cDNAs were inserted into a pEGFP-C1 vector (Clontech cat#6084-1) using the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... Lenti-X™ 293T cells were obtained from Takara Bio (632180).
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bioRxiv - Cell Biology 2023Quote: ... concentrated down 10 or 30 times using the Lenti-X Concentrator (631232; Takara Bio). Supernatants were kept at -80°C prior to being directly applied to target cells which were then spun at 700 xg for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNAs were verified by sequencing and inserted into a bicistronic lentiviral expression vector encoding mCherry as a reporter (pLVX-EF1α-IRES-mCherry Vector, Clontech cat#631987). For GFP tagging ...
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bioRxiv - Cell Biology 2023Quote: ... neurons expressing the indicated constructs were incubated with or without 100 nM linker (AP21967; Takara Cat#635056) for 2 h prior to fixation and imaging.
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bioRxiv - Cell Biology 2023Quote: ... Fragments of interest were amplified using the EmeraldAmp GT PCR Master Mix (Takara, #RR310B) and intron- or exon - specific forward primers combined with a common exon junction-specific reverse primer ...
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bioRxiv - Cell Biology 2023Quote: ... All molecular cloning was conducted with the In-Fusion Assembly system (Takara #638948).
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed to detect expression of genes was using the SYBR® Premix Ex Taq kit (Takara, Japan) according to the manufacturer’s instructions and an iQ™5 real-time PCR System (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... and SYBR Green Premix Pro Taq HS (Takara). The primers used in this study were listed in the supplementary table 1.
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bioRxiv - Cell Biology 2023Quote: RT-qPCR was performed using PrimeScript™ RT Master Mix (Takara) and SYBR Green Premix Pro Taq HS (Takara) ...
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bioRxiv - Cell Biology 2023Quote: ... manuscript in preparation) The genetic sequence for H2B-mScarlet-I-DAAO and TOM20-mScarlet-I-DAAO was introduced by infusion cloning (Takara Bio) into a lentiviral backbone under control of a human EF-1α promoter and containing a puromycin selection cassette ...
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bioRxiv - Cell Biology 2023Quote: ... followed by qRT-PCR with SYBR green master mix kit (Takara). The reactions were performed in triplicate using the Agilent Mx3000P qPCR system and analysis was performed essentially as described earlier (Panigrahi et al. ...
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bioRxiv - Cell Biology 2023Quote: ... the EGFP sequence was PCR amplified from pEGFP-N1 (Clontech), and cloned in the pAIB-CAG lentiviral vector (a gift from Claudio Ballabio ...
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bioRxiv - Cell Biology 2023Quote: HEK293 (Clontech #C3003-1; lot #7030396) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-E-Cadherin (CDH1, Takara M108, 1:200), anti-GATA6 (R&D ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted and purified using NucleoSpin (Takara Bio, Shiga, Japan), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 5× PrimeScript™ RT mix (TaKaRa) was used to acquire cDNA ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA extraction was performed with RNAiso Plus reagent (TaKaRa, AKF0727A). Transcriptome library construction and subsequent sequencing analysis were completed by Novogene Technology Co. ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed using a TB Green®Premix Ex Taq™II (TaKaRa, Lot: AL51019A), and β-actin gene was used as a reference gene ...
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bioRxiv - Developmental Biology 2023Quote: ... total RNA was extracted from the target tissues (intestine, cerebral ganglion and ovary) at five developmental stages using RNAiso Plus reagent (TaKaRa, Lot: AKF0727A). cDNAs were synthesized from 1μg of total RNA (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... The H2afx coding sequence from Mus musculus was ordered from Eurofins and cloned into the pOZ-N-FH backbone adding the 1xHA tag at the N terminus using the in-fusion HD cloning kit (#12141, Takara). Full length wild type H2afx coding sequence was then mutagenized to obtain the desired S139A point mutation.
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bioRxiv - Cancer Biology 2023Quote: ... a reverse transcription kit (TaKaRa, Shiga, Japan) was applied to reverse transcribe 1000ng total RNA into cDNA ...
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bioRxiv - Cancer Biology 2023Quote: Retroviral plasmids were constructed in pMSCV (Clontech, Palo Alto, CA) vectors ...