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Citations for Takara Bio :
5201 - 5250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Physiology 2023Quote: ... Up to 30 ng of post-Immunoprecipitation dsDNA as measured by Invitrogen™ Qubit™ highsensitivity spectrofluorometric measurement was made into Illumina-compatible NGS libraries using the ThruPLEX® DNA-Seq kit from Takara Bio Inc ...
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bioRxiv - Neuroscience 2023Quote: ... the solution was replaced with a 0.5% BSA and 0.2% Triton X/PBS solution (PBST) containing a 1:1,000 dilution of Rabbit anti-DsRed (Takara, #632496) or Chicken Polyclonal anti-GFP antibody (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
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bioRxiv - Neuroscience 2023Quote: Each lysis plate well contained 4uL of lysis buffer (4U Recombinant RNase Inhibitor (Takara Bio 2313B), 0.05% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: PDGF-IRES-Cre was generated by cloning human PDGF-B and Cre into pQXIX vector (Clontech) as previously described5 ...
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bioRxiv - Cancer Biology 2023Quote: ... quantified with Lenti-X GoStix Plus (#631280, Takara Bio, Japan), aliquoted and stored at -80°C.
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNAs were analyzed using Premix Ex Taq (Takara) in qPCR experiments ...
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bioRxiv - Cell Biology 2023Quote: ... Clone and sequence using the pMD18-T vector (TaKara).
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bioRxiv - Cell Biology 2023Quote: ... which was then converted into cDNA using ReverTra Ace (Toyobo) and oligo-dT (Takara). The resulting cDNAs were analyzed using Premix Ex Taq (Takara ...
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bioRxiv - Biochemistry 2023Quote: ... was generated by amplifying full-length mouse ANGPTL4 cDNA (OpenBiosystems) and inserting it into a pCDNA6 vector using In-Fusion cloning (Clontech). A V5 tag was then appended to the C terminus of the open reading frame using Phusion site-directed mutagenesis (New England Biolabs).
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bioRxiv - Bioengineering 2023Quote: ... Using a BCA protein kit (TaKaRa, Shiga, Japan), a working solution (BCA reagent A ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were mixed with lentivirus at multiplicity of infection (MOI) equal to 5 in Retronectin (Takara, T100B)-coated culture plates and centrifuged at 1800 g for 1 h at 32 °C for lentiviral transduction before returning to normal culture condition ...
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bioRxiv - Bioengineering 2023Quote: ... The virus titer was determined using AAVPro titration kit (Takara).
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae was carried out by using the lithium acetate method of Yeastmaker™ Yeast Tansformation System (CLONTECH Laboratories, Inc.). The plasmid of pICASA was cleaved by XbaI and integrated into the chromosomal DNA of S ...
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bioRxiv - Bioengineering 2023Quote: ... DNA fragments were amplified with the high-fidelity polymerase PrimeSTAR Max DNA Polymerase from TaKaRa and colony PCR reactions were performed using DreamTaq polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... and concentrated using Lenti-X Concentrator (Takara Bio 631232). For CRISPR/Cas9 gene knockout ...
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bioRxiv - Cancer Biology 2023Quote: ... water-soluble tetrazolium salt (WST-1) (Takara, India). The western diet was customized and purchased from Research Diets Inc ...
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bioRxiv - Biochemistry 2023Quote: ... into which a PCR-amplified DNA fragment containing the mNeonGreen gene (Shaner et al., 2013) was inserted using the in-Fusion reaction (Clontech). To generate pRS426-CPY(1-50)-ATG15(Δ1-35)-mNeonGreen (YPL073) ...
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bioRxiv - Biochemistry 2023Quote: ... RNA were then used for library construction using a SMARTer Stranded RNA-seq kit (Takara, 634839) according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2023Quote: TssM193-490 coding region was obtained by gene synthesis (IDT) and cloned into pOPIN-K vector45 using the In-Fusion HD Cloning Kit (Takara Clontech). Point mutations were introduced using the QuikChange Lightning kit (Agilent Technologies).
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bioRxiv - Biochemistry 2023Quote: ... Unique clones were sub-cloned into the sAB expression vector pRH2.2 (kind gift of S. Sidhu) using the In-Fusion Cloning Kit (Takara Bio). The best candidates (based on highest ELISA signals ...
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bioRxiv - Biochemistry 2023Quote: ... The ZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech) in which the downstream EGFP gene was deleted and an HA tag was added at the C-terminus ...
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bioRxiv - Biochemistry 2023Quote: ... PrimeScriptTM RT Master Mix (TaKaRa) was used according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... the calreticulin signal sequence of pDsRed2-ER (Clontech) was removed by cleavage with NheI and BglII ...
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bioRxiv - Bioengineering 2023Quote: ... An RNA purification kit (NucleoSpin®) was purchased from TAKARA (Shiga, Japan). Dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cell Biology 2023Quote: ... All other constructs were generated by In-Fusion Cloning (Takara, Saint-Germain-en-Laye, France) using the manufacture’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... QPCRs was detected with SYBR Green PCR Master Mix (Takara, Japan) reagent ...
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bioRxiv - Immunology 2023Quote: ... total RNA underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), and amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems) ...
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bioRxiv - Genetics 2023Quote: DNA amplification was performed using PCR with PrimeSTAR GXL (Takara) using standard protocols and 35 cycles unless otherwise specified ...
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bioRxiv - Cell Biology 2023Quote: ... Library quantification was carried out using a next generation sequencing (NGS) library quantification kit from Takara Bio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 300 ng was used as template for the reverse transcription using the PrimeScriptTM RT Master mix (#RR036A, Takara, France). The qPCR was performed with a QuantStudio 6 Flex system equipped with a 384-well block and the SspAdvanced Unversal SYBR® Green Supermix (#1725272 ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA from these retained samples was reverse-transcribed into cDNA using 250 ng as a template and the PrimeScriptTM RT Master mix (#RR036A, Takara, France). To provide a comparison point for the allele-specific expression in F1 larvae ...
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bioRxiv - Developmental Biology 2023Quote: ... HpCTCF cDNA was amplified by RT-PCR with TaKaRa LA-taq (TaKaRa Bio Inc., Japan) using the following primers (5’-GTTCGCCATTCATTTGAGTGGATATTTC-3’ and 5’- CTGATCAGGTAAACTCTAAATCAGATTCC- 3’).
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bioRxiv - Developmental Biology 2023Quote: ... and LIF in a base medium of NDiff227 (Takara, Y40002) (Ying et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Sorted cells (>50,000 cells) were cultured on 48-well tissue culture plates coated with RetroNectin (r-fibronectin CH-296; Takara Bio Inc.) in EC media ...
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bioRxiv - Immunology 2023Quote: ... The fragments were gel purified and then assembled into a plasmid using in-fusion reagents (Takara Bio). The mCherry11-RNase L and mCherry1-10-RNase L were made similarly with oligos in Supplementary table 1 ...
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bioRxiv - Genomics 2023Quote: ... After treatment of DNase I (Takara Bio), the synthesized guide RNA was purified using the RNA Clean & Concentrator Kit (Zymo Research) ...
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bioRxiv - Genomics 2023Quote: ... purified RNA were constructed with the SMARTer Stranded RNA-seq Kit (Takara, cat. no. #634839) according to the manufacturer’s instructions for first- strand cDNA synthesis ...
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bioRxiv - Genomics 2023Quote: ... GenTLE Precipitation Carrier (Takara, #9094) and centrifuged for 15 min at 12000 r.p.m ...
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bioRxiv - Genomics 2023Quote: ... All PCR were performed using PrimeSTAR Max DNA polymerase (Takara, Japan). The DGP-4 fragments were then cloned into the AscI/NheI (NEB ...
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bioRxiv - Developmental Biology 2023Quote: K14-HA-Fzd6-tdTomato constructs were generated by using In-Fusion (Takara) cloning to first add the tdTomato tag from pBluescript (Stratagene ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-dsRed (1:500, Takara Bio), guinea pig anti-Sdk (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... A vector was constructed by fusing three PCR amplicons as described below using In-fusion cloning (Takara) and used for homologous recombination ...
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bioRxiv - Developmental Biology 2023Quote: ... and cloned into E.coli DH5α competent cells (Takara). Plasmids were extracted from bacterial cultured overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Nested PCR was performed with rTaq (Takara), followed by the PCR product fragments sequencing with Sanger sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... At 48 hpf the GFP + progeny was genotyped using a forward primer in the foxo1a endogenous locus and a reverse primer in the EGFP inserted transgene using a touch-down PCR with the Terra™ PCR Direct Polymerase Mix (639270; Takara). The PCR product was run in a 1% agarose gel to validate its size and the remaining product was sent for Sanger sequence.
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bioRxiv - Developmental Biology 2023Quote: ... and the cells were added with RNAiso Plus (Takara Bio, Japan) to digest ...
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bioRxiv - Developmental Biology 2023Quote: ... where the Hygromycin cassette was replaced by the Zeocin selection cassette using In-Fusion cloning (Takara). After one day of recovery ...
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bioRxiv - Developmental Biology 2023Quote: ... and inserted with the fragment of pBI-TRE (Clontech) containing the bidirectional tetracycline-responsive element (TRE ...