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Citations for Takara Bio :
5151 - 5200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed in a single-step using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara Bio). The HCoV-OC43 nucleocapsid gene was amplified with the following primers ...
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bioRxiv - Systems Biology 2023Quote: ... and cells were transfected with a transactivator rtTA plasmid (Clontech). After sorting ...
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bioRxiv - Systems Biology 2023Quote: ... In some cases lentivirus was concentrated using the LentiX Concentrator (Takara) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... or pGADT7 (AD) vector (Clontech, Mountain View, CA), respectively ...
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bioRxiv - Plant Biology 2023Quote: The full length cDNA of CLC2 was firstly cloned into a pMDTM20 T-vector (Takara Bio, San Jose, CA). The CLC2 containing AIM1 deleteion and AIM2 mutation as well as LDS mutated ATG8h were generated respectively by a Mut Express® II Fast Mutagenesis Kit V2 (Vazyme ...
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bioRxiv - Plant Biology 2023Quote: ... One base of 5’ dA overhang was added to the PCR amplicon of pMYB50 using Taq polymerase (Takara Bio Inc., Shiga, Japan), which was then cloned into pENTR5’-TOPO (Thermo Fischer Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293T (Takara Bio #632180), MCF10a (ATCC) ...
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bioRxiv - Systems Biology 2023Quote: ... those sgRNA-expressing cell lines were infected with 5x concentrated lentivirus (LentiX Concentrator, Takara) for the same dCas9 fusions ...
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bioRxiv - Systems Biology 2023Quote: ... This HEK293T reporter line was generated by transducing cells with lentivirus containing EGFP expressed from a pTRE3G (Clontech) promoter ...
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bioRxiv - Systems Biology 2023Quote: ... were delivered by spinfection into 100k cells using 1 mL of lentivirus that was concentrated 5x with LentiX Concentrator (Takara). 4 days later ...
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bioRxiv - Systems Biology 2023Quote: ... were delivered by spinfection into 100k cells using 1 mL of unconcentrated lentivirus or virus that was concentrated 5x with LentiX Concentrator (Takara). Blasticidin was added at day 3 or 5 to select for dCas9 delivery ...
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bioRxiv - Molecular Biology 2023Quote: ... SMARTer PCR cDNA synthesis kit (Clontech) was used according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was purified using NucleoSpin Gel and PCR Clean-up kit (Takara Bio). cDNAs of our interest were enriched by pulldown starting with 5-10 ug PCR synthesized cDNA and custom designed 5’ biotinylated oligonucleotide xGen Lockdown probes (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... MeSUFC and MeSUFDSU with specific primers for each gene using PrimeSTAR Max DNA Polymerase Premix (Clontech).
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing supernatants were first concentrated 10x using Lenti-X Concentrator (Takara, Cat# 631232). Single cell-suspended organoids were mixed with concentrated virus ...
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bioRxiv - Bioengineering 2023Quote: ... Quantitative RT-PCR was performed with TB Green Premix Ex Taq (Takara) using QuantStudio 1 system (Applied Biosystems) ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were incubated at 42 °C for 10 min with gDNA Eraser to remove the genomic DNA (Takara). PrimeScript RT Reagent Kit was used for RNA reverse transcription and cDNA synthesis ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... mRNA libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) (Clontech) and antibiotics ...
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bioRxiv - Immunology 2023Quote: ... Lactate dehydrogenase (LDH) in the supernatants from either cell type was quantified using the LDH Cytotoxicity Detection kit (TaKaRa or Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... a derivative of pInducer20-NA with the bidirectional doxycycline-inducible promoter from pTRE3G-BI (TaKaRa). Expression of VHH-HA and the C1C-EGFP inflammasome reporter was thus doxycycline-inducible ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized from RNA using the Prime script 1st-strand cDNA synthesis kit (Takara) as per the manufacturer’s instructions and using a mixture of random hexamer and oligo dT primers ...
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bioRxiv - Immunology 2023Quote: ... in 6-well plates pre-coated with 15 ug retronectin (Takara T100A). Plates were spinfected by centrifugation at 2500 rpm 32C for 90 minutes ...
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bioRxiv - Immunology 2023Quote: ... the puromycin selection cassette in pW-TH3 was replaced by a hygromycin selection cassette previously amplified from pMSCVhygro (Clontech). To generate pW-TH3 US18-V5 ...
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bioRxiv - Immunology 2023Quote: ... pIRES2-GFP was purchased from Clontech. CMVBL IRF3-5D codes for human IRF3 containing five amino acid substitutions (S396D ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was concentrated by evaporation using an Eppendorf Vacufuge system and was then added to lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara). Reverse transcription was performed followed by 20 cycles of PCR amplification to generate full length amplified cDNA ...
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bioRxiv - Immunology 2023Quote: ... The PCR protocol was executed using the Sapphire PCR master mix (TAKARA) following manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: HA-tagged TRIM34ΔSPRY constructs were cloned in pQCXIP (TaKaRa Bio, San Jose, CA, #631516) by SbfI (New England BioLabs #R3642S ...
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bioRxiv - Bioengineering 2023Quote: PCR reactions were performed using PrimeSTAR HS DNA Polymerase (Clontech) or Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... Stem101 (Takara Y40400; 1:100), Stem121 (Takara Y40410 ...
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bioRxiv - Bioengineering 2023Quote: ... SapphireAmp polymerase (Takara Bio) was used for yeast colony PCRs.
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bioRxiv - Microbiology 2023Quote: ... The RACE experiment was carried out using SMARTer RACE 5’/3’ Kit (Clontech) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated overnight at 4°C with primary antibody diluted in blocking buffer (these were: 1:200 Living Colors Ab, #632380, Takara; 1:100 L-plastin ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNA-seq libraries were prepared using a SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio) and sequenced on an Illumina NovaSeq 6000 using a NovaSeq S4 flow cell with 100 bp paired end reads by MedGenome ...
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bioRxiv - Molecular Biology 2023Quote: ... and then used as the template to synthesize double strand cDNA amplicons by PCR (optimal 17 – 21 cycles) using Advantage 2 PCR kit according to the manufacturer’s specifications (Clontech). cDNA was purified using NucleoSpin Gel and PCR Clean-up kit (Takara Bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... Captured cDNA was amplified using Takara LA Taq DNA polymerase Hot-Start version (Clontech) and purified using 1:1 (vol ...
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bioRxiv - Neuroscience 2023Quote: ... and then the coding sequence of eGFP (sequences extracted from pIRES2-EGFP, Clontech). Hβ4StrepTagII-nAChR-IRES-mTurquoise ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The reaction volume of 20 µL consisted of 10 µL of TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara, Japan), 0.8 µL each of gene-specific primers (10 µM) ...
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bioRxiv - Genetics 2023Quote: Lentiviruses were produced using the Lenti-X Tet-ON Advanced Inducible Expression System (Clontech), following the protocol described therein ...
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bioRxiv - Genetics 2023Quote: ... This resulted in the production of lentiviruses carrying either empty pLVX–Tight-Puro (Clontech) or pLVX–Tight-Puro–FoxM1-dNdK (Macedo et al ...
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bioRxiv - Genetics 2023Quote: ... as well as lentiviruses carrying pLVX–Tet-On Advanced (which expresses rtTA; Clontech). Fibroblasts were co-infected for 5-6 h with empty pLVX–Tight-Puro or FoxM1-dNdK and rtTA-expressing lentiviruses at 2:1 ratio ...
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bioRxiv - Genetics 2023Quote: ... Then One-step PrimeScript RT-PCR kit (Takara) was used to generate cDNA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we extracted DNA using NucleoBond HMW DNA (Takara Biotechnology, Japan) and eliminated the short fragments using the Short Read Eliminator Kit (PacBio) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR was performed using TaKaRa PrimeSTAR GXL DNA Polymerase following the manufacturer’s protocol (Takara Biotechnology, Japan). Amplified DNA fragments were purified by illustra ExoProStar (GE Healthcare Life Sciences) ...
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bioRxiv - Genetics 2023Quote: ... 1.25 U of Ex Taq DNA Polymerase (TaKaRa Bio Inc., Beijing, China) and DNA template - except for the negative (no-template ...
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bioRxiv - Genetics 2023Quote: ... and their size estimated using a 2000 bp-DNA ladder (TaKaRa Bio Inc., Beijing, China) as a reference and directly sequenced using primers MSP-3 and MSP-4B in separate reactions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and were applied to the SMART RACE kit (Takara Bio USA, Mountain View, CA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cloned into the PMD-19T vector (Takara, Japan) and then transformed into TOP10 Escherichia coli competent cells (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A total of six libraries were generated using the ThruPLEX DNA-seq 6S (12) kit (Takara Bio Europe, TOWN, France). The concentration of each sample pool was measured with Quant-iT PicoGreen dsDNA assay kit (Invitrogen Life Technologies ...