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Citations for Takara Bio :
5101 - 5150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: The Yeast Two-Hybrid assays were performed according to the Matchmaker Gold Yeast Two-Hybrid System (Cat#630489; Clontech) kit instructions ...
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bioRxiv - Biophysics 2023Quote: ... TALON resin (Clontech, USA), G-25 or PD-10 desalting column (GE Healthcare ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA polymerase was purchased from Takara Biochemicals (Shanghai ...
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bioRxiv - Biochemistry 2023Quote: The plasmids of EcOpgD and EcOpgG mutants were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was applied to TALON (Takara) affinity resin ...
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bioRxiv - Cell Biology 2023Quote: ... Products with 3’-dA overhangs were cloned into T-Vector pMD19 (Simple) (Takara Bio Inc., Shiga, Japan) to use as a template for sequencing.
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bioRxiv - Cell Biology 2023Quote: ... and a PrimeScript RTreagent kit with gDNA Eraser and SYBR Premix Ex Taq II (TliRNase H Plus) (TaKaRa) and an ABI7500 qPCR instrument (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... Viral particles were concentrated using Lenti-X Concentrator (Takara Bio, #631232), and cells were transduced at a high multiplicity of infection with different polybrene concentrations (8ug/mL-30ug/mL).
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bioRxiv - Developmental Biology 2023Quote: ... and the viral packing vectors (pCMV-ΔR8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284) ...
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bioRxiv - Neuroscience 2023Quote: pmCherry-1 (632525, Clontech) was used as the backbone and control vector for the overexpression experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA levels were measured using qRT-PCR kit (Takara) in combination with TB Green real-time PCR ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR was performed with SYBR Premix Ex Taq II (Takara, Dalian, China) using a StepOne (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: ... The transfected cells were cultured for 2 days and lentivirus were harvested and concentrated using the Lenti-X concentrator (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Plasmids were midi-prepped (Takara Bio), and BestGene ...
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bioRxiv - Bioengineering 2023Quote: ... The high fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio Inc., Japan) and the Hot start GoTaq polymerase (Promega Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... pTRE-Tight-Hygro was constructed by inserting the hygromycin-resistance gene into pTRE-Tight (Clontech). FLAG-αENaC was also subcloned into pCAGI-Hygro ...
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bioRxiv - Neuroscience 2023Quote: ... pEGFP-N2 and pmCherry-C1 (Clontech, Mountain View ...
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bioRxiv - Cell Biology 2023Quote: ... The baits were transformed into the strain Y2HGold and mated with the Universal Arabidopsis cDNA Library (Clontech) in Y187 ...
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bioRxiv - Microbiology 2023Quote: ... cDNAs were synthesized using PrimeScript RT reagent Kit (Takara, Dalian) and then diluted and subjected to quantitative PCR using TransStart Green qPCR SuperMix (TransGen Biotch ...
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bioRxiv - Plant Biology 2023Quote: Y2H analysis was performed using the Matchmaker® Gold Yeast Two-Hybrid System (Takara) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The OsGF14e gene was cloned into the pGADT7-Rec vector (Takara). The recombinant constructs were introduced into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 5 µL of 2.5 mM dNTPs (Takara #4025), with the following thermal cycle condition ...
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bioRxiv - Physiology 2023Quote: ... RNA was reverse-transcribed to cDNA (PrimeScript RT reagent kit, Takara Clontech) and quantitative real-time polymerase chain reaction (RT-PCR ...
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bioRxiv - Plant Biology 2023Quote: The 5’ and 3’ experiments were conducted with the use of SMARTer® RACE cDNA Amplification Kit (Takara Bio, United States, Cat# 634860) and Advantage Polymerase as described by Kruszka et al ...
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bioRxiv - Plant Biology 2023Quote: ... and SD4 (-Trp-Leu-His-Ade) media at 30℃ for 3–5 d according to the manufacturer’s manual (Clontech).
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bioRxiv - Plant Biology 2023Quote: ... and RT-qPCR was performed using SYBR Premix Ex Taq™ (TaKaRa, Dalian, China) by a Mastercycler ep Realplex (Eppendorf) ...
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bioRxiv - Plant Biology 2023Quote: ... fused with GAL4 activation domain was amplified and subcloned into the prey vector pGADT7 (Clontech Laboratories, Inc., Kusatsu, Japan). Competent yeast cells were prepared according to the Clontech Yeast protocols handbook using the Y1H Gold yeast strain ...
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bioRxiv - Plant Biology 2023Quote: ... The promoter sequence with and without mutation in ZDRE motif were artificially synthesized and cloned into the Kpn I-linearized pAbAi vector (Clontech Laboratories, Inc., Kusatsu, Japan) to generate the bait vectors ...
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bioRxiv - Plant Biology 2023Quote: ... the Y1H assays were performed following the manufacturer’s instruction (Clontech Laboratories, Inc., Kusatsu, Japan). The promoter sequence with and without mutation in ZDRE motif were artificially synthesized and cloned into the Kpn I-linearized pAbAi vector (Clontech Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Each sample (2 μg) of total RNA was used to synthesize first-strand cDNA with a PrimeScript RT Kit with gDNA eraser (Takara, RR0447A). RT-qPCR was performed using a Roche LightCycler 480 system with a SYBR Fast qPCR Kit (KAPA Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... using the In-Fusion® HD Cloning Kit (Takara Biomedical Technology Co. ...
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bioRxiv - Physiology 2023Quote: ... using In-Fusion Cloning (Takara Bio, Cat. No. 638911). Similarly ...
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bioRxiv - Neuroscience 2023Quote: ... LRP10 sequence-verified cDNA was subcloned from the pcDNA™3.1-LRP10-V5-His-TOPO® plasmid described previously (9) into the pLVX-EF1α-IRES-mCherry plasmid (Takara Bio, 631987). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... mCherry was introduced into the pcDNA™3.1-BspEI-LRP10 plasmid via PCR amplification from the pmCherryN1 plasmid (Clontech) via the addition of BspEI restriction sites in the primer sequences ...
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bioRxiv - Physiology 2023Quote: ... 1 ng of total RNA was used to amplify the cDNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara) following manufactureŕs instructions ...
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bioRxiv - Physiology 2023Quote: ... 1 ng of total RNA was used to amplify the cDNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara) following manufactureŕs instructions ...
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bioRxiv - Physiology 2023Quote: ... Complementary oligonucleotides with overhangs were annealed and cloned into the BbsI-digested U6b vector using a DNA ligation kit (Takara, 6023).
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bioRxiv - Physiology 2023Quote: ... Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus) (Takara bio RR820W) and a QuantStudio 6 Flex Real Time PCR system (ThermoFisher ...
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bioRxiv - Physiology 2023Quote: ... RNA was reverse-transcribed to cDNA (PrimeScript RT reagent kit, Takara Clontech) and quantitative real-time polymerase chain reaction (RT-PCR ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RNA was extracted from samples by Trizol reagent (TAKARA), reverse transcription was conducted by SuperScript II reverse transcriptase (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Preparations were then rinsed in a nuclei suspension buffer (NSB; made of PBS, 0.01%BSA and 0.1% RNAse inhibitor (Takara, cat# 2313A)) ...
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bioRxiv - Pathology 2023Quote: ... Expression levels of the genes were validated by quantitative real-time PCR analysis with SYBR Green (Takara Bio, CA, USA). Cycling parameters were 95°C for 20 seconds ...
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bioRxiv - Physiology 2023Quote: ... The cDNA was synthesized from 400 ng of DNase-treated total RNA by the Takara PrimeScript RT Reagent Kit with gDNA Eraser (Takara bio RR047B). Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were then constructed using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio Inc., Shiga, Japan), which features integration of unique molecular identifiers (UMIs) ...
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bioRxiv - Neuroscience 2023Quote: ... 12 cycles of amplification at PCR2 (final RNA-seq library amplification) and clean-up of final library using NucleoMag NGS Clean-up and Size Select beads (Takara Bio Inc., Shiga, Japan). Libraries were then quantified at the Genome Quebec Innovation Centre (Montreal ...
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bioRxiv - Molecular Biology 2023Quote: ... using TB Green Premix Ex TaqTM II (TaKaRa) with the following primers ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (Takara Bio).
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bioRxiv - Molecular Biology 2023Quote: ... in which the CMV promoter of pAAV-CMV vector (Takara Bio) was replaced by gfaABC1D ...
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bioRxiv - Molecular Biology 2023Quote: ... AAV-PHP.eB viruses were packaged in AAVpro 293T cells (Clontech). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins corresponding to the full-neck regions of myosin VIIIs were solubilized using 8 M urea and purified using a Ni-NTA His60 column (Takara), or for GST-tandem-IQ fusions ...