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Citations for Takara Bio :
5051 - 5100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... pEGFP-c2 from Clontech. GFP-LANA and LANA deletion plasmids were described previously 40,68 ...
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bioRxiv - Cell Biology 2023Quote: ... gel-purified (NucleoSpin Gel and PCR Clean-up kit from Takara) and the purified fragment was stored at -20°C ...
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bioRxiv - Immunology 2023Quote: ... 20ng of total RNA was used for the cDNA synthesis and amplification with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, cat#634889) according to instructions ...
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bioRxiv - Microbiology 2023Quote: ... the CPEC cloning assembly reaction was performed with the purified MotA-ASR insert and linearized vector using In-Fusion Snap Assembly master mix (Takara) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µl 5× In-Fusion Enzyme Mix (Takara) were mixed in a total of 5 µl H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... coli Stellar™ competent cells (Takara) were combined with 5 µl In- Fusion reaction product and incubated for 30 min on ice and subsequently heat shocked for 45 sec at 42 °C on a heating block ...
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bioRxiv - Molecular Biology 2023Quote: Using In-Fusion cloning (Takara), we exchanged the mScarlet of the reverse genetics’ plasmid pHW2000-PA-mScarlet (kindly provided by Prof ...
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bioRxiv - Microbiology 2023Quote: Amplification and detection were performed using the One Step PrimeScript™ RT-PCR Kit (RR064A, Takara Bio) with the following primers targeting the IAV M segment ...
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bioRxiv - Microbiology 2023Quote: ... filter plates were washed three times with sterile water using a vacuum apparatus (NucleoVac 96 vacuum manifold; Clontech Laboratories). The filter of well H12 was removed with a sterile pipette tip and tweezer ...
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bioRxiv - Cell Biology 2023Quote: ... Second strand synthesis and PCR amplification was performed by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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bioRxiv - Plant Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized using PrimeScript RT Master Mix (Takara Bio). RT-qPCR using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... or RNAiso Plus (Takara Bio) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... and reverse transcription was performed with PrimeScript RT Reagent Kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... into NheI/BamHI digested vector pEGFP-N3 (ClonTech) such that the encoded viral proteins are C-terminally fused to enhanced green fluorescent protein (EGFP) ...
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bioRxiv - Microbiology 2023Quote: ... of the resulting fragments were subjected to a CPER reaction in a 50 μl volume using 2 μl of PrimeStar GXL DNA polymerase (Takara Bio; #R050A). The following cycling conditions were used for CPER ...
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bioRxiv - Neuroscience 2023Quote: ... 1 ng total RNA was used for SMART-Seq HT PLUS (Takara Bio USA, Inc. Cat # R400748) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA isolation and cDNA synthesis were carried out using the SMARTer® Ultra® Low Input RNA Kit for Sequencing – v4 (Clontech, Cat.no. 634888). Libraries were generated from the resulting cDNA (0.2ng/ul per sample ...
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bioRxiv - Cell Biology 2023Quote: Hexa-Histidine (6×His)-tagged bacterial expression constructs were created using pColdI (Takara) vector backbone ...
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bioRxiv - Cell Biology 2023Quote: ... MSI2 ORF (NM_138962.2) was amplified by PCR with specific primers and high-fidelity Ex Taq DNA polymerase (Takara Bio USA, Inc., Mountain View, CA) using a cDNA containing human MSI2 obtained from OriGene (Rockville ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were prepared using the SMARTer Thruplex DNA-Seq kit (Takara), with each FFPE sample undergoing 7 PCR cycles ...
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bioRxiv - Immunology 2023Quote: ... viral particles were concentrated 20-fold to 400Lμl using Lenti-X Concentrator (Clontech, #631231, Mountain View, CA), and 100Lμl of concentrated virus was used to infect cells ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 400 ng of RNA was used to prepare cDNA using Primescript RT Master Mix (Clontech) at 42 °C following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PMLIII and PMLI isoforms were fused to the mRED vector (Clontech, Mountain View, CA, USA). TWIST1 ...
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bioRxiv - Cancer Biology 2023Quote: ... were fused to GFP-C (Clontech). mCherry LEGO-C2 was provided by Dr ...
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bioRxiv - Cell Biology 2023Quote: ... 70% confluent Lenti-X HEK293T (Clontech) cells were transfected in 10 cm2 dishes with 16.5 μg E3 sgRNA library plasmid ...
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bioRxiv - Evolutionary Biology 2023Quote: SARS-CoV-2 cDNA was generated from 100 ng of RNA in a RT-PCR reaction buffer containing 4 μL of 5X PrimeScript IV 1st strand cDNA Synthesis Mix (Takara Bio, Kusatsu, Shiga, Japan), 2 μL of 50 μM random hexamer primer ...
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bioRxiv - Systems Biology 2023Quote: ... 1.5 coverslips and the next day treated with 0.5 µM AP (Takara, 635057) for the indicated times where indicated ...
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bioRxiv - Systems Biology 2023Quote: ... heterodimerized with 0.5 µM AP21967 (Takara, 635057) on Day 6 ...
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bioRxiv - Plant Biology 2023Quote: ... with 150-bp paired-end (PE) reads by Takara Bio ...
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bioRxiv - Plant Biology 2023Quote: ... PCR was performed using Prime STAR MAX or Prime STAR GXL DNA Polymerase (Takara Bio). The plasmid pENTR1A (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and the 5′-end was phosphorylated with T4 polynucleotide kinase (Cat. # 2021S: Takara, Kyoto, Japan). This phosphorylated DNA fragment was ligated to the Bbs I cloning site in pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized using the SMART-Seq v4 Ultra Low input RNA Kit (Takara 634892). Sequencing libraries were made with the NEBNext Ultra II DNA Library Prep kit (New England Biolabs E7645L ...
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bioRxiv - Developmental Biology 2023Quote: ... Start codon mutant constructs were generated by mutation PCR using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Kusatsu, Japan) by replacing AUG with AAG at Met1 ...
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bioRxiv - Cancer Biology 2023Quote: Extracted total RNA with Trizol reagent was on the basis of the manufacturer’s instructions (Takara, Japan) in both tissues and cells ...
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bioRxiv - Cancer Biology 2023Quote: ... and was reversed into cDNA using PrimeScriptP RT kit (Perfect Real-Time; Takara). Then cDNA and SYBR Premium Ex Taq II were subjected to qRT-PCR reaction ...
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bioRxiv - Biophysics 2023Quote: ... Lysate was loaded on TALON resin (Takara Bio) gravity flow column ...
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bioRxiv - Cell Biology 2023Quote: ... Flow-sorted cells were cultured in ex vivo GT media in a 96 well flat bottom plate coated with 33.3 ug/ml of Retronectin (Takara). The 62 hr protocol consisted of pre-stimulation in GT media (24 hr ...
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bioRxiv - Microbiology 2023Quote: ... HA trimers were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... Fabs were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Where possible equivalent amounts of total RNA (100pg) from all samples were used for first strand synthesis with the SmartERv3 kit (Takara Clontech) followed by 15-18 cycles of amplification (according to manufacturers’ instruction) ...
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bioRxiv - Developmental Biology 2023Quote: ... TE cells were used as input to generate full length cDNA with the SMART-SeqTM v4 UltraTM Low Input RNA Kit (Clontech Laboratories, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... cDNA was obtained using the RNA to cDNA EcoDry Premix (639545, Takara). Quantitative PCR was performed with iQ SYBR Green Supermix (170-8884 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA amplification was performed using the SMART-Seq® v4 Ultra® low input RNA kit (Takara, cat. # 634891) with sixteen cycles of PCR ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... along with 0.5 μg pMSCVpuro vector (Clontech). After selection ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... BBQ and FICZ as indicated at 37°C in 96-well plates for 5 days prior to measuring viability using WST reagent (Takara) following the manufacturer’s protocol ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... concentrated with Lenti-X Concentrator (Takara 631231). CWR22Rv1 or C4-2b cells were infected with virus for 24 hrs in the presence of 8 μg/ml of polybrene in the growth medium ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviruses were made by transfecting Lenti-X 293T cells (Takara 632180) overnight with polyethylenimine (PEI) ...
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bioRxiv - Neuroscience 2023Quote: ... Rabbit anti-dsRed (1:500, Takara, 632496) and mouse anti-FasIII (1:20 ...
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bioRxiv - Genomics 2023Quote: ... 100 ng to 1 µg of total RNA was used for Hi-Mammalian whole transcriptome preparation (Takara Bio) and sequencing was performed on Nextseq2000 instrument with 1 x 72 bp single-end setup ...
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bioRxiv - Microbiology 2023Quote: ... Clarified lysates were incubated with TALON Metal Affinity Resin cobalt beads (Takara), and beads were then washed with Tris-HCL (pH 8.0 ...