Labshake search
Citations for Takara Bio :
5001 - 5050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted and purified using NucleoSpin (Takara Bio, Shiga, Japan), following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... 5× PrimeScript™ RT mix (TaKaRa) was used to acquire cDNA ...
-
bioRxiv - Developmental Biology 2023Quote: ... RNA extraction was performed with RNAiso Plus reagent (TaKaRa, AKF0727A). Transcriptome library construction and subsequent sequencing analysis were completed by Novogene Technology Co. ...
-
bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed using a TB Green®Premix Ex Taq™II (TaKaRa, Lot: AL51019A), and β-actin gene was used as a reference gene ...
-
bioRxiv - Developmental Biology 2023Quote: ... total RNA was extracted from the target tissues (intestine, cerebral ganglion and ovary) at five developmental stages using RNAiso Plus reagent (TaKaRa, Lot: AKF0727A). cDNAs were synthesized from 1μg of total RNA (ThermoFisher ...
-
bioRxiv - Cancer Biology 2023Quote: ... The H2afx coding sequence from Mus musculus was ordered from Eurofins and cloned into the pOZ-N-FH backbone adding the 1xHA tag at the N terminus using the in-fusion HD cloning kit (#12141, Takara). Full length wild type H2afx coding sequence was then mutagenized to obtain the desired S139A point mutation.
-
bioRxiv - Cancer Biology 2023Quote: ... a reverse transcription kit (TaKaRa, Shiga, Japan) was applied to reverse transcribe 1000ng total RNA into cDNA ...
-
bioRxiv - Cancer Biology 2023Quote: Retroviral plasmids were constructed in pMSCV (Clontech, Palo Alto, CA) vectors ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 ng of total RNA was reverse transcribed with the cDNA synthesis kit (Takara Bio Inc.) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA synthesis was performed using PrimeScript RT Reagent Kit (Takara RR037A). The qPCR reactions were carried out using Power SYBR Green PCR Master Mix (Applied Biosystems 4367659 ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse transcribed to cDNA using PrimeScriptTM RT reagent kit (TaKaRa, RR037A) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... SYBR dyes (Takara, Shiga, Japan) were combined into a 12ul system for cDNA amplification and signal collection ...
-
bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared for sequencing using the SMARTer Stranded Total RNA-seq Kit v2 (cat# 634413) – Pico Input Mammalian (Takara Bio USA). RNA samples were first synthesized into cDNA ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA was then subjected to the real time PCR for specific gene target by TB Green Premix Ex Taq (TaKaRa, RR420B) according to manufacturer’s instructions using Real Time PCR system (SIS-PCR005 ...
-
bioRxiv - Cancer Biology 2023Quote: ... using the Lenti-X Concentrator (Takara Bio) to achieve a 50x virus concentration ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using the SMARTer procedure through the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) for Illumina Sequencing ...
-
bioRxiv - Cancer Biology 2023Quote: ... and EF1a_TetOn3G (Clontech) and serially sorted to derive populations that were uniformly GFP-negative before and GFP-positive after doxycycline induction ...
-
bioRxiv - Cancer Biology 2023Quote: ... using an In-Fusion HD Cloning Plus kit (Takara Bio, Shiga, Japan); (iv ...
-
bioRxiv - Cancer Biology 2023Quote: ... libraries were prepared using the ThruPLEX®DNA-Seq kit (Takara, R400676) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2023Quote: ... sequencing libraries were generated (Takara SMARTer® ThruPLEX® DNA-seq Kit following the manufacture’s protocol) ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA was then synthesized with cDNA Synthesis Kit (Takara) according to the manufacturer’s protocols ...
-
bioRxiv - Cancer Biology 2023Quote: BON1/HA-Bcl-xL/TR-shCtBP2 and BON1/HA-Bcl-xL/TR-shRLuc #713 cells were treated with 1 µg/ml (before sorting) or 0.5 µg/ml (after sorting) doxycycline (Clontech Laboratories, Inc., 631311) for 96 hours ...
-
bioRxiv - Immunology 2023Quote: Primary antibodies used for imaging include Living Colors anti-DsRed Rabbit Polyclonal Pan Antibody (1:500; TaKaRa), Chicken Polyclonal anti-GFP (1:300 ...
-
bioRxiv - Cancer Biology 2023Quote: ... CT-2AZsGreen cells were generated using lentiviral particles derived from a pLVX-ZsGreen1C1 vector (Takara Bio #632566). All cell lines were grown in DMEM high glucose with 10% FBS (Gibco #16140-071 ...
-
bioRxiv - Immunology 2023Quote: ... 1x106 PBMCs were plated on retronectin (Takara Clonetech; T100B) on 6 well plates (Corning ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The linear vector pET-28a was generated by digestion with BamHI and XhoI (TaKaRa, Dalian, China). Recombinant plasmids were then transfected into E ...
-
bioRxiv - Microbiology 2023Quote: ... Genotyping PCR reactions were performed on 10ng of isolated parasite DNA using CloneAmp HiFi polymerase premix (Takara) in an Applied Biosystems thermocycler according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: ... The sequencing library was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit with nonstranded and polyA+ selection (Clontech, Takara Bio USA ...
-
bioRxiv - Microbiology 2023Quote: ... The His-tagged CRONE protein was purified by cobalt affinity chromatography using TALON® metal affinity resin (Takara) under native conditions in phosphate buffered saline (PBS) ...
-
bioRxiv - Microbiology 2023Quote: ... All PCR reactions described were performed with CloneAmp HiFi polymerase (Takara) and the specified primers in an Applied Biosystems Thermocycler according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... and quantified by RT‒qPCR using Primer/Probe N2 (NIHON GENE RESEARCH LABORATORIES, INC. Miyagi, Japan) and One Step PrimeScript™ III RT‒qPCR Mix (TaKaRa) with CFX96 (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... coli Stellar Competent cells (Clontech) and selected on carbenicillin plates ...
-
bioRxiv - Microbiology 2023Quote: ... and ligated with a DNA ligation kit (Takara). The constructs expressing GFP-BDI-bK6A and single amino acid substitutions in the SLiM sequence were generated by PCR-based site-directed mutagenesis using oligonucleotides and templates listed in Table S1 and inserted into the SalI site of pHE-C or phsp-GFP-Halo as described above ...
-
bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed using 500 ng of RNA in 10 µl volume of Solution with PrimeScript RT (TAKARA) and random hexamer primers ...
-
bioRxiv - Microbiology 2023Quote: ... The cleared lysate was loaded onto a 25 mL free-flow gravity column (GeneFlow) packed with 3 ml TALON® Metal Affinity Resin (Takara Bio), washed with 10 column volumes (CV ...
-
bioRxiv - Microbiology 2023Quote: ... siRNA Knockdown efficiency was tested as follows: 200 ng of RNA was reverse transcribed with Prime Script RT Master Mix (Takara) and quantified using QuantiFast SYBR Green RT-PCR Kit (Qiagen ...
-
bioRxiv - Microbiology 2023Quote: ... coli Stellar cells (Clontech) and plated onto Luria Bertani agar supplemented with 25 µg/ml chloramphenicol ...
-
bioRxiv - Immunology 2023Quote: ... Lenti-X 293T cells (Takara) were seeded in 15-cm2 dishes at a density of 10 × 106 cells per dish and the following day were transfected with 25 µg of spike expression plasmid with TransIT-Lenti (Mirus ...
-
bioRxiv - Microbiology 2023Quote: ... the V5-tag sequence followed by a Stop codon was introduced upstream of the IRES in the pLVX-IRES-Neo vector (Clontech, Addgene), using annealed oligo-cloning ...
-
bioRxiv - Molecular Biology 2023Quote: ... The medium was exchanged to CELLBANKER 1 plus (TaKaRa) and stored at −80°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were ordered from Takara (#632180). U2OS and ARPE19 cells were ordered from ATCC (HTB-96 and CRL-2302 respectively) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Total RNA (1 μg) was reverse transcribed with the PrimeScript™RT Reagent Kit (Takara Bio Inc.) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: Total RNA from human liver and fetal brain was purchased from Clontech Laboratories ...
-
bioRxiv - Molecular Biology 2023Quote: ... Total RNA was then extracted using a NucleoSpin® RNA Plus kit (Takara Bio) and subjected to cDNA synthesis and real-time PCR as described above ...
-
bioRxiv - Molecular Biology 2023Quote: ... and ORF66-TurboID were subcloned into the BamHI and NotI sites of pcDNA4 3xHA (C-term) using InFusion cloning (Clontech). TurboID-ORF34 was subcloned into the NotI and XhoI sites of N-term 3xHA pcDNA4 ...
-
bioRxiv - Cell Biology 2023Quote: ... and rabbit anti-dsRed (Clontech, dilution 1:200) as well as a directly Atto-565 conjugated anti-RFP nanobody (NanoTag ...
-
bioRxiv - Bioengineering 2023Quote: ... 1x volume of Lenti-X concentrator (Takara Bio) was combined with 3x volumes of clarified viral media and stored at 4°C overnight ...
-
bioRxiv - Bioengineering 2023Quote: ... Virus was titered using Lenti-X GoStix Plus (Takara Bio). Lentivirus encoding GFP was purchased by VectorBuilder (Chicago ...
-
bioRxiv - Molecular Biology 2023Quote: ... cDNAs were synthesized from these RNAs as templates using PrimeScript RT Reagent (Takara Bio). Expression levels of the respective genes of interest were measured using LightCycler 480 II system (Roche) ...