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Citations for Takara Bio :
4951 - 5000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... the total RNA was converted into cDNA using Prime Script RT Reagents (Takara). In brief ...
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bioRxiv - Genomics 2023Quote: ... BstI P-digested lambda phage gDNA was purchased from TaKaRa® (Cat ...
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the supernatant was loaded onto a 5 ml column with prewashed Talon resin (Clontech Laboratories, Inc, CA). The column was washed three times with the low pH buffer (pH 6.3) ...
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bioRxiv - Genetics 2023Quote: ... the two PCR fragments could be assembled and cloned into the SacII-NotI site of pPGKneo-F2F using the In-Fusion Cloning HD Kit (Takara; 639648), resulting in the generation of pPGKneo-F2F-Klf2-5HR ...
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bioRxiv - Genomics 2023Quote: ... were used for the genomic DNA (gDNA) extraction with NucleoBond AXG 100 or AXG 500 with NucleoBond Buffer Set IV (Clontech) or DNeasy Blood and Tissue kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the blot was developed with Western BLoT Chemiluminescence HRP Substrate (Takara, Kusatsu, Shiga, Japan), and the signals were detected using the ChemiDoc XRS+ Imager (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted using the NucleoSpin RNA Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Up to 5 μl of assembly reactions were used for heat-shock transformation of Stellar™ Competent Cells (Takara Bio).
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bioRxiv - Cell Biology 2023Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan).
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bioRxiv - Cell Biology 2023Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan).
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed with a Takara Thermal Cycler Dice® Touch (Takara Bio, Kusatsu, Japan). The RT-PCR program was as follows ...
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bioRxiv - Cell Biology 2023Quote: ... Lentivirus was produced by transfecting the plasmids in HEK293T cells using Lenti-X™ Packaging Single Shots (Takara Bio, Kusatsu, Shiga, Japan, #631278). The media containing lentivirus were collected from transfected cells and were further concentrated by 50-fold using Lenti-X Concentrator (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with D/D-Solubilizer (5 μM, Clonetech/Takara) and Cycloheximide (35.54 μM ...
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bioRxiv - Cell Biology 2023Quote: Human cardiomyocyte cells were purchased from Takara Bio (ref Y10060) ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was retro-transcribed by Takara kit (Takara Bio) ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfections were performed either with Xfect transfection reagent (Takara, 631318) or with Mirus Bio TransIT-2020 transfection reagent (MIR 5400 ...
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bioRxiv - Cell Biology 2023Quote: ... Complementary DNA was synthesized with PrimeScript™ RT Reagent Kit (#RR037A, Takara) at 37°C for 15Lmin and de-activated at 85°C for 5 sec ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was retro-transcribed by Takara kit (Takara Bio), GAPDH was used as internal reference ...
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bioRxiv - Cell Biology 2023Quote: ... and N-SREBP2 (1440 bps) were amplified using high-fidelity PCR (Advantage-HF 2 PCR kit, #639123; Clontech) from human pcDNA3.1-2xFLAG-SREBP1a (#26801 ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 2 μL of cDNA product was used for subsequent RT–qPCR analysis using SYBR1 Premix Ex Taq (Takara, Dalian, Japan). All primers used in this study are shown in Table S1.
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bioRxiv - Cell Biology 2023Quote: pmCherry-C1 (cat. No. 632524) and pAcGFP-N1 (cat. No. 632469) were purchased from Clontech. dsRed-EEA1-FYVE was a generous gift from Dr ...
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bioRxiv - Immunology 2023Quote: ... and then ten nanograms of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Strand specific RNA-seq libraries were then constructed using the SMARTer Stranded RNA-Seq Kit (Clontech #634839), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested and further concentrated using a Lenti-X Concentrator (Takara Bio Inc., Otsu, Japan). Naive CD4+ T cells were transduced with lentiviruses and polybrene (8 ug/mL ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...
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bioRxiv - Immunology 2023Quote: ... A total of 100 ng isolated RNA was used for cDNA synthesis using PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). RT-qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus ...
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bioRxiv - Plant Biology 2023Quote: ... and RNA-seq analysis (150 bp PE reads, Novaseq 6000 platform, by Takara Bio; Table S2) as described above.
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bioRxiv - Plant Biology 2023Quote: First-strand cDNA was synthesized from purified total RNA using PrimeScript RT Master Mix (Perfect Real Time; Takara Bio, Shiga, Japan) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: The WST-1 assay (Takara) was performed on HepG2 cells and Huh7 cells to assess the cellular cytotoxicity of Tecomella undulata ...
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bioRxiv - Biochemistry 2023Quote: Khc FL for in vitro studies was cloned with a HisSUMO-SNAP-3C-tag into MCS1 between BamHI and HindIII sites of the pFastBacDual vector by InFusion cloning (Takara). The Khc mutant constructs used for in vitro studies were generated by site-directed mutagenesis of the Khc FL plasmid using primers that either inserted a stop codon after aa 910 (Khc910 ...
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bioRxiv - Cell Biology 2023Quote: ... Separate populations were stably-transfected to express MLCK1-EGFP constitutively and mCherry-Ig3 inducibly via a Tet-on3G system (Clontech). Cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... BsrGI digested and ligated into a likewise digested C1 (Clontech) backbone ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were transformed into Y1H gold strain (Takara). The transformed yeast strains were grown on SD/-Leu and SD/-Leu/AureobasidinA media (Takara ...
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bioRxiv - Plant Biology 2023Quote: The PCR amplified promoter sequence of AtPHT1;5 gene was cloned into pAbAi vector (Takara) between the SacI and KpnI RE sites following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and AtMYB62 genes were amplified by PCR using gene specific primers and cloned into pGADT7-Rec vector (Takara). The recombinant constructs were transformed into Y1H gold strain (Takara) ...
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bioRxiv - Plant Biology 2023Quote: ... and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The transformed yeast strains were grown on SD/-Leu and SD/-Leu/AureobasidinA media (Takara) and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara ...
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bioRxiv - Genomics 2023Quote: ... Primers were designed with around 15 bp of overlapping sequence to ensure proper circularization with the In-Fusion HD cloning plus kit (Takara #638909). Mach1 competent cells (Thermo Fisher Scientific #C862003 ...
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bioRxiv - Microbiology 2023Quote: ... The product was circularized with In-Fusion HD Cloning (Takara Bio) as per the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... which was reverse transcribed to cDNA by Takara PrimeScript RT reagent kit (RR0036A ...
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bioRxiv - Molecular Biology 2023Quote: ... doxycycline (Clontech 631311), hygromycin B Gold (InvioGen) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 million Lenti-X HEK293T cells (Takara Bio, cat. no. 632180) were seeded in 2 mL DMEM (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 μg/ml doxycycline (Clontech, cat. no. 631311). Neuronal induction media was changed once a day for 2 more days ...
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bioRxiv - Molecular Biology 2023Quote: ... retrotranscribed using PrimeScript Reagent Kit (Takara) and amplified by RT-qPCR using PowerUp SYBR-Green MasterMix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: Human neurons were transfected on DIV28 with pEGFP-C1 (0.3 μg/well, Clontech) plus vectors expressing mCherry ...
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bioRxiv - Biochemistry 2023Quote: ... The digested XLone vector was then fused with the insert of the respective constructs by in-fusion cloning method according to the manufacturer’s instructions (Takara Bio Inc.). The resulting products from the respective in-fusion cloning reactions were transformed into stbl3 One-Shot competent cells according to the manufacturer’s instructions (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... For amplification of DNA sequences for cloning and NGS the PrimeSTAR Max DNA polymerase (Takara Bio Inc.) and for colony PCRs the OneTaq Quick-Load Master Mix Polymerase was used (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... and inserted into a modified pEGFP-N1 vector (Clontech) in which the downstream EGFP gene was deleted and an HA tag was added at the C-terminus ...
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bioRxiv - Biochemistry 2023Quote: ... the PrimeSTAR Max DNA Polymerase (Takara Bio Inc.) was used according to the manufacturer’s recommendation with a 25 µl reaction volume ...
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bioRxiv - Biochemistry 2023Quote: ... and reverse transcribed according to the manufacturer‘s protocol (Takara). qRT-PCR was carried out using SYBR Premi qRT-PCR ExTaq™ II (Takara ...