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Citations for Worthington Biochemical :
401 - 450 of 532 citations for Mouse Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... whole postnatal day (P) mouse forebrains were dissociated according to a modified papain-based protocol19 (Worthington), then filtered through a 40 μM filter before counting and dilution for scRNAseq ...
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bioRxiv - Cancer Biology 2024Quote: ... or a cocktail for mouse lung digestion composed of Collagenase Type I (CLS-1 LS004197, Worthington), Elastase (ESL LS002294 ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004197, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2021Quote: Tumor-bearing mice were sacrificed and tumors excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Cortices were then digested for 30 minutes at 37°C using a digestion medium containing papain (25 U/ml, Worthington Biochemical), DNAse I (2000 U/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Cortices were then digested for 30 minutes at 37°C using a digestion medium containing papain (25 U/ml, Worthington Biochemical), DNAse I (2000 U/ml ...
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bioRxiv - Microbiology 2022Quote: ... cut into small pieces and then digested for 30 minutes under gentle agitation at 37 °C with 7 mL of the following solution: type III collagenase (Worthington Biochemical), 4 mg/mL ...
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bioRxiv - Genomics 2019Quote: Nuclei from 5×105 cells were aliquoted into microcentrifuge tubes and incubated at 37°C for 5 min with varying amounts of DNase (0 to 75 U; Worthington, LS006344). Digestion was stopped by addition of an equal volume of termination buffer [10 mM Tris (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... following genotyping or embryonic day 18 embryos (rat) and dissociated enzymatically for 20 min at 37 °C in HBSS supplemented with 0.25% (w/v) trypsin (Worthington Cat# LS003707), followed by mechanical dissociation via trituration with fire-polished glass Pasteur pipettes ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were washed with PBS 48 hours after viral transduction and digested for 30 minutes at 37°C with a papain (Worthington, LS003119) solution (2mg/ml KnockOutTM DMEM without calcium ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Immunology 2019Quote: Tumour bearing mice were sacrificed and tumours were excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated in 37°C Trypsin-DNase (1g/L DNase-Worthington Biochemical-, 10g/L Trypsin - Worthington Biochemical-, 6mM NaOH in CMF-PBS) for 5 minutes and triturated 10x in 1ml RT DNase buffer (0.5g/L DNase ...
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bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
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bioRxiv - Cancer Biology 2022Quote: ... tissue samples were cut into small pieces and enzymatically digested for 30 min at 37 °C on a shaker with 5000U collagenase IV (Worthington, LS004188), 15 KU DNAse I (Sigma ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2023Quote: Tumor-bearing mice were sacrificed and tumors excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... urogenital ridges and adrenal glands were enzymatically dissociated at 37°C between 30 and 40 minutes using the Papain dissociation system (Worthington LK003150). Cells were resuspended in DMEM (Gibco 11885084 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2023Quote: The largest tumors from female C3-TAg and PyMT mice were minced and dissociated for up to 120 min at 37°C in a mixture of 1 mg/ml Collagenase (Worthington, LS004188), 20 U/ml DNase (Invitrogen ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... was dissected on ice and minced using two razor blades in a few drops of pre-warmed (37°C) Hibernate A medium containing 20 units/mL papain (Worthington LK003178). Minced tissue was incubated in 3.125 mL pre- warmed papain solution for 10 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... WAT depots from Scd1fl/fl mice were minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Bioengineering 2024Quote: ... hippocampi were dissected from brains of newborn pups (P0) and digested for 20 min at 37 °C with 10 units of papain (Worthington, USA), followed by gentle mechanical trituration ...
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bioRxiv - Neuroscience 2020Quote: ... The Papain Dissociation Kit (Cat# LK003150 Worthington) was used to dissociate the tissue ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Immunology 2019Quote: ... Specimens were finely minced between two scalpel blades and incubated for 15 minutes at 37°C with 600 U/mL collagenase IV (Worthington, Lakewood, NJ) and 20 ug/mL DNAse 1 (Roche ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
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bioRxiv - Neuroscience 2019Quote: ... neural retinas were digested at 37°C for 9 minutes in papain solution and single cells were obtained following manufacturer instructions (Papain Dissociation System, Worthington Biochemical, USA). Living cells were counted using a hemocytometer after trypan blue staining and resuspended in PBS at a density of 1×105 cells/μl ...
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bioRxiv - Neuroscience 2020Quote: Brain samples were minced with scissors and then incubated at 37 °C on a roller for 30 min in digest buffer containing 1.25 mg/mL Collagenase Type 4 (Worthington, Lakewood, NJ, USA) and 100 U/mL DNAseI (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... The hippocampal neurons were then dissociated via incubation at 37°C for 30 minutes in 2% papain solution (Worthington; catalog no. LS003127). Following incubation ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003127, 1 gm, $595.00 Ask -
bioRxiv - Genomics 2020Quote: ... Chromatin digestion was carried out at 30°C for 12 minutes with different concentrations of micrococcal nuclease (MNase, Worthington Biochemicals; cat# LS004798). The digestion was stopped by adding EDTA to final concentration of 10 mM ...
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bioRxiv - Systems Biology 2022Quote: ... testes were placed in a 15 ml conical tube containing 2 ml of prewarmed (35 °C) collagenase solution (1 mg/ml type I collagenase (Worthington, cat. # LS004196) and 60 U/ml Turbo Nuclease (Nacalai ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2021Quote: ... Cells were infected with lung homogenate supernatants at varying dilutions for 45 min at 37°C before the addition of Leibovitz L15 or MEM medium containing 0.9% agarose overlay containing Trypsin (Worthington Biochemical, NJ, USA), as previously described[54] ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and DNase I-treated for 30 min at 37°C (Worthington, 30U per 5 x 106 cells). After DNase I digestion ...
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bioRxiv - Molecular Biology 2022Quote: Five 10um cryostat slices of patient polyps were digested for 5 min at 37°C with a titration of MNase: 20 units/mL and 200 unit/mL of MNase (Worthington Biochemical Corp.) in MNase cleavage buffer (4 mM MgCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... which were immediately chopped and digested at 37°C with rotation in a solution containing 2mg/ml papain (Worthington Biochemical Corporation, LS003126) and 50 μg/ml DNase I in PBS ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Genomics 2023Quote: ... Tissue was coarsely chopped with a sterile scalpel and digested for 1hr at 34°C in a pH-equilibrated papain solution (Worthington, Lakewood, NJ) with constant CO2 flow over the enzyme solution ...
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bioRxiv - Neuroscience 2023Quote: ... Retinas were detached from the retinal pigmented epithelium and incubated for 15 min at 37°C in a solution containing papain (10 U/ml, Worthington; Lakewood, NJ) and L-Cysteine hydrochloride (∼1 mg/mL ...
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bioRxiv - Immunology 2023Quote: ... for 5 min at 4°C to remove circulating cells and then digested in RPMI 1640 with 1 mg/mL Type 2 collagenase (Worthington Biochemical LS004177) for 45 min at 37°C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... washed twice in HBSS and incubated during 30 min at 37°C in pre-warmed, oxygenated (95% O2, 5% CO2) Papain/DNase I (Worthington Biochemical Corporation) on a gyratory shaker (100 rpm) ...
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bioRxiv - Neuroscience 2021Quote: ... cerebellar tissue was dissected out from P6 mouse brains and digested using papain-dissociation system from Worthington following manufacturer’s protocol (Worthington Biochem ...
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bioRxiv - Microbiology 2019Quote: ... followed by a 10× dilution using 100 mM Tris-HCl (pH = 8.0) and tryptic digestion under 37°C for 18 hours using 1 µg of trypsin per well (Worthington Biochemical Corp., Lakewood, NJ). Digested peptides were desalted using a panel of lab-made 96-channel filter tips generated by inserting 96 20 µl filter tips into a 96-well cover mat and stacking each filter tip with 5 mg of 10-μm C18 column beads ...
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bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2022Quote: Cells were isolated from semitendinosus muscles of 10 Belgian Blue cattle (Supplementary Table 1) by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
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bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...
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bioRxiv - Cancer Biology 2022Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg mL-1 collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U mL-1 DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2023Quote: ... and then digested for 30 minutes under gentle agitation at 37°C with 7 mL of 4 mg/mL of type III collagenase (Worthington Biochemical, Lakewood, NJ) and 0.05 mg/mL of DNase I (Sigma ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... Brain tissues were manually minced using razor blades and enzymatically dissociated at 37°C for 30 min with 1 mg/mL collagenase type IV (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/mL DNase 1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg/ml collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/ml DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Neuroscience 2019Quote: Cortices from E15.5 mouse embryos were dissected followed by dissociation in complete Hank’s balanced salt solution (cHBSS) containing papain (Worthington) and DNase I (100ug/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... the mouse knee was minced into small fragments and digested with 3 mg/ml collagenase type I (Worthington) and 4 mg/ml dispase (Roche ...
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bioRxiv - Neuroscience 2019Quote: Cortices from E15.5 mouse embryos were dissected followed by dissociation in complete Hank’s balanced salt solution (cHBSS) containing papain (Worthington) and DNase I (100ug/mL ...