Collagenase, Type 4
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Suggested for pancreatic islet primary isolation. A dialyzed, lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004188 |
| CAS # | 9001.12.1 |
| Pricing | 1 gm, $215.00 |
| Product Code | CLS-4 |
| Activity/Specification | ≥160 units per mg dry weight |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Clostridium histolyticum |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mg/mL Collagenase Type IV (Worthington, LS004188), and 0.05 mg/mL Protease Type XIV (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Lungs were excised and processed by mincing with scissors followed by enzymatic digestion using 180 units/ml collagenase IV and 20 µg/ml DNase I (Worthington biochemical LS004188 and LS002139) in 5 ml RPMI incubated at 37°C for 30-45 mins in a shaker incubator before filtering through 70 µm filters and lysing RBCs with ACK lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... tissue was minced and incubated with collagenase type IV (600 U/ml, Worthington, LS004188) and dispase II (1.2 U/ml ...
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bioRxiv - Neuroscience 2022Quote: ... 100-18B)] and passaged twice a week with Collagenase IV (Worthington, LS004188).
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bioRxiv - Immunology 2021Quote: ... and 5 mg/mL collagenase IV (Cat. # LS004188, Worthington Biochemical) for 2.5 hrs at 37C ...
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bioRxiv - Developmental Biology 2019Quote: ... and collagenase type 4 (Cat. # LS004188, Worthington, Lakewood, NJ) for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... L4 and L5 DRGs were carefully collected on ice and digested with collagenase IV (0.2 mg/mL, Cat# LS004188, Worthington, NJ, USA) and dispase-II (3 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 0.4 mg/ml collagenase type 4 (Worthington LS004188) rotating at 150 rpm ...
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bioRxiv - Cancer Biology 2019Quote: ... 500 U/mL Collagenase IV (LS004188, Worthington), 100 U/mL Collagenase I (LS004196 ...
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bioRxiv - Cancer Biology 2020Quote: ... or 1000 U/mL collagenase type IV (Worthington, Cat: LS004188), and rotated for 30 minutes at 37 °C ...