Collagenase, Type 1
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase, clostripain and tryptic activities. Suggested for epithelial, liver, lung and adrenal primary cell isolations. A dialyzed, lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004194 |
| CAS # | 9001.12.1 |
| Pricing | 100 mg, $42.00 |
| Product Code | CLS-1 |
| Activity/Specification | ≥125 units per mg dry weight |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Clostridium histolyticum |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... and digested in 5 mL of PBS containing cold 1 mg/mL collagenase I (Worthington Biochemical LS004194), 3mg/mL dispase II (Roche 04942078001 ...
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bioRxiv - Neuroscience 2020Quote: ... and collagenase type I (3 mg/mL, Cat# LS004194, Worthington, Lakewood, NJ) and incubated for 60 minutes at 37°C under gentle agitation ...
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bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
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bioRxiv - Cancer Biology 2020Quote: ... and digested rotating for 30 minutes at 37°C with 1 mg/mL Collagenase I (Worthington Biochemical LS004194), 3mg/mL Dispase II (Roche 04942078001) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10mg/mL collagenase I (Worthington LS004194) and 10mg/mL DNase by constant rotation at 37C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... and collagenase type I (3 mg/mL, Cat# LS004194, Worthington, Lakewood, NJ) and incubated for 50 minutes at 37°C under gentle agitation ...
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bioRxiv - Developmental Biology 2022Quote: ... HBSS was removed and 2 ml of freshly made 1 mg/ml collagenase (Worthington Biochemical, LS004194) in HBSS was added to each tube ...
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bioRxiv - Immunology 2023Quote: ... and digested with 450U/ml Collagenase Type I (Worthington Biochemicals Cat # LS004194), 125U/ml Collagenase Type XI (Sigma Cat # C-7657) ...
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bioRxiv - Neuroscience 2023Quote: ... and digested at 37°C for 20 min with Collagenases-I and - IV (10 U/mL, 400 U/mL final, Worthington, Cat# LS004194, and LS004184) and DNase I (30 U/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Lungs from 4 mice were minced into ∼1 mm pieces using curved scissors and digested with 1 mg/ml collagenase type 1 (Worthington, cat# LS004194) and 1X dispase (Corning ...