Papain, Suspension
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full activity, the enzyme should be incubated in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS003127 |
| CAS # | 9001.73.4 |
| Pricing | 1 gm, $595.00 |
| Product Code | PAP |
| Activity/Specification | Activates to at least 20 units per mg protein |
| UNSPSC Code(s)* | 12352204.0 |
| Special Shipping/Handling | REQUIRES SPECIAL SHIPPING: ICE PACK |
| Source | Papaya Latex |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Neuroscience 2021Quote: ... and dissociated in HBSS containing 0.25% papain (LS003127, Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... The hippocampal neurons were then dissociated via incubation at 37°C for 30 minutes in 2% papain solution (Worthington; catalog no. LS003127). Following incubation ...
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bioRxiv - Cell Biology 2021Quote: ... and cells were dissociated by papain (Worthington, Cat# LS003127) digestion for 20 mins at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... “Activated papain solution” was prepared by mixing 8 U of papain (Worthington Biochemical, #LS003127) with 48 µl of “activator” containing H20 with 1.1 µM EDTA ...
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bioRxiv - Neuroscience 2024Quote: ... Papain Suspension (Worthington Biochemical Corporation, LS003127), DNase I (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... organoids (single organoids cultured in a 96-well plate or up to 8 organoids cultured in a 6-well plate) were incubated with an enzyme solution containing 10U/ml papain (Worthington, LS003127), 1x EBSS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Cortices were digested in papain (Worthington Biochemical Corporation LS003127) at 37°C for 45 min and triturated in DMEM supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was incubated in 34 U/mL papain (Worthington, #LS003127) and 40 μg/mL DNase I (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 200 units of papain (Worthington, LS003127), and 2.5 mM L-cysteine (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... and tissue was digested in papain solution (100 μl papain suspension (Worthington, Cat#LS003127) in 5 ml HBSS ...