Papain Dissociation System
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Use-tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LK003150 |
| CAS # | see components |
| Pricing | 1 bx, $295.00 |
| Storage Conditions | Store at 2-8°C. |
| Product Code | PDS |
| UNSPSC Code(s)* | 12161503, 12352204 |
Citations for this product:
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bioRxiv - Neuroscience 2021Quote: ... cells were dissociated using Papain Dissociation System (LK003150, Worthington). Dissociated cells were resuspended in a buffer containing 9.8 ml Hibernate A ...
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bioRxiv - Developmental Biology 2021Quote: Single-cell suspension was prepared from forebrains of P13 Stag2f/y or Stag2f/y;Cre pups using the Papain Dissociation System (Worthington Biochemical, LK003150) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Using a commercially available papain tissue dissociation kit (Worthington Biochemical, LK003150), hippocampi are transferred to Earle’s Balanced Salt Solution (EBSS)-based papain solution supplemented with DNase in a 6cm petri dish pre-equilibrated in 5% CO2 36°C incubator and chopped for 30 seconds with Bonn scissors before being returned to incubator for a 10-to 40-minute incubation commiserate with pup age ...
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bioRxiv - Neuroscience 2021Quote: ... and 250U ml−1 DNase in EBSS using the Papain Dissociation Kit (Worthington, Cat #:LK003150). The tissue was mechanically triturated and suspended cells were isolated and resuspended in EBSS with albumin-ovomucoid inhibitor solution ...
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bioRxiv - Developmental Biology 2020Quote: Prostate tissues were minced with scissors and then incubated in papain (20 units/ml) with 0.1 mg/ml DNase I (Worthington LK003150) at 37°C with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: Papain dissociation reagents were prepared according to manufacturer recommendations for the Papain Dissociation System (Worthington, #LK003150), with a slight modification ...
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bioRxiv - Genetics 2020Quote: ... the cells were resuspended in EBSS containing 50μg/mL of DNase I and 2 mg/mL ovomucoid (Worthington, LK003150). 0.5 volume of EBSS ...
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bioRxiv - Neuroscience 2021Quote: ... were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70μm and 40μm cell strainers (Falcon #07-201-431 and #07-201-430) ...
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bioRxiv - Genetics 2020Quote: ... cells were dissociated from transwells with a papain dissociation kit (Worthington Biochemical, Cat# LK003150) and filtered using a Flowmi cell strainer (Bel-Art SP Scienceware ...
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bioRxiv - Molecular Biology 2021Quote: ... iMNs were dissociated into single cells using the Papain Dissociation System (Worthington Biochemical, LK003150) after collection at Day 6 and resuspended to the concentration of 0.2 × 106 cells/mL with MN medium consisting of Neurobasal medium (Gibco 21103049 ...