Collagenase, Type 1
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase, clostripain and tryptic activities. Suggested for epithelial, liver, lung and adrenal primary cell isolations. A dialyzed, lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004196 |
| CAS # | 9001.12.1 |
| Pricing | 1 gm, $215.00 |
| Product Code | CLS-1 |
| Activity/Specification | ≥125 units per mg dry weight |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Clostridium histolyticum |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Immunology 2021Quote: Single cell suspensions were generated from Achilles tendons of MaFIA mice by enzymatic digestion with a solution containing 1 mg/mL collagenase I (Cat. # LS004196, Worthington Biochemical) and 5 mg/mL collagenase IV (Cat ...
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bioRxiv - Physiology 2020Quote: ... and then sequentially perfused with 0.2% collagenase type II (LS004196, Worthington) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Cell Biology 2021Quote: ... and placed in 2mg/mL collagenase (Worthington, LS004196) with 2% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2021Quote: ... Lungs were incubated in digestion buffer (5ml of DMEM supplemented with 5mg of collagenase I (Worthington LS004196), 10ul of 1M Ca2+ and 10ul of 1M Mg2+ ...
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bioRxiv - Microbiology 2022Quote: ... and 2.5 mg/mL Collagenase Type I (Worthington Biochem.com, cat#LS004196) at 37°C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was enzymatically dissociated with Collagenase type I (370 units/ml, LS004196, Worthington Biochemical) and DNase I (100 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... which consisted of HBSS containing Ca2+ and Mg2+ supplemented with 3.4 mg/ml collagenase type 1 (Worthington Biochemical Corporation LS004196) and 100 U/ml hyaluronidase (Sigma-Aldrich H3631) ...
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bioRxiv - Cancer Biology 2020Quote: ... Chopped tissue was digested in 100 U/mL Collagenase Type 1 (Worthington Biochemical Corporation, Cat# LS004196), 500 U/mL Collagenase Type 4 (Worthington Biochemical Corporation ...
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bioRxiv - Systems Biology 2022Quote: ... testes were placed in a 15 ml conical tube containing 2 ml of prewarmed (35 °C) collagenase solution (1 mg/ml type I collagenase (Worthington, cat. # LS004196) and 60 U/ml Turbo Nuclease (Nacalai ...
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bioRxiv - Genomics 2024Quote: ... we replaced the medium with 2 mL of digestive enzyme mixture I (3 mg/mL collagenase I (Worthington, cat. LS004196), 3 mg/mL collagenase II (Worthington ...