Papain, Suspension
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full activity, the enzyme should be incubated in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
Supplier | Worthington Biochemical |
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Product # | LS003126 |
CAS # | 9001.73.4 |
Pricing | 100 mg, $88.00 |
Product Code | PAP |
Activity/Specification | Activates to at least 20 units per mg protein |
UNSPSC Code(s)* | 12352204.0 |
Special Shipping/Handling | REQUIRES SPECIAL SHIPPING: ICE PACK |
Source | Papaya Latex |
Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Neuroscience 2020Quote: ... cortices were dissociated mechanically (using a scalpel blade) and enzymatically (using Papain: Worthington, LS003126) with various triturations and Nitex (Sefar ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue pellet was digested with a papain solution (0.7mg/ml papain suspension [LS003126; Worthington] ...
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bioRxiv - Neuroscience 2021Quote: ... and dissociated with papain (cat no. LS003126, Worthington Biochemical Corp., Lakewood NJ) and DNAse I (cat no ...
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bioRxiv - Neuroscience 2020Quote: ... The isolated hippocampi were treated with papain (Worthington, LS003126, Lot # 35N16202) in Hanks buffer with 10mM cysteine at 37°C for 15mins ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 20 units per mL papain (Worthington Biochemical LS003126) and 50 units per mL DNase (Worthington Biochemical ...
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bioRxiv - Biophysics 2019Quote: ... The isolated medullas were washed three times in three 150μL drops of enzyme solution containing (450 units/ml Papain (Worthington Biochemical #LS003126), 250 μg/ml BSA ...
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bioRxiv - Neuroscience 2022Quote: ... which contained 5 mL of 0.3 mg/mL papain (Worthington, LS003126) diluted in HBSS ...
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bioRxiv - Developmental Biology 2020Quote: ... For passaging the neurospheres were digested with papain (Worthington, LS003126) and mechanically dissociated into single cells ...
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bioRxiv - Neuroscience 2020Quote: ... 20 U/ml papain (Worthington, LS003126)) for 25-40 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Retinas were digested in papain 20U/ml (Worthington, CAT# LS003126), DNAseI 80U/ml (Sigma ...