PDS Kit, Papain Vial
A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1 mM L-cysteine with 0.5 mM EDTA.
Supplier | Worthington Biochemical |
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Product # | LK003176 |
CAS # | 9001.73.4 |
Pricing | 1 vi, $29.00 |
Product Code | PAP2 |
Activity/Specification | ≥100 units per vial |
UNSPSC Code(s)* | 12352204.0 |
Source | Papaya Latex |
Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Neuroscience 2021Quote: ... Neocortices were dissociated by the addition of papain (130 units, Worthington Biochemical, LK003176) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cerebral cortices were enzymatically digested using papain (20 U/ml, #LK003176, Worthington) supplemented with DNase I (250 U/ml ...
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bioRxiv - Bioengineering 2020Quote: Retinas were rapidly dissected and digested using a papain dissociation kit (catalog no. LK003176; Worthington Biochemical Corporation, Lakewood, NJ) following the manufacturer’s instructions to obtain a homogenous cell suspension ...
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bioRxiv - Neuroscience 2020Quote: ... The isolated cortical tissues were dissociated by papain dissociation kit (#LK003176, Worthington) supplemented with DNase I (250U/ml ...
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bioRxiv - Neuroscience 2020Quote: The mouse cortex between P0 and P2 was collected and dissociated with papain kit and DNase I (#LK003176, Worthington; 250U/ml; #D5025, Sigma). OPCs were purified by immnopanning approach according to our previous protocol (Lang et al. ...
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bioRxiv - Neuroscience 2021Quote: ... cerebral cortices were enzymatically digested using papain (20 U/ml, LK003176, Worthington) supplemented with DNase I (250 U/ml ...
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bioRxiv - Neuroscience 2020Quote: ... The mouse forebrain was dissociated using the papain dissociation system (LK003176, Worthington) and gentleMACS™ Dissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: The forebrain was dissociated by the papain dissociation kit (LK003176, Worthington) and dissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... PBS-perfused adult mouse brains were dissected and dissociated in 30-40U papain (Worthington, LK003176), 7.2 U dispase II (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi were dissociated using papain dissociation kit (Worthington LK003176) for 10 minutes at 30°C ...