Collagenase, Type 2
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and salivary primary cell isolation. Supplied as a dialyzed, lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004177 |
| CAS # | 9001.12.1 |
| Pricing | 5 gm, $900.00 |
| Product Code | CLS-2 |
| Activity/Specification | ≥125 units per mg dry weight |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Clostridium histolyticum |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Developmental Biology 2019Quote: ... Collagenase II (Worthington, LS004177) was next perfused into the heart for 10 min at 37 °C to dissociate cardiomyocytes ...
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bioRxiv - Cell Biology 2021Quote: ... and Glucose 5.5 mM) containing 0.05 mg/ml collagenase type II (Worthington, Cat# LS004177) and 1 mg/ml pancreatin (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: Lungs were extracted and placed into dissociation buffer that was composed of 1mg/ml collagenase type 2 (Worthington, #LS004177), 0.25U/ml neutral protease (Worthington ...
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bioRxiv - Paleontology 2019Quote: ... Hyaline articular cartilage was dissected and human chondrocytes isolated using 3 mg/mL Collagenase Type II (Worthington Cat # LS004177) in DMEM medium (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... Collagenase Type II (Worthington LS004177), Ethyl carbamate (Alfa Aesar AAA44804-18) ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 mg/mL collagenase type II (Worthington Biochemical Corporation; LS004177) (37) ...
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bioRxiv - Physiology 2020Quote: ... and digested for 75 mins at 37C in a collagenase solution (Collagenase type II, Worthington LS004177). (HBSS with 3% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... enzymatic digestion was achieved by 3 min of perfusion with calcium-free digestion buffer (400 units/mL of collagenase II in perfusion buffer; Worthington LS004177) followed by 12 min of perfusion with digestion buffer containing 50 μM CaCl2 ...
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bioRxiv - Physiology 2022Quote: ... and digested in 6 ml of 2-mg/mL type II collagenase (Worthington # LS004177) for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: Isolated mouse embryos were minced and digested in sorting medium (Ham’s F10 with 10% horse serum) containing 400U/ml Collagenase II (Worthington; LS004177) for 1h at 37 °C in a shaking water bath ...