Papain, Lyophilized
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To insure full activity, the enzyme should be incubated in a solution containing 1.1 mM EDTA, 0.067mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS003119 |
| CAS # | 9001.73.4 |
| Pricing | 100 mg, $100.00 |
| Product Code | PAPL |
| Activity/Specification | Activates to at least 15 units per mg protein |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Papaya latex |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Cancer Biology 2020Quote: ... tissue was enzymatically digested with 5 mL of papain digestion solution (0.94 mg/mL papain (Worthington #LS003119), 0.48 mM EDTA ...
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bioRxiv - Neuroscience 2020Quote: ... Digestive medium was freshly prepared by dissolving 12 mg papain powder (Worthington, cat. no. LS003119) in 6 ml hibernate without calcium (BrainBits ...
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bioRxiv - Neuroscience 2021Quote: ... 2–3 randomly selected hSS per hiPSC line were pooled in a 1.5-ml Eppendorf tube in the dissociation solution (10U/mL papain (Worthington Biochemical, LS003119), 1 mM EDTA ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was enzymatically digested using Papain (2 mg/ml, Cat No. LS003119, Worthington Chemicals), and cells dissociated into single cells using fire-polished glass pipettes ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... Ganglia were incubated at 37□C for 25 minutes in 1 mg/ml papain (LS003119; Worthington), followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176 ...
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bioRxiv - Cell Biology 2020Quote: ... Digestion was performed in RPMI media containing 20 U/mL papain (Worthington Biochemical LS003119) and 0.005% DNAase I ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was enzymatically digested using Papain (2 mg/ml, Cat No. LS003119, Worthington Chemicals), and cells dissociated into single cells using fire-polished glass pipettes ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were washed with PBS 48 hours after viral transduction and digested for 30 minutes at 37°C with a papain (Worthington, LS003119) solution (2mg/ml KnockOutTM DMEM without calcium ...
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bioRxiv - Neuroscience 2022Quote: ... Concentrations of enzymes: papain 20U/ml (Worthington LS003119), collagenase IV 1mg/ml (Worthington LS004188 ...
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bioRxiv - Neuroscience 2024Quote: Hippocampi were dissected from neonatal P0 homozygous KIBRA KO or PICK1 KO mice and incubated with 670 μg/mL Papain (Worthington #LS003119) and 100 μg/mL DNase (Sigma-Aldrich #DN-25 ...