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Citations for Worthington Biochemical :
201 - 250 of 310 citations for 7 Chloro 9 methyl 3 4 dihydro 2H benzo b oxepin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... we replaced the medium with 2 mL of digestive enzyme mixture I (3 mg/mL collagenase I (Worthington, cat. LS004196), 3 mg/mL collagenase II (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Genomics 2020Quote: ... Processing buffer (5% fetal bovine serum in PBS) and DNAse I (100 µg/ml, Worthington LS006344) were then added and the samples rocked at 37°C for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... cut in small pieces and incubated in PBS containing 5 mg/ml of papain (Worthington, LS003124), 0.24 mg/ml of L-cysteine ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003124, 25 mg, $40.00 Ask -
bioRxiv - Genomics 2023Quote: Processing buffer (5% fetal bovine serum in PBS) and DNase I (100 μg/mL, Worthington LS006344) were then added ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then DNAse treated with 4 ml dissagregation media (DMEM, 10% FBS, 100 μg/ml DNAse (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and the telencephalon was manually cut into pieces, followed by enzymatic digestion (DMEM GlutaMAX 1% P/S, 3% papain (Worthington 3126), 1.5% 10 mg ml−1 DNAse and 2.4% 12 mg ml−1 cystein ...
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bioRxiv - Immunology 2020Quote: ... inflated with a 1.5 ml mixture of 1 ml low melting agarose (3% w/v) and 500 μl 4U/ml dispase (Worthington Biochem, LS02104), and incubated for 1 h at room temperature (RT) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... enzymatic digestion was achieved by 3 min of perfusion with calcium-free digestion buffer (400 units/mL of collagenase II in perfusion buffer; Worthington LS004177) followed by 12 min of perfusion with digestion buffer containing 50 μM CaCl2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Biophysics 2022Quote: ... CaCl2 was added to a final concentration of 40 mM (21 µL of 1 M) followed by the addition of 75 U (3 µL of 25 U/µL) of micrococcal nuclease (MNase, Worthington Biochemical) and incubated at 37◦C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue was then washed with PBS and digested for 10 min at 37°C in 3 mg/ml collagenase II (Worthington, LS004176) in HBSS supplemented with 5% FBS and 10 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... Excised hearts were cannulated to the Langendorff retrograde perfusion system through the aorta and perfused with Base solution (Tyrode’s solution with 10 mM taurine and 12 mM 2,3-butanedione monoxime) and then digested with prewarmed Digestion buffer (Base solution with 180 U/mL Collagenase Type II (Worthington, LS004177) and 25 μM CaCl2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 5 minutes at RT in fetal calf serum complemented with DNase I (Worthington), followed by mechanical dissociation ...
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bioRxiv - Immunology 2021Quote: Perfused lungs were finely minced and digested in 5 mL of a 1.78mg/mL Collagenase IV (Worthington) solution in PBS (with Ca2+ ...
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bioRxiv - Cancer Biology 2020Quote: ... tissue was enzymatically digested with 5 mL of papain digestion solution (0.94 mg/mL papain (Worthington #LS003119), 0.48 mM EDTA ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... and digested in 5 mL of PBS containing cold 1 mg/mL collagenase I (Worthington Biochemical LS004194), 3mg/mL dispase II (Roche 04942078001 ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2020Quote: ... Mice were immunized as indicated in each Figure with 5 μg low-endotoxin OVA (Worthington Biochemical Corporation) together with 50 μg papain (P4762 ...
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bioRxiv - Physiology 2024Quote: ... bubbled with 95% O2/5% CO2) containing collagenase type I (1.5 mg/ml, Worthington, Lakewood, NJ, USA) and 1% bovine serum albumin ...
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bioRxiv - Immunology 2023Quote: ... Mice were immunized as indicated in each Figure with 5 µg low-endotoxin OVA (Worthington Biochemical Corporation) mixed with either 50 µg papain (P4762 ...
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bioRxiv - Neuroscience 2024Quote: ... The DRG pieces were incubated in 5 ml HBSS containing 2 mg/mL Stemzyme (LS004106, Worthington Biochemical) and DNAse I (LS001239 ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004106, 50 mg, $110.00 Ask -
bioRxiv - Immunology 2022Quote: ... biopsies were minced finely with dissection scissors and mixed with 3mL of digestion buffer (0.8mg/mL collagenase type 4 (Worthington) and 0.02mg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... the ventricle was then cut into four pieces and digested in PBS containing 4 mg/ml collagenase II (Worthington) with gentle shaking at 37°C until complete digestion ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mL of Ca2+/Mg2+-free HBSS containing 4 mg of collagenase type II and 1.25 mg of Dispase type II (Worthington) was added and incubated at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... for 4 minutes at a flow rate of 5ml/min followed by 0.8mg/ml Type 1 Collagenase (Worthington, UK) in Dulbecco Minimum Essential Medium (Gibco # 11965-092 ...
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Pulmonary infection interrupts acute cutaneous wound healing through disruption of chemokine signalsbioRxiv - Immunology 2020Quote: ... The tissue was incubated for 45 minutes at 37° C in DMEM containing type 4 collagenase (Worthington Biochemical Corporation) and DNAse I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
Product cited:
Highly polymerized; hyperchromicity ≥27%. A substrate for deoxyribonuclease assays. Prepared...Cat# LS002105, 100 mg, $35.00 Ask -
bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue from 2-3 hypothalamiwas pooled into 1-1.5 mL of HABG containing papain (20 u/mL, Worthington Biochemical, Lakewood, NJ, US). Tissue was broken into smaller pieces by pipetting 2-3 times with a P1000 pipet followed by a fire-polished glass pipette with a wide opening ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml, Worthington, Lakewood, NJ) and chondroitinase-ABC (1 U/ml ...
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bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
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bioRxiv - Cell Biology 2023Quote: ... Half of the samples were treated with a final concentration of 5 μg/mL bacterial collagenase (Worthington Biochemical) for 3 d at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Remaining intestinal pieces were then incubated in HBSS containing 5% FBS and 150U/ml collagenase type 2 (Worthington) at 37°C with stirring for 45min to isolate LP cells ...
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bioRxiv - Developmental Biology 2021Quote: ... The dissected hypothalamuses were further divided into 2-4 pieces before cell dissociation for single cell isolation using Papain Dissociation System (cat no. LK003150, Worthington Biochemical Corporation ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Immunology 2019Quote: ... DRGs were further digested with 1 mL of collagenase type II/dispase solution (HBSS/10 mM HEPES/4 mg/mL collagenase type II (Worthington)/5 mg/mL dispase (Thermo Fisher) ...
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bioRxiv - Bioengineering 2020Quote: ... acellular PCL and GA or GP crosslinked dMEP scaffolds (n = 4/group) were digested in 2 mg/mL collagenase type 2 solution (Worthington) for 24 h at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
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bioRxiv - Cell Biology 2019Quote: ... The muscles were digested in 4 ml:1 g of tissue wet weight in a solution of 0.5% Collagenase II (Worthington Biochemicals), 1.2 U/ml Dispase (Worthington Biochemicals) ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS and dissociated into single cells using collagenase type 4 enzyme at 200Units/ml (Worthington, Lakewood, NJ, US) for 10min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and enzymatically digested at 37°C for 80 min in dispase type II (4.7 mg/ml) plus collagenase type II (4 mg/ml) (Worthington Biochemical), as described previously [62] ...
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bioRxiv - Cell Biology 2024Quote: ... The muscles were digested in 4 ml:1 g of tissue wet weight in a solution of 0.5% Collagenase II (Worthington Biochemicals), 1.2 U/ml Dispase (Worthington Biochemicals) ...
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bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
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bioRxiv - Developmental Biology 2019Quote: ... and 65 mg of the minced tissue was placed in 2 ml digestion buffer containing 3 mg/mL Type 2 collagenase (Worthington, Collagenase Type 2), 1.5 mg/mL ProNase E (Sigma P6911) ...
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bioRxiv - Bioengineering 2020Quote: ... The scaffolds were finely minced with surgical scissors and digested in 1 mL of 4 mg/mL collagenase (Worthington Type I) in Kreb’s Ringer Buffer supplemented with 1% BSA ...
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bioRxiv - Immunology 2019Quote: ... and the GL261 tumors were excised and dissociated in Collagenase IV Cocktail (3.2 mg/mL Collagenase Type 4, 1.0 mg/mL deoxyribonuclease I, 2 mg/mL Soybean Trypsin Inhibitor [Worthington Biochemical, Lakewood, NJ]) for two 15 min intervals with gentle mixing at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... hindlimb muscles from 2-4-month-old mice were minced and digested in DMEM containing collagenase type II (0.2%; Worthington Biochemical Corporation) and dispase II (2.5 U/mL ...
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bioRxiv - Neuroscience 2022Quote: ... Nerves were minced with fine scissors and incubated in 1 ml PBS supplemented with collagenase (4 mg/ml Worthington Biochemical, LS004176) and dispase (2 mg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor samples were transferred into a 5mL Eppendorf tube containing 4.5mL cold enzymatic dissociation mix (100µg/mL Collagenase Type 4 (Worthington Biochemical Corporation #LS004186) and 10 µg/mL DNAse I (StemCell Technologies #07900 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Genetics 2023Quote: ... and perfused with a type 2 collagenase digestion buffer (2.4 mg/ml concentration) at a flow rate of 4 ml/min (Worthington Cat# LS004176). The large blood vessels and the atria were excised from the ventricles and minced in a stop buffer containing 10% calf serum ...
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bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...