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Citations for Worthington Biochemical :
251 - 300 of 310 citations for 7 Chloro 9 methyl 3 4 dihydro 2H benzo b oxepin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... pH adjusted to 7.6] and mechanically dissociated to single cells after adding ovomucoid (Worthington, 0.7 mg per 5 mice) and DNAse (Worthington ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in Dispase II (5 mg/mL; StemCell 7913) and DNase I (100 μg/mL; Worthington Biochemical LS002006) for 5 minutes ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002006, 20 mg, $96.00 Ask -
bioRxiv - Molecular Biology 2019Quote: ... This was achieved by treating the tissue pieces with 5 ml 1 mg/ml elastase (Worthington Biochemical Corporation LS002292) for 30 minutes at 37°C and then 5 ml collagenase added for a further 1-2 hours ...
Product cited:
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme...Cat# LS002292, 100 mg, $156.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... cells were centrifuged for 5 minutes and the pellet was resuspended with DNAase/ovomucoid inhibitor according to manufacturer’s protocol (Worthington). Cells were then centrifuged and resuspended on ice in Leibovit’z L-15 medium supplemented with 0.1% of bovine serum albumin ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryonic single cell suspensions from were obtained by performing eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (0.7mg/ml Worthington) at 37°C under agitation ...
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bioRxiv - Immunology 2023Quote: ... 15 mL of pre-warmed digestion solution (1X HBSS with Ca+ and Mg, 5% FBS, 50 U/ml Dispase II [Millipore-Sigma, cat # 4942078001], 0.5 mg/ml DNAse I [Worthington Biochemical ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... The nuclei suspension was preincubated at 37°C for 5 min and followed by micrococcal nuclease (Worthington, cat# LS004798) digestion was carried out by following the manufacturer’s protocol ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Physiology 2023Quote: ... The muscle layer was cut into pieces and incubated in 5 ml of digestive solution containing 1.5 mg collagenase (Worthington), 1 mg trypsin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... The remaining tissue was then incubated in 5 mL of digest solution [200 U/mL Collagenase IV (Worthington, LS004189) and 400 U/mL DNase I (Worthington ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Physiology 2020Quote: ... oocytes contained in 1-3 mL of ovary lobes extracted via survival surgery were isolated by enzymatic digestion with collagenase type II (Worthington Biochemical Corp., NJ, USA) under mechanical agitation ...
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bioRxiv - Cell Biology 2019Quote: ... Tissues were cut and minced with scissors and placed in 15 ml conical tubes containing digestion buffer (2 ml DPBS and Collagenase II at 3 mg/ml; Worthington Biochemical, Lakewood, NJ, USA) and incubated at 37 °C for 40 min with gentle shaking at 100rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Each tissue was cut and minced with scissors and then placed in 15 ml conical tubes containing digestion buffer (2 ml DPBS and Collagenase II at 3 mg/ml; Worthington Biochemical, Lakewood, NJ, USA) for 40 min of incubation at 37°C with gentle shaking at 100 rpm ...
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bioRxiv - Cell Biology 2019Quote: ... each consisting of calvaria from 6 pups were digested in 10 ml collagenase solution (1 mg/ml Collagenase type I and type II (1:3 ratio, Worthington Biochemical Corp., Lakewood, NJ), 1 mM CaCl2 ...
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bioRxiv - Cancer Biology 2024Quote: The ovarian cancer tissues were incubated in DMEM high glucose culture medium supplemented with 3 mg/ml collagenase D (Worthington-biochem, Lakewood, NJ, USA) at 37°C for 45 min ...
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bioRxiv - Neuroscience 2020Quote: Brain samples were minced with scissors and then incubated at 37 °C on a roller for 30 min in digest buffer containing 1.25 mg/mL Collagenase Type 4 (Worthington, Lakewood, NJ, USA) and 100 U/mL DNAseI (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: 30 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 60 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Immunology 2023Quote: ... for 5 min at 4°C to remove circulating cells and then digested in RPMI 1640 with 1 mg/mL Type 2 collagenase (Worthington Biochemical LS004177) for 45 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Prepontine regions from 5-6 dpf fish were dissected into Evans buffer and dissociated in 1 mg/ml neutral protease (Worthington) in Evans buffer for 30 min at room temperature with gentle shaking ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM MgCl2) and LC-MS/MS was performed by on-bead digestion with trypsin (TPCK-treated, Worthington Biochemical).
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Pluripotent Stem Cells Can Be Isolated from Human Peripheral Nerves after in vitro BMP-2 StimulationbioRxiv - Cell Biology 2019Quote: ... the minced nerve pieces were pelleted by centrifugation at 500xg for 5 minutes and followed by digestion in 0.2% (0.27 U/ml) collagenase (Worthington Biochemical Corp) at 37°C for 90 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were then centrifuged at 300*g for 5 minutes and the pellet resuspended in 1 mL of trypsin inhibitor (Worthington) plus DNAse (Sigma ...
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Harvest and Culture of Porcine Adipose-Derived Mesenchymal Stem Cells for Autologous TransplantationbioRxiv - Cell Biology 2020Quote: ... (Fat Processing Video) Phosphate-buffered saline (PBS; 100 mL) was mixed with 75 mg (±5 mg) of type A collagenase (Worthington Biochemical Corporation ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were chopped and incubated 5 min at 37°C in a HBSS-papain based lysis buffer (Worthington PAP) containing DNAse (0.01% ...
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bioRxiv - Developmental Biology 2019Quote: ... Ovaries were dissected into 1x PBS on ice and incubated in 5 mg/ml collagenase IV in PBS (Worthington Biochemicals) at RT for 45 min to dissociate the tissue ...
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bioRxiv - Physiology 2023Quote: ... Homogenate was digested with liver digestion buffer (5 ml HBSS w/o Ca2+/Mg2+, 200U/ml Collagenase IV (Worthington, USA) (37 °C ...
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bioRxiv - Physiology 2023Quote: ... Hearts were then perfused for 5 - 10 min with 0.125 mM CaCl2 KRH buffer supplemented with 1mg/mL of type II collagenase (Worthington Biochemical). Ventricles were cut into 0.1 mM CaCl2 solution ...
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bioRxiv - Immunology 2023Quote: ... The right lobes of the lungs were collected in 5 mL of digestion buffer (90% DMEM and 10% PBS and calcium with type 2 collagenase (180 U/ml) (Worthington) and DNase (15μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg mL-1 collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U mL-1 DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg/ml collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/ml DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... SNc regions from the slices were dissected out with a scalpel blade and digested with oxygenated high glucose HEPES-buffered saline containing papain (5–8 U/ml, Worthington, USA) for 20–30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... the dissociation buffer was warmed to 34□ and 5 mL was added to a vial of lyophilized papain (Worthington Biochemical Corporation). The solution was diluted 1:2 with additional dissociation buffer prior to sample dissociation.
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bioRxiv - Neuroscience 2020Quote: ... muscles were cut into 5 mm2 pieces with forceps and digested in 2 mg/mL collagenase II (Worthington Chemicals, Lakewood, NJ) for 1h at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells then were resuspended in 100 μl 1X PBS with CaCl2 (final concentration: 1mM) and TritonX100 (final concentration: 0.1%) and incubated with 5 Units micrococcal nuclease (Worthington Biochemical, LS004798) at 37°C for 3 min ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Genomics 2019Quote: Nuclei from 5×105 cells were aliquoted into microcentrifuge tubes and incubated at 37°C for 5 min with varying amounts of DNase (0 to 75 U; Worthington, LS006344). Digestion was stopped by addition of an equal volume of termination buffer [10 mM Tris (Sigma ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied...Cat# LS006344, 500 un, $90.00 Ask -
bioRxiv - Molecular Biology 2022Quote: ... Primary osteoblasts were isolated from the calvaria of 5-day-old mouse pups using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2020Quote: ... This was followed by a wash step and centrifugation at 500 x g for 5 min before removing the dissociation buffer and adding stock collagenase II (4.3 mg/ml) (Worthington, Lakewood, USA) and stock dispase (6.0 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... muscles were cut into 5 mm2 pieces with forceps and digested in 2 mg/mL collagenase II (Worthington Chemicals, Lakewood, NJ) for 1h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... The digested samples were washed with PBS and were further digested with 0.05% trypsin-EDTA for 5 minutes and 1 unit/ml neutral protease (dispase) (Worthington Biochemical, LS02109) plus 100 ug/ml Dnase (Roche ...
Product cited:
Animal Free, Partially purified. A lyophilized powder.Cat# LS02109, 1 gm, $171.00 Ask -
bioRxiv - Pharmacology and Toxicology 2024Quote: Primary calvarial osteoblasts were isolated from calvaria of 5-day-old neonates using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... The SNc regions were dissected out from each slice and incubated for 20–30 min at 36–37℃ in oxygenated high-glucose HEPES-buffered saline containing 4–8 U/ml papain (Worthington Biochemical Corp., USA, LK0003176). After enzymatic digestion ...
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bioRxiv - Neuroscience 2024Quote: ... ∼20 hCOs were chopped into small pieces using a #10 blade and treated with 30 U/ml Papain (Worthington Biochemical, CAS: 9001-73-4) for 60-90 minutes at 37 °C ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were minced into small pieces and transferred to a dounce-homogenizer containing 5 ml ice-cold HBSS with 20 µg/ml DNase I (Worthington, DPRF, LS006343). Tissue chunks were homogenized with 15 loose and 15 more tight strokes and the homogenate was transferred to a 50 ml falcon tube through a pre-wet 70 µm strainer ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. ...Cat# LS006343, 50 ku, $640.00 Ask -
bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and DNase I-treated for 30 min at 37°C (Worthington, 30U per 5 x 106 cells). After DNase I digestion ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were incubated statically at 37°C with 5% CO2 followed by plating on TSA plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and the desired antibiotic (250 µg/ml kanamycin or 200 µg/ml streptomycin) ...
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bioRxiv - Molecular Biology 2022Quote: Five 10um cryostat slices of patient polyps were digested for 5 min at 37°C with a titration of MNase: 20 units/mL and 200 unit/mL of MNase (Worthington Biochemical Corp.) in MNase cleavage buffer (4 mM MgCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... washed twice in HBSS and incubated during 30 min at 37°C in pre-warmed, oxygenated (95% O2, 5% CO2) Papain/DNase I (Worthington Biochemical Corporation) on a gyratory shaker (100 rpm) ...
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bioRxiv - Neuroscience 2024Quote: ... a total of ∼50 brains for each group was immediately transferred to an Eppendorf tube (1.5 mL) containing 200 µL AHS with Pronase (1 mg/mL; P5147, SigmaAldrich) and Dispase (1 mg/mL; LS02104, Worthington Biochemical Corporation). After tissue digestion for 30 min at 25°C ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Systems Biology 2019Quote: ... then the samples were diluted by ten folds with 100 mM ammonium bicarbonate buffer followed by digestion using lysyl endopeptidase (Lys-C; Wako Pure Chemical Corp., Osaka, Japan) for 4 hours and trypsin (Worthington Biochemical Corp., Lakewood, NJ, USA) for overnight at room temperature ...