Papain, Suspension
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full activity, the enzyme should be incubated in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS003124 |
| CAS # | 9001.73.4 |
| Pricing | 25 mg, $40.00 |
| Product Code | PAP |
| Activity/Specification | Activates to at least 20 units per mg protein |
| UNSPSC Code(s)* | 12352204.0 |
| Special Shipping/Handling | REQUIRES SPECIAL SHIPPING: ICE PACK |
| Source | Papaya Latex |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Neuroscience 2020Quote: ... digested with papain (#LS003124, Worthington, USA) for 30 min at 30° C and dissociated with micropipette tips in Neurobasal-A medium (#10888022 ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi were dissociated using 200 units Papain (Worthington LS003124, 200 units) for 40 minutes at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... cut in small pieces and incubated in PBS containing 5 mg/ml of papain (Worthington, LS003124), 0.24 mg/ml of L-cysteine ...
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bioRxiv - Developmental Biology 2024Quote: ... Whole striatal tissues including the globus pallidus were digested with 20 units/ml papain (LS003124, Worthington, Lakewood USA) diluted in dissection solution (5 mM MgCl2 and 5 mM HEPES in 1× Hanks’ balanced salt solution ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was dissociated using papain (LS003124, Worthington) dissolved in Hibernate A buffer (NC1787837 ...
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bioRxiv - Physiology 2024Quote: ... containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shaking water bath at 34°C for 30 min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9 mm ...
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bioRxiv - Molecular Biology 2022Quote: ... containing 2U/ml Papain (Worthington LS003124), 1U/ml Dispase II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated at 37°C with OPC papain solution (Worthington Cat#LS003124) for 20 minutes with constant inversion to prevent tissue aggregation ...