STEMxyme® 1, Collagenase/Neutral Protease (Dispase®), Animal Free
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and Animal Free Bacillus polymyxa neutral protease with a minimum of 250 CLS units and 1,000 caseinase units per mg dry weight. Designed for stem cell and other primary cell isolations and bioprocessing applications where introduction of potential animal derived pathogens must be prevented.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004106 |
| CAS # | 9001.12.1/42613.33.2 |
| Pricing | 50 mg, $110.00 |
| Product Code | STZ1 |
| Activity/Specification | ≥250 CLS/1,000 CAS units per milligram dry weight |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Clostridium histolyticum |
| Storage Conditions | Store at 2-8°C |
Citations for this product:
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bioRxiv - Neuroscience 2020Quote: Dissociated DRG cultures for calcium imaging were prepared from freshly excised DRGs according to our previously published protocol (see “Dissociated dorsal root ganglia cultures”, 11) with STEMzyme I (2mg/ml; Worthington, LS004106) replacing collagenase IV ...
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bioRxiv - Cell Biology 2019Quote: ... Niche cells from the bone marrow fraction were isolated by digestion with 1 mg/mL STEMxyme1 (Worthington, Ref#LS004106) and 1 mg/mL Dispase 1 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 0.4 ml Collagenase/Dispase (20mg/ml, cat.n.LS004106, Worthington)) for 1 h at 37°C with triturating every 15 min through a 1 ml tip cut to a ∼5 mm opening ...
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bioRxiv - Neuroscience 2024Quote: ... The DRG pieces were incubated in 5 ml HBSS containing 2 mg/mL Stemzyme (LS004106, Worthington Biochemical) and DNAse I (LS001239 ...
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bioRxiv - Neuroscience 2024Quote: ... a final enzyme solution consisting of 2mg/mL Stemzyme I (Worthington Biochemical, Cat#LS004106) and 0.1 mg/mL DNAse I was prepared in HBSS (Thermo Scientific ...
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bioRxiv - Physiology 2023Quote: ... The bone shaft was then cut into pieces and digested with 1 mg/mL STEMxyme1 (Worthington, Ref#LS004106) and 1 mg/mL Dispase II (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... a final enzyme solution consisting of 2mg/mL Stemzyme I (Worthington Biochemical, Cat#LS004106) and 0.1 mg/mL DNAse I was prepared in HBSS (Thermo Scientific ...