Collagenase, Type 4
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Suggested for pancreatic islet primary isolation. A dialyzed, lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004186 |
| CAS # | 9001.12.1 |
| Pricing | 100 mg, $42.00 |
| Product Code | CLS-4 |
| Activity/Specification | ≥160 units per mg dry weight |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Clostridium histolyticum |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Genomics 2020Quote: ... 100 µg/mL Collagenase IV (Worthington; catalog no. LS004186).
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bioRxiv - Immunology 2021Quote: ... Freshly isolated tumor tissues were minced into approximately 1 mm3 cubic pieces and digested using 0.1% collagenase IV (LS004186, Worthington), 0.002% DNAse I (D8071 ...
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bioRxiv - Bioengineering 2020Quote: ... Sample dissociation was performed in 500 µL of PBS + 25% FBS and 100 Units Collagenase Type IV (#LS004186, Worthington Biochemical). Samples were made piecemeal using scissors ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 0.25 mg/mL collagenase IV (Worthington #LS004186), 2% heat- inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: Skin tissued were finely minced with scissors and incubated in a digestion cocktail containing collagenase IV (3.2 mg.ml−1, Worthington, LS004186) and DNAse (20 μg ml−1 ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... The hearts from all embryos were then sorted into two tubes based on fluoresce signal and digested with 300μl 500 U/ml collagenase IV (Worthington #LS004186), 1.2 U/ml dispase (Worthington #LS02100) ...
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bioRxiv - Immunology 2019Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... and Collagenase IV (Worthington, LS004186) and 20ug/mL DNAse (Worthington ...
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bioRxiv - Developmental Biology 2020Quote: ... 500 U/ml collagenase IV (Worthington #LS004186), 32 U/ml DNase I (Worthington #LS002007) ...