Labshake search
Citations for Worthington Biochemical :
101 - 142 of 142 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... was dissected on ice and minced using two razor blades in a few drops of pre-warmed (37°C) Hibernate A medium containing 20 units/mL papain (Worthington LK003178). Minced tissue was incubated in 3.125 mL pre- warmed papain solution for 10 minutes at 37°C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... Spinal cords were minced on ice with a razor blade then digested for 15 minutes on a shaker at 37°C in 5mL papain solution (300U papain solution (Worthington, LS003126) was diluted in HBSS supplemented with 1:100 DNAse and the transcription/translation inhibitor cocktail) ...
-
ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... and hearts were perfused retrogradely for 15-18 min at 37°C with HEPES-buffer containing 100 mg/mL collagenase type 2 (Cat#9001-12-1, Worthington, USA) and digested ...
-
bioRxiv - Neuroscience 2024Quote: Cortical assembloids were washed with 1X PBS and dissociated at 37°C 5% CO2 on an orbital shaker with Papain (Worthington #LK003150) for 30 minutes ...
-
bioRxiv - Bioengineering 2024Quote: Cardiomyocytes were dissociated by Langendorff heart perfusion of a 37°C digestion perfusion solution containing 300 U/mL collagenase (Worthington, LS004176). Collagenase digested tissue was minced and dissociated cardiomyocytes filtered through a 100 µm mesh filter (Falcon ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and both fragments were separately digested in a petri dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) for 12 hours ...
-
bioRxiv - Developmental Biology 2024Quote: ... minced vessels were incubated for 60 minutes at 37°C with agitation in an enzymatic cocktail consisting of 1 mg/ml collagenase I (Worthington, LS004196), 1 mg/ml Dispase II (Gibco ...
-
bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
-
bioRxiv - Neuroscience 2020Quote: Brain samples were minced with scissors and then incubated at 37 °C on a roller for 30 min in digest buffer containing 1.25 mg/mL Collagenase Type 4 (Worthington, Lakewood, NJ, USA) and 100 U/mL DNAseI (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... The hippocampal neurons were then dissociated via incubation at 37°C for 30 minutes in 2% papain solution (Worthington; catalog no. LS003127). Following incubation ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003127, 1 gm, $595.00 Ask -
bioRxiv - Genomics 2020Quote: ... Chromatin digestion was carried out at 30°C for 12 minutes with different concentrations of micrococcal nuclease (MNase, Worthington Biochemicals; cat# LS004798). The digestion was stopped by adding EDTA to final concentration of 10 mM ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Systems Biology 2022Quote: ... testes were placed in a 15 ml conical tube containing 2 ml of prewarmed (35 °C) collagenase solution (1 mg/ml type I collagenase (Worthington, cat. # LS004196) and 60 U/ml Turbo Nuclease (Nacalai ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2021Quote: ... Cells were infected with lung homogenate supernatants at varying dilutions for 45 min at 37°C before the addition of Leibovitz L15 or MEM medium containing 0.9% agarose overlay containing Trypsin (Worthington Biochemical, NJ, USA), as previously described[54] ...
-
bioRxiv - Neuroscience 2022Quote: ... which were immediately chopped and digested at 37°C with rotation in a solution containing 2mg/ml papain (Worthington Biochemical Corporation, LS003126) and 50 μg/ml DNase I in PBS ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Genomics 2023Quote: ... Tissue was coarsely chopped with a sterile scalpel and digested for 1hr at 34°C in a pH-equilibrated papain solution (Worthington, Lakewood, NJ) with constant CO2 flow over the enzyme solution ...
-
bioRxiv - Physiology 2024Quote: ... CD1 wild type) were harvested and enzymatically digested for 45 min at 37 °C with collagenase type II (520 U/ml, Worthington, NJ US) (20) ...
-
bioRxiv - Neuroscience 2023Quote: ... washed twice in HBSS and incubated during 30 min at 37°C in pre-warmed, oxygenated (95% O2, 5% CO2) Papain/DNase I (Worthington Biochemical Corporation) on a gyratory shaker (100 rpm) ...
-
bioRxiv - Immunology 2023Quote: ... for 5 min at 4°C to remove circulating cells and then digested in RPMI 1640 with 1 mg/mL Type 2 collagenase (Worthington Biochemical LS004177) for 45 min at 37°C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Retinas were detached from the retinal pigmented epithelium and incubated for 15 min at 37°C in a solution containing papain (10 U/ml, Worthington; Lakewood, NJ) and L-Cysteine hydrochloride (∼1 mg/mL ...
-
bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and DNase I-treated for 30 min at 37°C (Worthington, 30U per 5 x 106 cells). After DNase I digestion ...
-
bioRxiv - Molecular Biology 2022Quote: Five 10um cryostat slices of patient polyps were digested for 5 min at 37°C with a titration of MNase: 20 units/mL and 200 unit/mL of MNase (Worthington Biochemical Corp.) in MNase cleavage buffer (4 mM MgCl2 ...
-
bioRxiv - Bioengineering 2024Quote: ... The DRGs from 4 pups were digested for 30 min at 37°C using 13.33 U/ml papain (Worthington, cat. No. LS003126). The DRGs were then centrifuged for 1 min at 500g ...
-
bioRxiv - Cell Biology 2024Quote: ... Two to three pieces of 0.5g testis tissue was placed in a 50mL Falcon tube with 10mL prewarmed collagenase solution at 32 °C (1 mg/mL collagenase I (Worthington Biochemicals #LS004196), 1 mM EDTA ...
-
bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2022Quote: Cells were isolated from semitendinosus muscles of 10 Belgian Blue cattle (Supplementary Table 1) by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
-
bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg mL-1 collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U mL-1 DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... Brain tissues were manually minced using razor blades and enzymatically dissociated at 37°C for 30 min with 1 mg/mL collagenase type IV (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/mL DNase 1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg/ml collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/ml DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2023Quote: ... and then digested for 30 minutes under gentle agitation at 37°C with 7 mL of 4 mg/mL of type III collagenase (Worthington Biochemical, Lakewood, NJ) and 0.05 mg/mL of DNase I (Sigma ...
-
Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Genomics 2024Quote: ... Neurons so produced were then detached from the plate for CHi-C and Cut and Run/RNA-Seq assays using a mix of Accutase and Papain (Worthington Co, catalog #LS003126). Two independent differentiations were conducted and used as biological replicates for the CHi-C assay.
-
bioRxiv - Cancer Biology 2024Quote: ... the tumors were minced and then incubated for 30 minutes at 37 °C with 200U of Collagenase III (Worthington, Lakewood, New Jersey, USA). Both tumors and draining lymph nodes were then filtered through 70µm nylon filters ...
-
bioRxiv - Cell Biology 2024Quote: ... Each leaflet was incubated for 20 min at 37 °C in 2 mg/mL type II collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA) in cell culture medium (advanced Dulbecco’s modified Eagle’s medium (Ad DMEM ...
-
bioRxiv - Neuroscience 2021Quote: ... animals were killed by decapitation and thoracolumbar DRG were collected on cold HBSS and enzymatically digested at 37°C for 40min with collagenase II (Biochrom, 2mg/ml, 235U/mg) for mice and collagenase I (Worthington, 4mg/ml, 370U/mg) for rats and dispase II (Gibco ...
-
bioRxiv - Physiology 2020Quote: ... Tissue chunks were transferred into a digestion device and then subjected to sequential (6 steps) enzymatic digestion (at 37°C) using collagenase type V (Worthington Biochemical Corporation, Lakewood, NJ) 250 U/mL and protease type XXIV (Sigma-Aldrich Co. ...
-
bioRxiv - Neuroscience 2023Quote: ... and digested at 37°C for 20 min with Collagenases-I and - IV (10 U/mL, 400 U/mL final, Worthington, Cat# LS004194, and LS004184) and DNase I (30 U/mL ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Plant Biology 2024Quote: ... and incubated at 37 °C for 1 h followed by the addition of 1.5 mg/mL trypsin inhibitor (Worthington Biochemical Corp, Lakewood, NJ USA) to inactivate trypsin ...
-
bioRxiv - Microbiology 2020Quote: ... Approximately 10~15 mg of proteins were then used for a sequential digestion using lysyl endopeptidase (Lys-C; Wako Pure Chemical Corp., Osaka, Japan) and trypsin (Worthington Biochemical Corp., Lakewood, NJ, USA). Briefly ...
-
bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...