Nuclease, Micrococcal
Chromatographically purified to be essentially homogeneous chromatographically and electrophoretically (SDS-PAGE). A lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004798 |
| CAS # | 9013.53.0 |
| Pricing | 45 ku, $211.00 |
| Product Code | NFCP |
| Activity/Specification | ≥6,000 units per mg protein |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Staphylococcus aureus (Strain Foggi) |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Genomics 2022Quote: ... 100 ul NP buffer supplemented with 40 units of Micrococcal Nuclease (Worthington MNase, LS004798) were added and samples were incubated for 20 minutes at 37°C ...
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bioRxiv - Genomics 2020Quote: ... then digested with a concentration gradient of MNase (Worthington Biochemical Corporation #LS004798)for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... High digests (80% mononucleosomes) required 50U of micrococcal nuclease (Worthington, LS004798) and for the low digests ...
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bioRxiv - Genomics 2020Quote: ... Chromatin was fragmented into ∼90% mononucleosome and ∼10% dinucleosome with Micrococcal Nuclease (MNase) (Worthington Biochem #LS004798). We recommend titrating the MNase digesting condition for each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM β-mercaptoethanol and 0.075% NP-40) before being exposed to MNase (Worthington, LS004798) digestion titration to generate the appropriate size of chromatin fragments as follow ...
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bioRxiv - Genomics 2020Quote: ... Chromatin digestion was carried out at 30°C for 12 minutes with different concentrations of micrococcal nuclease (MNase, Worthington Biochemicals; cat# LS004798). The digestion was stopped by adding EDTA to final concentration of 10 mM ...
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bioRxiv - Genetics 2020Quote: ... followed by addition of 4 U MNase (Worthington Biochemical LS004798; Lakewood, NJ) per 1 × 107 cells ...
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bioRxiv - Genomics 2022Quote: ... and pellet was re-suspended in 10ul of lysis buffer supplemented with 17.5 units of micrococcal nuclease (Worthington MNase, LS004798) and chilled on ice for 10 minutes ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... The nuclei suspension was preincubated at 37°C for 5 min and followed by micrococcal nuclease (Worthington, cat# LS004798) digestion was carried out by following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Added 2.5 U MNase (Worthington Biochem #LS004798) to the solution and incubated at 37°C for 10 min ...