PDS Kit, Papain Vial
A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1 mM L-cysteine with 0.5 mM EDTA.
Supplier | Worthington Biochemical |
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Product # | LK003178 |
CAS # | 9001.73.4 |
Pricing | 5 vi, $98.00 |
Product Code | PAP2 |
Activity/Specification | ≥100 units per vial |
UNSPSC Code(s)* | 12352204.0 |
Source | Papaya Latex |
Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Neuroscience 2020Quote: ... Dissected striatal tissue was incubated with papain (LK003178, Worthington Biochemical Corporation, Lakewood, NJ) for 25 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Neural stem cells were isolated by enzymatic digestion using papain dissociation (Worthington, LK003178). Cells were seeded in NSC media (DMEM/F12 supplemented with N2 (1x) ...
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bioRxiv - Neuroscience 2021Quote: ... DM+Papain/L-Cysteine (3.4 units Papain and 0.172 mM L-Cysteine; Worthington Biochemical, LK003178) for 3-5 min at 37° C ...
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bioRxiv - Neuroscience 2020Quote: ... transfer either only the cerebellum or the cerebellum including pones to a 15 mL tube containing 20 U/mL papain (Worthington, #LK003178) solved in preparation solution and warmed up to 36 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The cut sections are dissociated while partially frozen in 300 μL of 20 units papain (# LK003178, Worthington Biochemical Corporation) in 15 mL Hibernate-E media (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then dissociated with papain (Worthington, Cat# LK003178) supplemented with DNase (Worthington ...
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bioRxiv - Neuroscience 2020Quote: ... Dissected cortical or hippocampal tissue was incubated with papain (Worthington LK003178) for 25 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... papain (PDS Kit; LK003178; Worthington Biochemical Corporation, Lakewood, NJ), DNase I (90083 ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were rinsed in cold RNase-free PBS and transferred to freshly made dissociation buffer (300 μL of 100 U/mL heat-activated papain (Worthington Biochemical Corporation #LK003178) added with 6 μL of 2.5 mg/mL liberase™ (Sigma-Aldrich #5401119001)) ...
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bioRxiv - Neuroscience 2021Quote: ... immature neurons were dissociated using accutase (Worthington LK003178) and replated at a density of 3*106 cells/well in Matrigel-coated 6-well plates containing NB media supplemented with BDNF (Thermo Fisher PHC7074) ...