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Citations for Promega :
7801 - 7850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Genomic DNA was extracted using the Wizard SV genomic DNA purification kit (Promega). Multiplicity of infection (MOI ...
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bioRxiv - Genomics 2023Quote: ... DNA and cDNA concentrations were determined using a Quantifluor® Assay (Promega®). Samples were diluted to 200 pg/μL or 100 pg/μL for Nextera XT (Illumina ...
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bioRxiv - Genomics 2023Quote: ... The locus was amplified with GoTaq polymerase (Promega #PRM7123) using the PCR program ...
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bioRxiv - Genomics 2023Quote: ... TRs were amplified using the forward and reverse primers plus an M13 primer with one of the four fluorophores with GoTaq polymerase (Promega #PRM7123) using PCR program ...
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bioRxiv - Genomics 2023Quote: ... Cell viability was measured using a Cell Titer-Glo® Luminescent Cell Viability Assay kit (Promega) according to the manufacturer protocol ...
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bioRxiv - Genomics 2023Quote: ... with a higher binding capacity and cleaner eluate than traditional silica-based DNA purification systems (Sui, et al., 2020; Moeller, et al., 2014; Dunbar, et al., 2018; Promega, 2020). The platform also permits a high-throughput automated genomic DNA isolation from 48 samples in 40 minutes (Promega ...
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bioRxiv - Genomics 2023Quote: ... The platform also permits a high-throughput automated genomic DNA isolation from 48 samples in 40 minutes (Promega, 2020) making this system compatible with larger sample sets.
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bioRxiv - Genomics 2023Quote: ... Histones were then digested with 1 µg of sequencing grade trypsin (Promega) diluted in 50 mM ammonium bicarbonate (1:20 ...
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bioRxiv - Genomics 2023Quote: ... Each culture was then sampled by mixing 80uL of culture with 80uL of DI water and 80uL of Nano-Glo Luciferase Assay System reagent according to the manufacturer’s instructions (Promega, Madison, WI). Luminescence signals were measured using a BioTek Synergy H1 microplate reader and then normalized using OD600 measurements as a proxy for cell density.
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA of each of these samples was extracted with the Wizard Genomic DNA Purification Kit (Promega) following manufacturer recommendation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... individual specimens were mounted in either 80% Glycerol (Promega H5433) 20% 5X SSCT or 2% methyl cellulose in 5X SSCT (for posterior imaging ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were digested overnight at 37 °C using MS-grade trypsin/Lys-C Mix (Promega, Madison, WI) (enzyme/substrate of 1:50 w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing-grade trypsin was from Promega (Madison, WI, USA). Trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2023Quote: ... digested using 20 ng/µL sequencing-grade trypsin (Promega) for 1 hr at 47 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μL injections of Furimazine (Nano-Glo® Luciferase Assay Substrate, Promega) were applied ...
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bioRxiv - Cell Biology 2023Quote: ... and dehydrated for trypsin digestion (Promega, V5113). Obtained peptide mix was desalted with a MicroSpin C18 column (The Nest Group ...
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bioRxiv - Cell Biology 2023Quote: DNA was prepared from bacterial cultures grown at 37°C using a Midiprep kit (PureYield Plasmid Midiprep System, Promega) according to the manufacturer’s instructions ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... supplemented with 1 mM CaCl2 and digested with 2 μg trypsin (Promega) for 15 h at 37ᵒC ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... 1 mM CaCl2 with 1 µg trypsin (Promega). Digested proteins were acidified with TFA to pH < 2 and RapiGest SF was precipitated out ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-p-JNK (pTPpY, Promega, USA-1:100 dilution); 8 ...
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bioRxiv - Cell Biology 2023Quote: ... 100µl of CellTiter-Glo reagent (Promega, cat. no. G7572) was added to the wells while samples thawed at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... and finally radiolabelled precursor proteins were synthesized with the Flexi rabbit reticulocyte lysate system (Promega). 35S-radiolabeled precursor proteins were incubated with isolated mitochondria in import buffer (3% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... followed by addition of 6 μL of FuGene HD (Promega). This entire mix was dripped onto the culture and incubated without shaking ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with packaging vectors pMD2.G and psPAX2 along with relevant shRNA using Fugene 6 transfection reagent (Promega). Viral particles were harvested after 48 hours in DEMEM/ 40% FBS ...
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bioRxiv - Immunology 2023Quote: ... and an equal volume of Bio-Glo™ Luciferase reagent (G7940; Promega, Madison, Wisconsin, USA) was added to each well and rocked gently at RT for 15 mins ...
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bioRxiv - Immunology 2023Quote: ... cells that had migrated into the bottom chamber were quantified using Cell Titer Glo (Promega Corporation) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The purification of total RNA and reverse transcription to synthesize cDNA were performed using a ReliaPrep RNA Cell Miniprep System (Promega) and ReverTra Ace qPCR RT Master Mix (TOYOBO ...
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bioRxiv - Immunology 2023Quote: FACS-purified T cell subsets were incubated overnight with a TGF-β reporter cell line28 and luciferase activity detected via the Luciferase Assay System (Promega). TGF-β activity was determined as previously described16.
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bioRxiv - Immunology 2023Quote: BacTiter-Glo Microbial Cell Viability Kit (Promega) was used to assess bacterial cell viability and metabolism by measuring luminescence correlated with amount of intracellular ATP ...
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The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... and pMD2.G (1 μg) using Fugene HD transfection reagent (Promega) into HEK 293T cells ...
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The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... the indicated plasmids were mixed with a control RFP vector to a total of 1.0 μg DNA in 65 μL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... and 22 days post-surgery on a Lago X (Spectral Instruments Imaging) after isoflurane anesthesia and intraperitoneal administration of 150 µg D-luciferin (Promega, E1605) per gram body weight to monitor bioluminescence ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The sequences of SmBiT and LgBiT were kindly provided by Promega. Spacers were added to the C terminus of LgBiT or SmBiT to yield different distances between split luciferases and cell membranes ...
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bioRxiv - Cell Biology 2023Quote: ... obtained by amplification in DH10BacY cells were used to transfect Sf9 insect cells using FuGene transfection reagent (Promega). About 7 days after transfection the V0 virus was harvested and used to produce a V1 virus stock ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was used for first-strand cDNA synthesis with the Reverse Transcription System I (Promega, Madison, WI, USA), then diluted 1:50 in water ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg protein was used for dual luciferase transactivation assay using Dual-Luciferase® Reporter Assay System (Promega) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... wings were transferred to alkaline-phosphatase buffer (see supp Table S4 for composition) supplemented with NBT-BCIP (Promega, Cat No. S3771) and incubated in the dark till the development of color ...
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bioRxiv - Biochemistry 2023Quote: ... and the HaloTag pHTN HaloTag CMV-neo vector purchased from Promega (Madison, WI, USA). Restriction enzymes Not1-EcoR1 ...
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bioRxiv - Biochemistry 2023Quote: ... Twenty-five µl of 100x dilution of NanoBRET NanoGlo substrate (Promega, Madison, MA, USA) was added per well ...
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bioRxiv - Genomics 2023Quote: ... and Quantus Fluorometer (Promega, USA) using a DNA QuantiFluor ONE dsDNA System (Promega ...
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bioRxiv - Genomics 2023Quote: ... using a DNA QuantiFluor ONE dsDNA System (Promega, USA). Only pure DNA (A260/A280 ∼1.8 and A260/A230 ∼2.0 according to NanoDrop ...
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bioRxiv - Microbiology 2023Quote: ... pRL-SV40 vector (Promega, Germany) encoding Renilla luciferase was used as a template DNA ...
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bioRxiv - Microbiology 2023Quote: The effect of pyocin SX2 on protein synthesis was assessed by an in vitro transcription-translation assay (E. coli T7 S30 Extract System for Circular DNA. Promega, Germany). pRL-SV40 vector (Promega ...
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bioRxiv - Microbiology 2023Quote: ... The quantity of the reporter protein was examined by measuring its enzymatic activity through the Renilla Luciferase Assay System (Promega, Germany), as per manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: The GTP levels were measured using a GTPase-Glo Assay kit (Promega) which employs an ATP-linked luciferase reaction ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with EnduRen substrate (Promega, 1:1000) and incubated at 37 °C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Strap binding buffer was applied to precipitate proteins on quartz and proteolysis took place during 14 hrs at 37°C with 1 µg Trypsin sequencing grade (Promega). After speed-vacuum drying of eluted peptides ...
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bioRxiv - Molecular Biology 2023Quote: ... alkylated with iodoacetamide and in-gel digested with 0.1 µg trypsin (V511, Promega) per slice using a Freedom EVO150 robotic platform (Tecan Traging AG ...
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bioRxiv - Molecular Biology 2023Quote: ... The milk solution was removed and the membrane was incubated with a 1:1,000 dilution of streptavidin-alkaline phosphatase antibody (Promega) in TBS-T for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The MTS assay was carried out using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega) according to the manufacturer’s instructions ...