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Citations for Promega :
8051 - 8100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... BRD9-iBIT KI pool (PROMEGA #CS3023119 BRD9-HiBiT KI HEK293 cells (donor sex ...
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bioRxiv - Developmental Biology 2023Quote: ... Reporter Lysis Buffer (Promega, #E1531) was employed.
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bioRxiv - Developmental Biology 2023Quote: ... we initially transfected HEK293T cells using the Fugene HD Transfection Reagent (Promega, E2311) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... and Protease Inhibitor Cocktail (Promega Cat. No. G6521). The following antibodies were used ...
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bioRxiv - Biochemistry 2023Quote: ... Transfections of the PX459 constructs were performed individually using FuGENE 6 Transfection Reagent (Promega E2691) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNAs of bacteria were extracted using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA).
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bioRxiv - Bioengineering 2023Quote: ... 10 µl supernatant was incubated with 10 µl of freshly prepared nucleotide detection reagent for UDP-Glo assays (Promega, Cat#: V6961) according to manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2023Quote: ... and reverse transcribed using the Reverse Transcription System (Promega). Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... Furimazine (Promega) was added to each well in accordance with the manufacturer’s guidelines and readings taken at 120 second intervals for up to 40 minutes (PHERAstar FS microplate reader ...
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bioRxiv - Cell Biology 2023Quote: ... and then they were transfected with the expression plasmid DNAs by using FuGENE 6 (Promega, Madison, WI).
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bioRxiv - Biophysics 2023Quote: To determine the GTPase activity of GBP1 the GTPase-Glo™ Assay (Promega) was utilised (50) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a second DNase treatment was performed with RQ1 RNase-free DNase (Promega, Charbonnières-les-Bains, France), followed by RNA clean-up using the RNeasy Plant Mini Kit ...
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bioRxiv - Genetics 2023Quote: ... and used for thermal cycling (35× 30sec at 95°C, 55°C and 72°C) with 1X GoTaq G2 Master Mixes (Promega, cat.no.M7823), 0.2µM CTNS and 0.2µM bactin1 primers (Supplementary Table S2) ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK cells were transfected with both heavy and light chain BCR constructs using FuGENE HD transfection reagent (Promega) in a 1:3 ratio ...
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bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.12 mM EDTA and 100 µg/mL RNAse A (Promega A797c, Promega) and incubated 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.12 mM EDTA and 100 µg/mL RNAse A (Promega A797c, Promega) and incubated 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Individual gel piece was subjected to in-gel digestion using trypsin (Promega). The resulting dried peptides were analyzed in technical duplicate on a ThermoFisher Orbitrap Fusion Lumos Tribrid mass spectrometry system equipped with an EASY-nLC 1200 ultrahigh-pressure liquid chromatography system interfaced via a Nanospray Flex nanoelectrospray source ...
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bioRxiv - Neuroscience 2023Quote: ... After embedding in 4% low-melting point agarose (Promega, #V2111) in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... Luciferase activity was then measured at room temperature (below 25 °C) using the Luciferase Assay System (Promega Corporation) in a Glomax luminometer (Promega Corporation ...
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bioRxiv - Neuroscience 2023Quote: A Glutamine/Glutamate-Glo™ Assay (Promega) was used to measure intracellular concentrations of glutamine and glutamate ...
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bioRxiv - Neuroscience 2023Quote: ... After sample preparation the assay was performed as per the protocol provided by Promega.
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bioRxiv - Plant Biology 2023Quote: ... in a Glomax luminometer (Promega Corporation) with a 10-s measurement read for luciferase activity.
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bioRxiv - Plant Biology 2023Quote: ... and the total protein contents were quantified using BCA Protein Assay Reagent (Promega Corporation). The supernatants were stored at –80 ℃ until subsequent analysis.
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bioRxiv - Plant Biology 2023Quote: The total proteins (250 μg for each sample) were digested by FASP procedure using trypsin (Promega Corporation) as described previously60 ...
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bioRxiv - Plant Biology 2023Quote: ... we used in vitro translation system based on Wheat Germ Extract (Promega Corporation), which has been used for SCR validation as reported previously31 ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 μg of the linearized DNA was transcribed in vitro using T7 RNA polymerase (Promega Corporation). The obtained RNA was then purified using GeneJET kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Luciferase refolding assay: QuantiLum Recombinant Luciferase (Promega) was diluted to 55 µM in denaturation buffer containing 6M Guanidium/HCl and 1 mM DTT during 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... In the first round of selection 400 nM of Xl-HAS-1-loaded nanodiscs diluted in the selection buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% BSA) was immobilized on streptavidin paramagnetic beads (Promega). Beads were washed three times in the selection buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were resuspended in 500 µL of 100 mM Tris-HCl pH 8.0 containing 2 mM CaCl2 and digested at 37 °C for 1 h with 0.2 μg of trypsin/LysC (#V5073 Promega). The samples were then loaded onto homemade SepPak C18 Tips packed by stacking three AttractSPE disk (#SPE-Disks-Bio-C18–100.47.20 ...
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bioRxiv - Immunology 2023Quote: ... and the cells were lysed by the addition of 30 µl of passive lysis buffer (Promega) and three freeze-thaw cycles ...
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bioRxiv - Microbiology 2023Quote: ... and PCR amplified using the GoTaq qPCR system (Promega A6002) on a LightCycler 480 (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... 80 mM ammonium bicarbonate solution and digested with modified trypsin (Promega) by overnight incubation at a 1:50 enzyme-to-substrate ratio at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cloned gene fragments were inserted into the pGEM-T Easy vector (Promega, A1360) and transformed into One Shot Top 10 chemically competent E ...
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Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopybioRxiv - Bioengineering 2023Quote: ... The monoclonal cells with successful EGFP incorporation were identified by PCR screening using GoTaq Polymerase (Promega). The clonal SUM159 cells expressing EGFP-Rab11A+/+ were subjected to the second round of genome editing to incorporate Lamp1-Halo in the genome as described above ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μL random primers (50 ng/μL, Promega, C1181), 2 μL 0.1 M DTT ...
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bioRxiv - Bioengineering 2023Quote: ... cell viability was evaluated using the CellTiter-Glo 2.0 Assay (Promega) dispensed by the Integra Viaflo Assist Pipetting Platform (Integra Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... and cells were lysed by overnight incubation with Renilla luciferase assay lysis buffer (Promega) at −80°C ...
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bioRxiv - Cell Biology 2023Quote: ... using HC T4 Ligase and rapid ligation buffer (Promega, Southampton, UK). Constructs encoding BirA*-RAB18 ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µL of CellTiter-Glo (Catalog No. G9242, Promega) was added into each well (containing 100 µL of culture media ...
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bioRxiv - Developmental Biology 2023Quote: ... using Fugene transfection reagent (Promega) in DMEM containing 10% FBS and Pen/Strep ...
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bioRxiv - Genomics 2023Quote: ... 20 ml PCR reactions contained 10 ml GoTaq® green PCR Master mix (Promega), 6 ml nuclease-free water ...
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bioRxiv - Immunology 2023Quote: ... or BioGlo (Promega) in PBS with Ca2+ and Mg2+ was added to cells ...
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bioRxiv - Immunology 2023Quote: ... ONE-Glo EX (Promega) was added to each well and the cells were incubated for 5 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability of each sample in triplicates at various time points was measured using a luminescence assay of ATP (CellTiter-Glo 2.0 Assay, Promega, Cat. #G9242) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured after 72hrs using CellTiter-Glo (Promega). EC50 was calculated using GraphPad PRISM 8 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified using GoScript™ Reverse Transcription System (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Proteasome activity was estimated using Proteasome Glo® Chemotrypsin-like kit (Promega) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatin was then precleared with MagneGST magnetic beads (Promega) for 1 h at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... and 0.4% (v/v) RNasin (Promega,