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Citations for Promega :
7851 - 7900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega). Analyses were carried out in the QuantStudio™ 5 Real-Time PCR System ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA synthesis was performed using ImProm-II™ Reverse Transcriptional System (A3800 - Promega) with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... along with the NBT and BCIP (Promega) detection system.
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bioRxiv - Cell Biology 2023Quote: ... cloned in pGEM-T vector (Promega) and sequenced (Macrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA extraction was performed according to the manufacturer’s instructions and cDNA was obtained by means of MMLV reverse transcriptase (Promega) using oligo dT with the protocol provided with the enzyme ...
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bioRxiv - Cell Biology 2023Quote: ... using HC T4 Ligase and rapid ligation buffer (Promega). Sequences of all recombinant plasmids were verified by direct sequencing ...
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bioRxiv - Cell Biology 2023Quote: Heat shock-induced cytotoxicity was determined using the LDH-Glo cytotoxicity assay (Promega, catalog number: J2380) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generation of the 18S and sgE RNA standards have been outlined previously.31 Both PCR products were serially diluted to produce standard curves in the range of 5 x 108 - 5 copies/reaction via a 10-fold serial dilution ...
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bioRxiv - Microbiology 2023Quote: ... GoTaq® Probe 1-Step RT-qPCR System was purchased from Promega (US). SARS-CoV-2 (2019nCoV ...
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bioRxiv - Microbiology 2023Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences,30 and were used at 300 nM and 200 nM ...
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bioRxiv - Microbiology 2023Quote: The viral RNA derived from the lung and nasal turbinate samples was quantified using a protocol for quantifying the SARS-CoV-2 sub-genomic E gene RNA (sgE)29 using the GoTaq® Probe 1-Step RT-qPCR System (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... Intracellular levels of NAD/NADH were measured using the NAD/NADH-GloTM quantitation kit ((Promega, G9071) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... live-cell renilla luciferase (Rluc) substrate (EnduRen, Promega) was added ...
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bioRxiv - Biophysics 2023Quote: ... or purified LgBiT-substrate cocktail (Promega). After incubation with appropriate HRP-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher) ...
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bioRxiv - Biophysics 2023Quote: ... equal volumes of HiBiT-N VLPs and hACE2-LgBiT EVs were mixed with nanoluc substrate (cat#N2420, Promega) and trypsin (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The pCMV-LgBiT expression plasmid was purchased from Promega. pcDNA3.1-hACE2-C9 was obtained from Dr ...
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bioRxiv - Developmental Biology 2023Quote: ... Concentration and fragment size of amplified cDNA was measured with QuantiFluor® dsDNA (Promega) and Fragment Analyzer (Advanced Analytical ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL of 50% Cell-Titer Glo (Promega cat G7573) diluted in water was added to the cells and luminescence measured on an EnVision Plate Reader (PerkinElmer).
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bioRxiv - Cell Biology 2023Quote: ... cytochrome P450 3A4 activity (CYP3A4) was measured using a luciferin-IPA kit (Promega cat V9001) following the lytic protocol with 3 μM IPA ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... followed by overnight modified trypsin digestion (Promega) at a ratio of 1/100 (enzyme/proteins ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with or without 50 μM adenosine 5′-triphosphate (ATP) at 30 °C for 30 minutes followed by kinase assays using ADP-Glo Kinase Assay kit (Promega). Data were analyzed using GraphPad Prism 9 ...
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bioRxiv - Cell Biology 2023Quote: ... were kindly provided by Promega. N-terminal NLuc orientations were used for CDKL5 ...
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bioRxiv - Immunology 2023Quote: ... 30 μl of 0.02 μg/μl of trypsin (Promega V5111) solution was added to the gel pieces for 20 min followed by addition of 60 μl solution containing 40 mM NH4HCO3 / 10% ACN to completely cover the gel pieces and the samples were incubated at 37°C for 18 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Luminescence was measured in 96-well format using Nano-Glo Luciferase (Promega) and a Modulus luminescence plate reader.
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bioRxiv - Developmental Biology 2023Quote: ... gBlocks were inserted using sequence-and ligase-independent cloning (58) upstream of the coding sequence for a secreted nano-luciferase following digestion of vector pNL2.3 (Promega) using EcoRV and HindIII ...
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bioRxiv - Neuroscience 2023Quote: ... exposed to ProteasomeGlo® (Promega, G8660) and incubated for 30 minutes at RT ...
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bioRxiv - Biophysics 2023Quote: ... and the suspension was applied on Flex-Column (420400-2510) connected to Vac-Man vacuum manifold (Promega).
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bioRxiv - Bioengineering 2023Quote: ... cells were stained with JF549 conjugated Halotag ligands (Promega) at 200nM concentration in 2% FBS/PBS for 20 min at RT ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then digested first with 6.6μg of LysC at room temperature overnight followed with 1.65ug sequencing grade trypsin (Promega) for 6 hours at 47°C ...
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bioRxiv - Bioengineering 2023Quote: Cell viability was determined using the CellTiter-Glo Luminescent Cell Viability Assay Kit (Catalog No. G7570, Promega) for each sample according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was isolated using a PureYield Plasmid Midi Preparation Kit (Promega, USA) and clones verified via sequencing ...
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bioRxiv - Bioengineering 2023Quote: ... Caspase-3/7 activity was measured 96 h after treatment using Caspase 3/7-Glo (Promega) according to the manufacturer’s directions ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained with Janelia Fluor (JF) 549 conjugated Halotag ligands (Promega) at 200 nM concentration in 2% FBS/PBS at 37 °C for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 20 μl of passive lysis buffer (Promega) and lysis allow to happen for 15 minutes at room temperature with shaking at 40 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... Luciferase activity was assayed using Dual-Luciferase Reporter Assay Kit (Promega) in 96-well black plates ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were further diluted with 50 mM Tris pH 7.9 pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 µg trypsin (Promega) (1/100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with CellTiter-Glo Reagent according to the manufacturer’s protocol (Promega, #G924C) and incubated 15 minutes under shaking ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified using GoScript™ Reverse Transcription System (Promega) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... with the exception of using RNasin 40 U/mL (Promega, Madison, WI) as the RNase inhibitor ...
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bioRxiv - Bioengineering 2023Quote: The 36PillarPlate with HLOs was sandwiched with an opaque white 384-well plate containing 20 μL of CellTiter-Glo® Reagent from CellTiter-Glo® cell viability kit (Promega, Madison, WI, USA) and 20 μL of cell culture medium in each well to measure cellular adenosine triphosphate (ATP ...
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bioRxiv - Bioengineering 2023Quote: ... FuGENE HD (E2311, Promega); Lipofectamine 3000 (L3000001 ...
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bioRxiv - Bioengineering 2023Quote: ... HiBiT detection was performed using the Nano-Glo HiBiT Lytic Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... an extended time-released substrate (Promega), and plates were incubated at 37 °C ...
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Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopybioRxiv - Bioengineering 2023Quote: ... The transfected cells were stained by the Janelia Fluor 646 HaloTag Ligands (Promega) and then enriched by FACS ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Samples were subsequently digested with trypsin (Promega Cat#:V511A) overnight using 1:20 (w/w ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... deep 96 well plate (V6771, Promega) in darkness in a 28°C incubator for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μg of the protein lysates were diluted to 50 μL using the M-PER buffer followed by complementation with LgBiT using Nano-Glo HiBiT Lytic Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... 2000 transfected cells were replated into white 384-well tissue culture plates in the presence or absence of 1 μM HaloTag NanoBRET-618 Ligand (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... then luciferase activity was measured using the Dual-Luciferase Reporter assay kit (Promega) on the Glomax instrument (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence intensity of each well and empty controls was measured at 520 nm excitation on a Glomax plate reader (Promega). The average blank control values for each plate were subtracted from individual fluorescence intensity values and weekly measurements were normalized to the initial reading.