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Citations for Promega :
7951 - 8000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and cloned into a pGEM-T Easy Vector System 1 (Promega). Plasmid DNA from ~ 20 positive clones for each cell line was isolated using QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... ChAP assays were performed following transfection with various p53 expression constructs using the Halo-ChIP system (Promega Corporation) according to the manufacturer’s protocol as described previously (Adduri et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... The quantification of NADPH was assessed in total cell lysates using the NADP/NADPH Assay Kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Intracellular ATP levels representing cell growth were measured using the Cell Titer-Glo Luminescent Cell Viability Assays (Promega). ATP concentrations were normalized by cell number ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell Titer Blue assay (Promega, G8080) was performed to access cell viability according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and the total cellular GSH of each cell line was assessed using the GSH-Glo™ Glutathione Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... HUH7 cells were transfected with FuGene 6 (Promega, Madison WI), and cell pellets were harvested 40 hours after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... The total RNA was next reverse-transcribed to cDNA by M-MLV reverse transcriptase (Promega, M1701) 0.5 mg/mL Oligo(dT)15 primers were added to 200 ng of RNA and was incubated at 70°C for 5 mins ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase assay was performed using Dual-Luciferase Reporter assay (Promega, USA) according to the manufacturer’s instructions using Renilla luciferase as internal control after 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... and QuantiFluor RNA System (Promega) respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase activity was detected using Luciferase Assay System (Promega), and normalized to β-gal activity.
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bioRxiv - Cancer Biology 2023Quote: ... genomic DNA was prepared using Wizard genomic DNA purification kit (Promega) as manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured using CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) (Promega) following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and a GloMax 96 Microplate Luminometer (Promega), according to the manufacturer’s standard protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... QuantiFluor® dsDNA System (Promega Corp., Madison, WI, USA), and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... COS-7 cells were plated on 10 cm plates and transfected 24h prior to lysis using FuGENE 6 (Promega; 6-12 µg total DNA). Cells were routinely tested for mycoplasma using a MycoAlert detection kit (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then digested with sequencing grade trypsin (Promega, Madison, WI) at 37 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Dual-Luciferase Assay Kit (E2920, Promega) was used to measure the dual-luciferase activity according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2023Quote: ... Promoter fragments corresponding to wild type and mutant p53 target genes were amplified by standard PCR using DNA from healthy donors and subsequently cloned into the pGL4.2 basic vector (Promega Corporation, Madison, USA) for promoter-luciferase assays ...
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bioRxiv - Cancer Biology 2023Quote: ... and cloned into pFN21A (Promega Corporation, Madison, WI, USA) vector using standard restriction digestion and ligation method ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell lysates were used to determine luciferase activity with the Dual-Luciferase Reporter Assay System (Promega) and a GloMax 96 Microplate Luminometer (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: Lysis was performed in all cell lines with Passive Lysis Buffer (Promega) and then cells were subjected to a freeze-thaw cycle at -80° C to 37° C and centrifuged at maximum speed for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... the 200k pellets were digested for 2 h at 37°C with 0.4 µg of Trypsin/Lys-C (Promega CAT#: V5071) and then overnight by adding 0.4 µg of Trypsin/Lys-C ...
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bioRxiv - Cell Biology 2023Quote: ... Beetle luciferin potassium salt (Promega) was reconstituted in 10mM HEPES with a p.H ...
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bioRxiv - Cell Biology 2023Quote: ... digested overnight at 37°C in the presence of 1.5 µg of sequencing grade modified trypsin (Promega). The resulting peptides were vacuum-dried in a vacuum concentrator to approximately 200 µL ...
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bioRxiv - Cell Biology 2023Quote: ... 25U of RNAsin ribonuclease inhibitor and 500µM dNTP deoxynucleotide mix (all reagents from Promega). Gene expression of CD90 ...
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bioRxiv - Cancer Biology 2023Quote: ... Final clones or pools were authenticated using the PowerPlex® Fusion System (Promega) performed at the Hartwell Center for Biotechnology at St ...
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bioRxiv - Cancer Biology 2023Quote: ... CellTiter-Glo 2.0 (#G9243 Promega) was added to each well in a 1:1 v/v ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid transfections were performed in MDA-T32 cell line using Fugene 6 (Promega). Transfected cells were cultured for over 3 weeks in complete RPMI-1640 medium containing G418 (600 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: Reverse transcription was performed using the Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Madison, WI, USA), 1000 ng of total RNA and random hexamers ...
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bioRxiv - Cancer Biology 2023Quote: ... Reporter experiments were performed using the Dual-Luciferase Reporter Assays (Promega, Madison, WI, USA) following the manufacturer’s protocol and using Renilla (pGL4.73[hRluc/SV40] ...
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bioRxiv - Cancer Biology 2023Quote: ... The optical density was measured spectrophotometrically at 560 nm (Glomax microplate reader, Promega) after 10 h of incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence of GoTaq® Green Master Mix (Promega). For untreated DNA (as controls) ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence of GoTaq® Green Master Mix (Promega). In both cases ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.25 U GoTaq Enzyme (Promega) and 2 mM MgCl2 ...
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bioRxiv - Cancer Biology 2023Quote: Viable cell numbers were measured using CellTiter-Glo Luminescent Cell Viability Assay (Promega) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was quantified with CellTiter-Glo 2.0 reagent (Promega). Plates were equilibrated to room temperature for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... TAMRA-CA was purchased from Promega (G8251). JQ-1 (HY-13030) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1X GoTaq buffer (Promega), 1.25 U GoTaq Enzyme (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Lactate-GloTM and Glutamine/Glutamate-GloTM assay kits were purchased from Promega (Madison, WI). The perforin ELISA kit and Fixable Viability Dye eFluor 780 were purchased from eBioscience (St ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoid viability was developed using CellTiterGlo reagent (Cat #G9683, Promega) and detected using FluoStar Optima Plate Reader (21999 ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability was measured with CellTiterGlo® 3D Reagent (Promega, #G9683).
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bioRxiv - Cancer Biology 2023Quote: ... Viability of organoids was determined after 3 days using CellTiterGlo reagent (Cat #G9683, Promega) and detected using a FluoStar Optima Plate Reader (21999 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were precipitated using chloroform/water/methanol extraction and pellets were digested with Endopeptidase LysC (Wako Chemicals) and sequencing grade modified trypsin (Promega). Peptides were labeled with TMTpro isobaric tags (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... before drying further with a speed vac until the pieces were ‘dice-like bouncy.’ The protein samples were then incubated in digestion buffer [50 mM NH4HCO3 and 10 μg/mL Sequencing grade modified trypsin (Promega)] overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was assessed using the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, G3580, Promega, Madison, WI, USA). In brief ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability was measured by Cell-Titer-Glo-3D (CTG-3D) (Promega, G9683) 14 days after dosing.
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bioRxiv - Cancer Biology 2023Quote: ... Viability was measured by CTG-3D (Promega, G9683) 7 days after dosing ...
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bioRxiv - Cancer Biology 2023Quote: Neutrophils were incubated in triplicate at 100,000 per well in PCa CM with 2x Real-Time Glo reagent (Promega). MT Cell Viability Substrate and NanoLuc® Enzyme were added in equal volumes to culture media to create the 2x Real-time Glo reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... Bangalore using GenePrint 10 (Promega corporation), version 3.0.0 of the SoftGenetics ...