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Citations for Promega :
7751 - 7800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Q-PCR was performed using GoTaq qPCR Master Mix (Promega) with the carboxy-X-rhodamine (CXR ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were extracted with 1XPLB solution provided in the Dual-Luciferase® Reporter Assay Kit (Promega CN: E1910) and transferred to 96-well black plate to be treated with LARII and Stop & Glo components according to protocol of the Dual-Luciferase® Reporter Assay Kit (Promega CN ...
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bioRxiv - Developmental Biology 2023Quote: ... including the identified m6A site (wildtype sequence or A-to-C mutation) was cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega), per manufacturer ’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was completed using Applied Biosystems QuantStudio3 Real-Time PCR system using GoTaq qPCR Master Mix with SYBR Green (Promega). All primers used for RT-qPCR are shown in Table S3 ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 μl suspended streptavidin MagneSphere beads (Promega #Z5481) per sample were washed 3×5 minutes in 0.5x SSC ...
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bioRxiv - Immunology 2023Quote: ... or Lactate-Glo™ Assay (Promega, J5021). Thymocytes were isolated from untreated and TBI-treated mice and incubated in RPMI with 5 mM sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Complementary DNA (cDNA) was generated using 1 μg RNA using M-MLV Reverse Transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... destained and digested in-gel with 200 ng modified trypsin (sequencing grade, Promega) and Rapigest (TM ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed on the plate and incubated with luminescence glo regents per the luminescence glo assay kit’s instructions (Promega). Luminescence was recorded using a BioTek Synergy2 plate reader.
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bioRxiv - Immunology 2023Quote: ... The quantity of RNA was analyzed with the Quantus Fluorometer (Promega, Madison, USA). RNA quality control was done using the 4200 TapeStation System (Agilent Technologies ...
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bioRxiv - Immunology 2023Quote: ... Tissues were embedded in 3% low melting point agar (Promega). Formalin embedding ...
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bioRxiv - Immunology 2023Quote: ... and RNAsin (Promega). Reverse transcription and a first PCR was performed as described above (50 cycles ...
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bioRxiv - Immunology 2023Quote: HTR cells were transfected with the indicated plasmids using TransFast transfection reagent (Promega,Madison, WI, USA) according to the manufacturer’s protocol as previously described [9] ...
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bioRxiv - Immunology 2023Quote: ... and then subjected to overnight digestion at 37°C with sequencing-grade modified trypsin (Promega, Madison, WI, USA). The peptides were extracted ...
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bioRxiv - Immunology 2023Quote: ... Luciferase Assay System (E1501) and Passive Lysis 5 × Buffer (E1941) were purchased from Promega. C1q recombinant protein (A400 ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed with BrightGlow Lysis Reagent (Promega E2620) and the mean relative luminescence units (RLU ...
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bioRxiv - Immunology 2023Quote: ... RNA was treated with RQ1 RNase-Free DNase (Promega) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Lactate dehydrogenase (LDH) release to the culture supernatant was quantified by using the LDH cytotoxicity assay kit (Promega).
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bioRxiv - Immunology 2023Quote: ... Cell cytotoxicity was measured using the CytoTox96 Non-radioactive Cytotoxicity Assay (Promega) and expressed as a percentage of total cellular LDH (100% lysis control) ...
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bioRxiv - Immunology 2023Quote: Proliferation of MC38 cells was measured using the CellTiter-glo 2.0 Assay (Promega) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and 60 U/mL RNasin-Plus RNase Inhibitor (Promega N2611). A 5% IGEPAL-630 solution was added to bring the homogenate to 0.3 % IGEPAL CA-630 ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed in Bright-Glo Luciferase Buffer (Promega) diluted 1:1 with PBS and 1% NP-40 ...
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bioRxiv - Immunology 2023Quote: ... on the Quantus Fluorometer (Promega). All RNA samples had a RIN score above 8 ...
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bioRxiv - Immunology 2023Quote: ... using the luciferase assay system with the Passive Lysis 5X buffer (Promega) according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Luciferase assays were performed with the dual-luciferase reporter assay kit (Promega, Madison, WI, USA). Transfection efficiency for the MNRR1 reporter was normalized with the co-transfected pRL-SV40 Renilla luciferase expression plasmid [10,12,13] ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μL of Dual-Glo Stop & Glo Reagent (Promega) was added to each well and incubated for an additional 10 mins at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Caspase 3/7 activity was quantified in cell culture by using the Caspase-Glo 3/7 assay system (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... We then reversed transcribed RNA into cDNA by using Improm II reverse transcriptase (Promega, Madison, WI) and random hexamer primers (Genelink ...
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bioRxiv - Cell Biology 2023Quote: Target sequence containing either P.E22G or P.F21C mutant SNPs were cloned into the multiple cloning site (MCS) of luciferase reporter vector pmirGLO (Promega) between the NheI and SalI restriction enzyme (RE ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured for 24 hours and luciferase activity was quantified using the Dual-Glo Luciferase Assay System (Promega) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in 50 μL Passive lysis buffer (Promega) in which 20 μL of lysate was mixed with 20 μL of Dual-Glo Luciferase Reagent and incubated for 10 mins at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of sequencing-grade trypsin (Promega) and 150 μL of digestion buffer (50 mM TEAB ...
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bioRxiv - Cell Biology 2023Quote: ... FuGENE HD Transfection Reagent (E2311, Promega), Oligomycin A (75351 ...
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bioRxiv - Developmental Biology 2023Quote: ... Probe is then revealed with NBT/BCIP (Promega). Leg discs were dissected and mounted in a mix PBS/Glycerol.
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse IgG HRP conjugate (Promega) anti-rabbit rhodamine–conjugated tubulin (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... and M-MLV reverse transcriptase (Promega). Quantitative PCR was performed with iTaq Universal SYBR green supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... and viability (that was not affected) was measured using cell-titer glo (Promega) to ensure there were no viability differences (>10% ...
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bioRxiv - Cell Biology 2023Quote: ... Luminescence was measured 24-36 hours after transfection using Nano-glo (Promega) and viability (that was not affected ...
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bioRxiv - Developmental Biology 2023Quote: ... The modified proteins were digested with lysyl endopeptidase (Lys-C) (Wako) for 3h at 37° C and subsequently digested with trypsin (Promega) overnight at 37° C ...
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bioRxiv - Developmental Biology 2023Quote: ... dNTP mix (2.5 mM each, Promega), ERCC RNA Spike-In Mix (1 ...
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bioRxiv - Developmental Biology 2023Quote: ... LacZ reporter activity was assayed in defined media (SD) supplemented with -Leu-Trp using the Beta-Glow® Assay System following commercially available protocols (Promega #E4720). In short ...
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bioRxiv - Developmental Biology 2023Quote: Notch reporter 4xcsl::H2B-mvenus plasmid(Nowotschin et al., 2013) was injected at 30ng/µl after purification using the PureYield Midiprep kit (Promega, #A2492). For antisense in situ hybridization probes ...
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bioRxiv - Developmental Biology 2023Quote: ... using the T7/T3 Riboprobe Combination System kit (Promega) and the Digoxigenin labeling kit (Roche) ...
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bioRxiv - Developmental Biology 2023Quote: ... and synthesized with SP6 RNA polymerase (Promega #P108G) or Sac1 (New England BioLabs #r3156S ...
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bioRxiv - Developmental Biology 2023Quote: ... and SYBR Green fluorescent dye (Promega, Madison, WI, USA) were used ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse monoclonal antibIII tubulin (1:500, Promega, #G712A), mouse anti-tubulin bIII (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were treated with DNase I (Promega) at 37degC ...
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bioRxiv - Cell Biology 2023Quote: ... all the steps were done in the presence of RNase inhibitor (recombinant RNasein, Promega, N2511) diluted 1:4,000 for washing steps or 1:400 for incubation while staining and for buffers in the tubes containing the sorted cells followed by snap freeze and storage at –80°C.
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bioRxiv - Cell Biology 2023Quote: Lysates were digested from the beads with 100 ng of LysC (Promega) in 25mM HEPES pH 8.5 overnight at 37 °C with shaking at 1200 RPM in a thermomixer ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs were prepared using the Ambion mMessage Machine kit using Sp6 (#AM1340) supplemented with RNAse Inhibitor (Promega #N251B) after plasmid linearization with Not1 ...