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Citations for Promega :
7601 - 7650 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and pTK-renilla luciferase plasmid was from Promega.
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37 °C for 24 h and then they were washed with 200 μL PBS per well and harvested in 100 μL per well of passive lysis buffer (Promega). Cells were freeze-thawed and 25 μL of lysate from each well was added to 25 μL of firefly luciferase reagent (Bright-Glo ...
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bioRxiv - Microbiology 2023Quote: ... Measurement of luciferase activity was carried out using a Glomax Navigator Microplate Luminometer (Promega). In all experiments ...
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bioRxiv - Microbiology 2023Quote: ... Cells were freeze-thawed and 25 μL of lysate from each well was added to 25 μL of firefly luciferase reagent (Bright-Glo, Promega), and 10 μL of each lysate was added to 50 μL of 2 μg/mL Renilla luciferase substrate (NanoLight Technology) ...
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bioRxiv - Microbiology 2023Quote: ... Luciferase activity was measured 36 h post-transfection using the Dual-Glo luciferase assay system (Promega) by using the Glomax Navigator Microplate Luminometer (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The Renilla reporter plasmid pRL-TK (Promega) was co-transfected as a control for transfection efficiency ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting proteins with carbamidomethyl groups on their cysteines were digested with 300 ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA) and injected on an Easy-nanoLC-1000 system coupled to a Q-Exactive+ mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... A volume of cells equivalent to 1 mL at OD600 = 0.2 was centrifuged and re-suspended in 1ml fresh medium supplemented with JF549 (Janelia Fluor®HaloTag®Ligands, Promega) at a final concentration of 1 µM ...
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bioRxiv - Molecular Biology 2023Quote: SMC5/6 hexamer purified from E.coli with a C-terminal HALO tag on Nse2 was incubated with a 2-fold molar excess of Janelia-Fluor646 HaloTag Ligand (Promega; 12 µM Smc5/6 hexamer + 24 µM label). After incubation for 1 h at 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNase inhibitor (Promega) in DEPC treated water ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized with Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI). qRT-PCR was performed with the BioRad CFX96 Real-Time PCR System or Applied Biosystems 7000 ...
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bioRxiv - Microbiology 2023Quote: After extraction with the Wizard® Genomic DNA Purification Kit (Promega), all isolates in this study were sequenced ...
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bioRxiv - Biochemistry 2023Quote: ... HALO (Promega, G9211). For quantification ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was measured with CellTiter (Promega) and the absorbance was read at 490 nm with the plate reader GloMax® Discover instru-ment ...
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bioRxiv - Immunology 2023Quote: In situ nick-end labeling of nuclear DNA fragmentation was performed with a TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL system, Promega) according to the supplier’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Dual Luciferase Reporter Assay System (Promega) was used to measure the intensities of Gaussia and Firefly luciferases ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega) in accordance with manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... FuGENE HD (Promega-E2313), mouse TNF-α ELISA kit (Invitrogen-88-7324-88) ...
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bioRxiv - Microbiology 2023Quote: LDH assays were performed with the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... One μg of sequencing grade trypsin (Promega) was then added per sample and digestion was carried out overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... transfected cells were treated with human IFN-γ at (100 U/mL for 24 hr) and firefly luciferase activity was determined using the dual luciferase reporter assay system (Promega) on SpectraMax iD3 (Molecular Devices ...
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bioRxiv - Microbiology 2023Quote: ... which was performed with the M-MLV Reverse Transcriptase (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Fresh MEM supplemented with 200 μM GCDCA was then added on the cells in the presence or absence of Z-VAD-FMK (Promega), NH4Cl (Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... We used 1 ml of cultures and isolated the genomic DNA using the Wizard Genomic DNA Purification Kit (Promega; Madison, WI). We used the DNA with the Illumina DNA Prep kit (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... All the DNA concentrations were identified by the QuantiFluor dsDNA system (Promega, USA). The library products were sequenced on the DNBSEQ-T7 DNA system (MGI Tech ...
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bioRxiv - Cancer Biology 2023Quote: ... 100uL of CellTiter-Glo Reagent (Promega Corp., Catalog # G7572) was used to lyse the cells and measure cell death at Timepoint 0 for the non-irradiated 2D and 3D HCC827 plates ...
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bioRxiv - Immunology 2023Quote: ... Tol2-mpx:mCherry-2A-rac2 [43] (a gift from Anna Huttenlocher) was cut with NheI and SalI (Promega) to remove the mpx:mCherry-2A-rac2 insert ...
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bioRxiv - Immunology 2023Quote: Proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Focuses of infection were revealed using NBT/BCIP reagent (Promega), following the manufacturer’s instructions and the virus titer calculated as follow ...
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bioRxiv - Neuroscience 2023Quote: Approximately 5x104 HEK293 cells were seeded on poly-L-lysine coated glass coverslips and transfected with expression constructs for SORL1-WT or SORL1-R953C using Fugene 6 Transfection Reagent kit (Promega). 24h post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... using Fugene 6 Transfection Reagent kit (Promega) according to manufacturers’ instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were lysed with Luciferase Cell Culture Lysis Reagent (Promega) and luciferase activity was measured with Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligation of the digested pGEM-T easy vector and 5’ fragments was carried out overnight at 4 °C using 0.5 µl (1.5 U) of T4 DNA Ligase (Promega). pGEM-T easy vector containing the 5’ fragment (5’-pGEM-T ...
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bioRxiv - Molecular Biology 2023Quote: RNA samples from two biological replicates were pooled and total RNA was treated with DNase (1 U/µl, Promega). For cDNA synthesis ...
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bioRxiv - Immunology 2023Quote: ... pGL4.32[luc2P/NFκB-RE/Hygro] was from Promega. Antibodies were from Novus (α-HP JM10-79) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from embryo composite samples or adult tissue using ReliaPrep RNA Tissue Miniprep System (Promega). Reverse transcription was performed on RNA to produce cDNA for RT-PCR using an iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase-3 and −7 activity was measured following three days of treatment (Caspase-Glo #G8093, Promega), while cell viability was obtained after six days of treatment (CellTiter-Glo 3D #G9681) ...
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bioRxiv - Developmental Biology 2023Quote: ... and then 5 ng/μL of trypsin (Promega) was added and samples incubated over night at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells expressing the transporters were additionally transfected with a plasmid coding for NanoLuc (N1411, Promega, Madison, WI) and selected with 40 μg/ml hygromycin B (InvivoGen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NanoLuc (N700, Promega), or β-actin (Rabbit 4970L ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RealTime-Glo MT Cell Viability Assay (Promega, cat # G9711) was used to quantify cellular reducing ability according to manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... the luminescent signal was measured weekly by adding 1.5 mg/ml Beetle luciferin (E1601, Promega) for 48h to the neurospheroid cultures ...
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bioRxiv - Neuroscience 2023Quote: ... Dual-Glo Luciferase Assay System (Promega) was used to measure Firefly luciferase (FFluc ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction contained 0.25 µl Taq Polymerase (Promega), 2.5 µl 10 uM forward and reverse primers ...
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bioRxiv - Molecular Biology 2023Quote: Luciferase assays were performed using the Dual-Luciferase® Reporter Assay Kit (Promega, E1960). N2a cells were planted in 96-well plates at 2.0 x 104 cells/well ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA/Protein Purification Plus kit (Norgen, Thorold, ON, Canada) or the Maxwell 16 LEV simplyRNA purification kit (Promega, Madison, WI, USA) according to the manufacturers’ instructions ...
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bioRxiv - Physiology 2023Quote: ... cDNA was prepared according to the manufacturer’s protocol using the ImProm-II reverse transcription system (Promega, Madison, WI). Quantitation of gene expression was performed by real-time RT-PCR Quant Studio 3 (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 µL coelenterazine h (Promega, 5 µM final concentration) was added ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cell viability was evaluated using the CellTiter-Glo assay (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the plates were subjected to Nano-Glo HiBiT Lytic detection system (Promega, #N3040) as described in manufacturer’s manual ...