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Citations for Promega :
8001 - 8050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 300 µL of 100 mM NH4HCO3 with sequencing grade porcine trypsin (Promega) at a trypsin-to-protein ratio of 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... or FuGENE HD (Promega, cat. no. E2311) for A2058 cells ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were diluted 3 fold with 20mM HEPES pH 8.0 and digested in 10 @g ml-1 trypsin-TPCK (Promega) overnight at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... H9 hESC harbouring the mitochondrial matrix mCherry-GFP flux reporter were generated by transfection of 1×105 cells with 1μg pAC150-PiggyBac-matrix-mCherry-eGFPXL (Harper’s lab) and 1μg pCMV-HypBAC-PiggyBac-Helper(Sanger Institute) in conjunction with the transfection reagent FuGENE HD (Promega). The cells were selected and maintained in TeSR™-E8™ medium supplemented with 200 mg/ml Hygromycin and Hygromycin was kept in the medium during differentiation to iNeurons ...
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bioRxiv - Cell Biology 2023Quote: ... cells and cerebellum collected with Passive Lysis Buffer (Promega), were analyzed with the addition of luciferin (100mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to induce gene expression and cell viability was measured using cell titer glow 3D using manufacturer’s instructions (Promega) on Day7 and Day10 ...
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bioRxiv - Cancer Biology 2023Quote: ... spheroids were treated with alpelisib and/or tipifarnib and baseline growth measured using 3D Cell Titer Glo reagent (Promega). Spheroids were incubated with drug for 7 days and a final CTG reading taken ...
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Cell Biology 2023Quote: Total cellular ATP levels were measured using a bioluminescent ATP assay Kit (Promega, # G755A, G756A) as described in the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were successively lysed and assayed by a 2-DG uptake assay kit (J1341, Promega) according to the instruction ...
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bioRxiv - Cell Biology 2023Quote: Mouse genetic Hnf4a promoter (-2000 to 0) and its deletion mutants (-350 to 0) were cloned into pGL4.14 vector (E6691, Promega) by Gibson Assembly kit (A46624 ...
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bioRxiv - Cell Biology 2023Quote: ... The luciferase assay was performed according to the manufacturer’s instruction (E1500, Promega).
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bioRxiv - Cell Biology 2023Quote: ... 10 μl of lysate was incubated with 50 μl luciferase reagent (Promega) and measured for 10 s using (Lumat LB9507 ...
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bioRxiv - Cell Biology 2023Quote: Lysates for luciferase assay were prepared in 1× passive lysis buffer (Promega), 100 μl per well of a 24-well plate ...
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bioRxiv - Cell Biology 2023Quote: SMC4 was stained using HaloTag TMR Ligand (Promega, G8251) in control and ΔSMC4 cells for 30 min (1:1000 dilution in wild type medium) ...
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bioRxiv - Cell Biology 2023Quote: ... and the luciferase activity was determined using the dual luciferase assay system (E1500, Promega) according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... Fugene6 HD (#E2691, Promega), lipofectamine 2000 (#11668019 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell viability was measured in triplicates using the CellTiter-GloR Luminescence assays from Promega or by counting live cells using Countess 3 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was analyzed by RT-qPCR using SYBR green fluorescent dye 2x qPCR master mix (Promega) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-rabbit HRP (Promega) or anti-mouse HRP (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... 10 pg luciferase mRNA (Promega) was spiked into each fraction as a recovery control ...
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bioRxiv - Cell Biology 2023Quote: ... CellTiter-Glo® Luminescent Cell Viability Assay (#G7570, Promega). Methyl metanosulfonate (MMS ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were lysed 24 h after transfection and luciferase activity was measured with the Dual-Luciferase Reporter Assay kit (Promega Cat. # E1960) according to the manufacturer’s protocol using a FlUOstar plate reader (BMG Labtech) ...
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bioRxiv - Cell Biology 2023Quote: ... The pmiRGLO plasmid (Promega)-based miR-20 ...
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bioRxiv - Cell Biology 2023Quote: ... HaloTag® TMR ligand (Promega) was added to the cell suspension at the final concentration of 1 nM during the last 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Trypsin/LysC (Promega) was added twice at 1:100 (wt/wt ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed 24 h after transfection and luciferase activity was measured with Dual-Luciferase Reporter Assay (Promega Cat. # E1960) according to the manufacturer’s protocol using a FlUOstar plate reader (BMG Labtech) ...
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bioRxiv - Biophysics 2023Quote: ... Then the gel was dried using a gel drying kit (Promega) and exposed to a phosphor screen (overnight) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1:400 RNase inhibitor (Promega cat. #: N251B). Protein lysates were serially diluted to concentrations of 0.6 μg/μL ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified using GoScript™ Reverse Transcription System (Promega) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were validated by DNA fingerprinting through the University of Colorado Molecular Biology Service Center utilizing the STR DNA Profiling PowerPlex-16 HS Kit (DC2101, Promega)(Extended Data Figure 11).
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was assessed by CellTiterGlo® (Promega). The viability effect of each compound was calculated for each clone ...
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bioRxiv - Cancer Biology 2023Quote: ... the RNasin Plus RNase inhibitor and random primers (all from Promega). SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: The 3-dimensional (3D) culture studies were performed using MCF-7 cells and low melting point agarose (Promega, Australia). Agarose was suspended in PBS at a concentration of 0.75% (w/v) ...
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bioRxiv - Biophysics 2023Quote: 5 μM purified EGFP-YbbR-Lphn3 GAIN-HaloTag protein in HBS was mixed with 5.2 μM HaloTag PEG-Biotin Ligand (Promega, G8592) and incubated at room temperature for 2 hours to biotinylate the CTF of the GAIN construct ...
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were co-transfected with FLAG-tagged receptor (3.5μg) and luciferase-based 22F cAMP biosensor construct (3.5μg) (Promega, Cat. no: E2301) using polyethyleneimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µl CellTiterGlo® (Promega) per well was added and the luminescence was read at 700 nm ...
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bioRxiv - Cell Biology 2023Quote: Cells expressing one plasmid were transfected with FuGENE 6 (Promega #E2691) at a FuGENE:DNA (μL:μg ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified DNA fragments were purified using Wizard SV Gel and the PCR Clean-Up System (Cat. N° A9281 Promega), then the DNA was sequenced using the Macrogen facility (https://dna.macrogen.com/).
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bioRxiv - Cell Biology 2023Quote: ... ligation of double digested fragment of either domain with double digested pAcGFP1-N1 was performed with the LigaFAst Rapid DNA Ligation System (Promega, M8226) following the manufacturer’s suggested protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Ift122 cDNAs were amplified using GoTaq Green (Promega, Madison, WI, USA) and the primers ...
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bioRxiv - Biophysics 2023Quote: ... Luminescence was measured using GM-2000 or GM-3510 microplate reader (Promega). Luminescence level of each well was measured every 30 sec with integration time of 0.9 sec ...
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bioRxiv - Biophysics 2023Quote: ... Luminescence was measured using GM-2000 or GM-3510 microplate reader (Promega). Luminescence level of each well was measured every 6 sec with integration time of 0.3 sec ...
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bioRxiv - Cell Biology 2023Quote: ... and measurement of Firefly and Renilla Luciferase activity was performed using the Dual-Luciferase Reporter Assay System (E1960, Promega) in a GLOMAX microplate luminometer ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µL of ADP Glo Reagent (Promega), supplemented with 0.01% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... pGL4.13[luc2/SV40] vector (#E6681, Promega) served as a positive control ...
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bioRxiv - Cancer Biology 2023Quote: ... mixed with GSH Assay Mixture and GSH was calculated by measuring in a microplate reader (Promega GloMax) using 490/520 nm excitation/emission filter and fitting into a GSH standard curve.
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was spun onto an S-Trap device (Protifi, Farmingdale, NY) and digested by 750 ng trypsin (Promega, Madison, WI) in 100 mM TEAB buffer at 37°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...