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Citations for Promega :
7901 - 7950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... or 25 µL of prepared Nano-Glo Luciferase Assay System (Promega # N1120) and detected as described above ...
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bioRxiv - Cell Biology 2023Quote: ... with trypsin (Promega). The digested peptides were labeled with 10-plex TMT (Thermo product 90309 ...
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bioRxiv - Neuroscience 2023Quote: ... Following manufacturer’s instruction (Promega), neuronal cultures at DIV8 were washed three times with PBS and lysed in buffer containing 25 μl PBS and 25 μl 1 % DTAB (Dodecyltrimethylammonium bromide ...
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bioRxiv - Biochemistry 2023Quote: ... and 2 µg of bacmid DNA were used to transfect Sf21 cells for baculovirus generation using FuGENE® transfection reagent (Promega). The protein was expressed in Lonza Insect-EXPRESS™ medium for 72-96 h ...
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bioRxiv - Biochemistry 2023Quote: ... and digested with 0.5 μg of trypsin (Sequencing Grade, Promega, or Pierce) in 50mM HEPES (pH 8.0 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and reverse transcription was performed using GoScriptTM Reverse Transcription System (Promega, USA). Target SnRK2 (III ...
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bioRxiv - Evolutionary Biology 2023Quote: The total RNA of Lemna minor was extracted using Eastep® Super Total RNA Extraction Kit (Promega, USA) and reverse transcription was performed using GoScriptTM Reverse Transcription System (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were transfected into HEK293T cells using FuGENE HD (Promega, E2312), and proteins were allowed to express for 20 h ...
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bioRxiv - Biochemistry 2023Quote: ... Serially diluted inhibitor and the corresponding NanoBRET Kinase Tracer (Promega) at a concentration determined previously as the Tracer IC50 (Table S3 ...
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bioRxiv - Biochemistry 2023Quote: ... In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc fusion (Promega) as specified in Table S3 ...
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bioRxiv - Biochemistry 2023Quote: ... NanoBRET NanoGlo Substrate + Extracellular NanoLuc Inhibitor (Promega, N2540) was added as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and peptides were generated by in-gel digestion using trypsin (sequencing-grade (Promega)) at 37°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was digested overnight at 37 °C with one or more of the following enzymes separately: trypsin (Promega), Lys-C (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... and Dounce homogenized in 500 µL of ice-cold RNase-free buffer polysome buffer (50 mM Tris pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% NP-40, 1 mM DTT, 200U/ml Promega RNasin ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were proteolysed for 2 h at room temperature using 0.5 μg Lys-C (Wako) and subsequently for 16 h at 37 °C using 1 μg trypsin (Promega). Peptides were collected by centrifugation and acidified with 0.5 % trifluoroacetic acid.
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bioRxiv - Biochemistry 2023Quote: ... The luciferase activity was measured with ONE-Glo Luciferase reagent (Promega) with Analyst via a HT luminescence reader.
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bioRxiv - Cancer Biology 2023Quote: ... and matched adjacent normal tissues were prepared using the Total RNA Extraction Kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The band corresponding to TP53INP2 was excised and digested with sequencing grade modified trypsin (Promega), using LFASP digestion protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and anti-mouse IgG alkaline phosphatase conjugated antibody (Promega). Band intensities were quantified by Image J ...
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bioRxiv - Biochemistry 2023Quote: ... The luminescence was measured immediately after adding 20µl NanoGlo substrate by using a GLOMAX luminometer from Agilent Technology (Promega) (Fig.1e)
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bioRxiv - Biochemistry 2023Quote: ... Cells were grown to 80% confluency prior to transfection with the indicated FLAG tagged ISG15 pcDNA5 plasmids using the FuGene transfection reagent (Promega). After 24 hr ...
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bioRxiv - Biochemistry 2023Quote: ... The amplified fragment was subcloned in pGEM®-T Easy (Promega, Madison, WI, US) and sequenced (Genwiz ...
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bioRxiv - Immunology 2023Quote: ... we used the Bright-Glo Luciferase Assay System (Promega, E2610) to measure relative light units (RLUs ...
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bioRxiv - Genetics 2023Quote: ... cell lysates were collected and luminescence measured using the Dual-Glo luciferase system (Promega E2920) on the GloMax Discover plate reader (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was synthesized from each reaction mixture using the GoScript Reverse Transcription System (Promega) per the manufacturer’s protocol.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... samples were digested with 0.1 μg of trypsin/Lys-C mix (Promega) at 37 °C for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... and digestion of purified crosslinked protein with sequencing grade modified porcine trypsin (Promega), tryptic peptides were separated by reverse phase XSelect CSH C18 2.5 um resin (Waters ...
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bioRxiv - Biochemistry 2023Quote: ... using the GoTaq® qPCR Master mix (Promega, Madison, WI, USA). Bio-Rad CFX Manager software was used for data acquisition and analysis (version 3.1 ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids pKHNH6 and pKHNH3 were linearised prior to transformation using restriction enzymes EcoRV (Promega, USA) and KpnI (Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... and caspase-8 activity was measured using the Caspase-Glo® 8 kit (Promega), which uses the specific caspase-8 substrate LETD sequence ...
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bioRxiv - Cancer Biology 2023Quote: ... they were treated for the indicated times and viability was assessed using the CellTiter-Glo® 3D Cell viability kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 5% polyacrylamide TBE gel and stained with Diamond DNA stain (Promega). Experiments were performed with H ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained with Diamond DNA stain (Promega) and the disappearance of the NCP band quantified using ImageLab ...
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bioRxiv - Biochemistry 2023Quote: ... prior to running on a denaturing urea 10% polyacrylamide gel and stained with Diamond DNA stain (Promega). The experiment was repeated in triplicate and quantified using BioRad Image Lab software.
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bioRxiv - Biochemistry 2023Quote: ... Samples were further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibodies: anti-rabbit IgG HRP conjugate (Promega, catalog no ...
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bioRxiv - Biochemistry 2023Quote: 20 µl of CellTiter-Glo (CTG) luminescent solution (Promega) was added in each well after equilibration of the 96 well plate and CTG solution at room temperature for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... anti-mouse IgG HRP conjugate (Promega, catalog no ...
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bioRxiv - Biochemistry 2023Quote: ... and then digested with trypsin (Mass Spectrometry grade, from Promega Corp., Madison, WI, USA) overnight ...
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bioRxiv - Immunology 2023Quote: ... the Bio-GloTM luciferase assay system was applied to estimate the T-cell activation according to the manual (Promega, Madison, WI). The relative luminescence unit (RLU ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl were transferred into a white flat bottom 96 well plate in triplicates and 50 μl of Promega NanoGlo Luciferase assay reagent (Promega) prepared according to the manufacturer’s instructions was added to the wells ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant His-MBP-AlpA and His-MBP at a concentration of 10 μM was incubated with 100 μl MagneHis™ Ni Particles (Promega) equilibrated with pulldown-buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... HiBiT-tagged proteins were detected using the Nano-Glo HiBiT blotting system (Promega) according to the manual ...
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bioRxiv - Microbiology 2023Quote: ... gallolyticus genomic DNA was extracted using either Wizard Genomic DNA Purification Kit or Wizard SV Genomic DNA Purification Kit (Promega). The extraction was performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The solubilized membrane proteins were subsequently supplemented with imidazole to a final concentration of 50 mM and incubated with nickel-immobilized HisLink resins (Promega) that had been pre-calibrated in Buffer B (100 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were diluted 1/100 with the extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay (Promega, RRID:SCR_006724)) ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Biochemistry 2023Quote: ProAlanase (mass spec grade) was ordered from Promega. Expression plasmids for recombinant GmPOPB (plasmid PMA 1237 ...
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bioRxiv - Biochemistry 2023Quote: ... and 40 U/µL RNasin™ Plus (Promega, N2615). T7 (P266L ...
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bioRxiv - Biochemistry 2023Quote: ... One µl of 0.1 mM HaloTag NanoBRET ligand 618 (Promega, Madison, WI, USA) per ml of cells ...