Labshake search
Citations for Roche :
3801 - 3850 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... they were quantified on a CFX 384 Touch Real-Time PCR Detection System using the KAPA Library Quantification Kit (KAPA Biosystems, cat. KK4824). Finally ...
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bioRxiv - Microbiology 2024Quote: ... samples were collected and plasma HIV RNA levels tested by quantitative rRT-PCR (Roche Cobas Ampliprep/Cobas Taqman HIV-1 test, Roche Diagnostics, Mannheim, Germany) at the NICD.
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bioRxiv - Cell Biology 2023Quote: ... probe was amplified by PCR as described previously (Cox et al., 1993) and labeled using the biotin-nick translation mix (Roche Applied Science, # 11745824910), according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... Digoxigenin-labeled antisense and sense RNA probes were produced from cloned PCR fragments using a digoxigenin RNA labeling kit (SP6/T7; Roche Diagnostics, Basal, Switzerland). Labeling was performed as previously described (Ukena et al. ...
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bioRxiv - Microbiology 2023Quote: The absolute abundance of the 16S rRNA gene was measured on LightCycler® Roche 480 Real-time PCR instrument (Roche Inc., Basel, Switzerland). A total of 20 μL reaction system consisted of 10 μL LightCycler® 480 SYBR Green I Master Mix (2 × ...
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bioRxiv - Genetics 2023Quote: ... AB individuals with the shortest wild introgressed fragment were selfed and the progeny was genotyped by High Resolution Melting (HRM) on a LightCycler 480 Real-Time PCR (Roche Diagnostics, Meylan, France) to identify recombinants with shorter introgressions ...
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bioRxiv - Immunology 2023Quote: ... and adapter-ligated using the KAPA PCR-free Hyper Prep Kit in combination with KAPA Unique Dual-Indexed Adapters (F. Hoffmann-La Roche AG, Basel, Switzerland). Adapter-ligated libraries were purified by AMPure XP bead cleanup ...
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bioRxiv - Genetics 2023Quote: ... The sequencing library was then validated on the Agilent TapeStation and quantified using a Qubit 2.0 Fluorometer in addition to quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). The sequencing libraries were multiplexed and clustered onto two flowcells ...
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bioRxiv - Genomics 2023Quote: ... The concentration of each library was determined by quantitative PCR (qPCR) via the KAPA Library Quantification Kit for Next Generation Sequencing (KAPA Biosystems; Woburn, MA).
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bioRxiv - Plant Biology 2023Quote: ... 50 ng cDNA were used for each technical triplicate and the PCR reaction was performed in a LightCycler® 480 (Roche, Basel, Switzerland). -RT and water controls for each primer pair were included ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified DNA was quantified using GeneAce SYBR qPCR Mix α (NIPPON GENE, Tokyo, Japan) combined with a LightCycler 96 Real-Time PCR system (Roche, Basel, Switzerland). The primers used are listed in supplemental table (Table ...
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bioRxiv - Plant Biology 2023Quote: ... Southern blotting was performed with the digoxigenin (DIG)-labelled PCR-amplified gene fragment of HPT probe as described in the DIG System Manual (Roche, Inc., Basel, Switzerland). Genomic DNA (20 μg ...
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bioRxiv - Cancer Biology 2023Quote: ... Total yield of around 40 ng was split in two and PCR amplified using 2X KAPA HiFi Hot-Start ReadyMix (Roche, cat. no. KK2602) and KAPA Library Amplification Primer Mix (10X concentration ...
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bioRxiv - Microbiology 2023Quote: ... 0.09 ng of cDNA per well was used in qRT-PCR with KAPA SYBR® FAST qPCR Master Mix Kit (2×) (KAPA Biosystems, USA) on an Applied StepOnePlusTM Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was performed using Hieff qPCR SYBR® Green Master Mix (Yeasen, China) in LightCycler® 96 real-time PCR instrument (Roche, China), followed the instructions.
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription and real-time qPCR reaction were carried out with KAPA SYBR FAST One-Step qRT-PCR Master Mix Kit (KAPA Biosystems, Wilmington, MA) on LighyCycler 480 system (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then amplified the adapter-ligated libraries with indexing oligos (Glenn lab, University of Georgia) and the KAPA HiFi PCR Kit (Roche, Indianapolis, Indiana, USA) using 16 cycles of PCR ...
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bioRxiv - Neuroscience 2024Quote: ... The physical vector titers were quantified by measuring the number of packaged vector genomes by real-time PCR using SYBR Green reaction mix (Roche Diagnostics, Mannheim, Germany).
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bioRxiv - Physiology 2024Quote: Gene expression from the 1V-2V region of cutC was performed by quantitative PCR (qPCR) in a LightCycler 480 (Roche Diagnostics, Mannheim, Germany) using the Maxima SYBR Green/ROX qPCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... the cDNAs of RBP (head/thorax) of individual mosquitoes composing each pool were screened by real-time PCRs on a LightCycler® 480 (LC480) (Roche Applied Science, Germany). Real-time PCR assay targeting the virus of interest (see primers/probe sets in Table 7 ...
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bioRxiv - Systems Biology 2019Quote: ... The reactions were performed in triplicates in white FrameStar® 384 PCR plates (4titude) on a LightCycler® 480 system (Roche Diagnostics, Meylan, France) and gene expression analysis was performed with the use of qBase [40] ...
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bioRxiv - Biochemistry 2021Quote: Quantitative RT-PCR was performed on the Roche LightCycler 480 Instrument II system using the LightCycler 480 SYBR Green I Master (Roche Applied Science, Mannheim, Germany). Template cDNA was diluted 1:10 ...
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bioRxiv - Microbiology 2021Quote: ... First-strand cDNA synthesis and quantitative real-time PCR were performed with KAPA SYBR® FAST (CliniSciences, Nanterre, France) on the LightCycler 480 (Roche Diagnostics, Meylan, France) using the primers indicated in Supplementary Table 4 ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were pooled in equimolar amounts after library quantification using both quantitative PCR with the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA) and the fluorometric Qubit dsDNA high sensitivity assay kit (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: DNA protein interaction was studied with purified H-NS and DIG labeled PCR amplified PctxAB fragments by using the DIG gel shift kit (2nd Gen, Roche Aplied Science, Mannheim, Germany) as previously described (10) ...
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bioRxiv - Microbiology 2019Quote: ... DNA fragments encompassing TPP riboswitches and the first 5 codons of the downstream gene were amplified from genomic DNA by PCR using HiFi Taq MasterMix (KAPA Biosystems, Wilmington, MA, USA) and inserted in frame preceding the nanoluciferase gene in the pNBU2 vector ...
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bioRxiv - Cancer Biology 2020Quote: ... cell lysates were prepared as described previously [17] Quantitative detection of mRNA was performed by real-time PCR using the Lightcycler 480 detector (Roche Applied Science, Manheim Germany) as previously published [17].
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bioRxiv - Cell Biology 2020Quote: ... qPCR was conducted using Roche SYBR-Green® master mix and a LightCycler 480 II Real-Time PCR system (Roche Applied Science, Penzberg, Germany). For analysis ...
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bioRxiv - Physiology 2019Quote: ... Libraries were pooled in equimolar amounts after library quantification using both quantitative PCR with the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA) and the fluorometric Qubit dsDNA high sensitivity assay kit (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: All quantitative PCR (qPCR) reactions were performed in duplicates and conducted in 96 well plates on LightCycler® 480 system (Roche Diagnostics, Mannheim, Germany). Each reaction consisted of a total amount of 12 µL divided into 6 µL LightCycler 480 SYBR Green I Master (Roche Diagnostics) ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCR assays were performed in a final volume of 12 µL using the LightCycler® 480 Probe Master Mix 1X (Roche Applied Science, Germany) with primers and probes at 200 nM and 2 µL of control cDNA (virus reference material ...
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bioRxiv - Genetics 2020Quote: ... The final linked-read library (8 cycles of PCR amplification) was quantified using the Kapa Library Quantification Kit (Cat. No. KK4824) from Kapa Biosystems (Woburn, MA, USA) and diluted to 4 nM ...
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bioRxiv - Microbiology 2020Quote: ... HBV DNA (rcDNA and cccDNA) and RNA (pregenomic RNA, pgRNA) were quantified by real-time PCR on a LightCycler™ instrument (Roche Diagnostics, Mannheim, Germany) using specific PCR primers as described (Lucifora ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR reactions were performed using the LightCycler® 480 SYBR Green I Master Mix with a LightCycler 480 instrument (Roche Applied Science, UK) under the following conditions ...
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bioRxiv - Genetics 2020Quote: ... Tag sequences for identifying individual trees were attached to the DNA fragments using the Access Array Barcode Library for Illumina Sequencers-384, Single Direction (Fluidigm Corporation, South San Francisco, CA, USA) and KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BioSystems, Wilmington, MA, USA). PCR was performed in a 20 μL volume containing 2 μL of 20-times diluted PCR products ...
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bioRxiv - Genetics 2022Quote: ... The hygromycin cassette including the B-tubulin promoter and terminator was amplified from pPHT1 plasmid with the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, MA, USA). The PCR thermocycling profile used was as follows ...
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bioRxiv - Microbiology 2022Quote: ... The KSHV genome-enriched CHi-C library DNA was eluted and PCR enrichment (12 cycles) performed with high-fidelity KAPA HiFi HotStart DNA Polymerase (Kapa Biosystems, Inc., Wilmington, MA). Libraries were multiplex sequencing (2 × 150 bp ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative RT-PCR was performed in technical triplicates using SYBR Green I Master Mix in a LightCycler 480 instrument (both from Roche Diagnostics, Indianapolis, IN, USA). Primers for RT-qPCR are listed in Supplementary Table S1.
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bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...
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bioRxiv - Immunology 2023Quote: ... Sequencing libraries were validated using the Agilent TapeStation and quantified by using Qubit Fluorometer as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). The sequencing libraries were multiplexed and clustered on a flowcell ...
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bioRxiv - Microbiology 2023Quote: ... The pooled library was cleaned using MO BIO UltraClean® PCR Clean-Up Kit (MO BIO, Carlsbad, CA) and quantified using the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Sequencing was carried out as detailed in the EMP protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The quantitative polymerase chain reaction was performed by using SYBR Green Master Mix on a LightCycler 480 PCR system (Roche Diagnostics, Indianapolis, IN, USA). Glyceraldehyde-3-phosphate dehydrogenase served as an internal control ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... We performed qRT-PCR with Roche LightCycler 480 SYBR I Green Master Mix using a Roche LightCycler 480 (Roche Applied Science, Indianapolis, IN, USA) following the manufacture’s protocol with triple technical replicates of 10 μl reactions ...
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bioRxiv - Microbiology 2023Quote: The open reading frame (orf) encoding RABV SAD L16 G was amplified from pCMV-SADL16 (27) using the Expand High FidelityPLUS PCR System (Roche Applied Science, Mannheim, Germany) with specific primers PRVGncrNDF (5’-CTA CCG CTT CAC CGA CAA CAG TCC TCA ACC ATG GTT CCT CAG GCT CTC CTG TTT GTA CC-3’ ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... Amplification of genes of interest was achieved by quantitative polymerase chain reaction (PCR) in a Roche LightCycler® 480 System (Roche Diagnostics, Burgess Hill, UK) using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Genomics 2022Quote: ... Positivity of SARS-CoV-2 infection was assessed both by PCR and measurement of specific antibodies (Cobas-Roche using Elecsys Anti-SARS-CoV-2 S). All patients gave their consent for participation in this study ...
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bioRxiv - Molecular Biology 2020Quote: A total of 4 chemosensory-related transcripts identified by RNA-seq to be expressed deferentially between forelegs and hindlegs were selected for real-time quantitative PCR (qPCR) analysis using the LightCycler® (Roche Applied Sciences, Mannheim, Germany). RNA extraction was performed as mentioned above ...
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bioRxiv - Plant Biology 2019Quote: ... the cDNA samples were diluted with 220 µl distilled water and 2 μl aliquots were amplified with the LightCycler® Nano Real-time PCR Detection System (Roche Applied Science, Tokyo, Japan) using the KOD SYBR® qRT-PCR Mix (TOYOBO) ...